Abstract: High-concentration mash fermentation has such characteristics as high cell density，high product concentration and high production rates. So it is the future aim of ethanol industry. High-concentration mash ethanol fermentation techniques have the advantages of saving process water，improving equipment utilization，and reducing energy consumption. So high-concentration mash ethanol fermentation techniques are an important way of improving ethanol production efficiency. Research on high-concentration mash ethanol fermentation techniques is very important in practical application. This paper reviews research progress of high-concentration mash ethanol fermentation techniques. The definition，advantages and influencing factors of high-concentration mash ethanol fermentation are introduced. Lowering the viscosity of fermentation mash，screening high-tolerance ethanol yeast，changing fermentation process model and adding appropriate enzymes and nutrients are important methods of achieving high-concentration mash ethanol fermentation. Among them，screening high-tolerance ethanol yeast is the key to high-concentration mash fermentation.

Key words: biological engineering, bioenergy, enzyme, ethanol, high-concentration mash fermentation

摘要： 乙醇浓醪发酵具有高细胞密度、高产物浓度和高生产速率等特点，是乙醇工业的发展目标和方向。采用乙醇浓醪发酵技术，具有节约工艺用水、提高设备利用率、降低能耗等优势，是提高乙醇发酵工业效益的重要途径。研究乙醇浓醪发酵具有十分重要的现实意义。本文综述了乙醇浓醪发酵技术研究进展，介绍了乙醇浓醪发酵定义、优势以及影响乙醇浓醪发酵的因素。指出降低发酵醪液黏度、筛选高耐性酿酒酵母、改变发酵工艺模式、添加适宜酶制剂以及营养物质是实现乙醇浓醪发酵技术的主要途径，其中筛选高耐性的酿酒酵母是实现乙醇浓醪发酵技术的关键。

关键词: 生物工程, 生物能源, 酶, 乙醇, 浓醪发酵