Chemical Industry and Engineering Progress ›› 2023, Vol. 42 ›› Issue (1): 30-39.DOI: 10.16085/j.issn.1000-6613.2022-1490

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Biotransformation of one-carbon resources by yeast

GUO Feng1(), ZHANG Shangjie1, JIANG Yujia1, JIANG Wankui1, XIN Fengxue1,2, ZHANG Wenming1,2(), JIANG Min1,2()   

  1. 1.State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China
    2.Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211816, Jiangsu, China
  • Received:2022-08-12 Revised:2022-09-16 Online:2023-02-20 Published:2023-01-25
  • Contact: ZHANG Wenming, JIANG Min

一碳资源在酵母中的利用与转化

郭峰1(), 张尚杰1, 蒋羽佳1, 姜万奎1, 信丰学1,2, 章文明1,2(), 姜岷1,2()   

  1. 1.南京工业大学生物与制药工程学院,材料化学工程国家重点实验室,江苏 南京 211816
    2.南京工业大学江苏先进生物与化学制造协同创新中心(SICAM),江苏 南京 211816
  • 通讯作者: 章文明,姜岷
  • 作者简介:郭峰(1996—),博士研究生,研究方向为甲醇酵母细胞工厂的构建。E-mail:202062112028@njtech.edu.cn
  • 基金资助:
    国家重点研发计划(2018YFA0901500);国家自然科学基金(22078151);中国科协青年人才托举工程(YESS20200174)

Abstract:

Under the current increasingly severe climate and energy crisis, there is an urgent need to reduce dependence on fossil fuels and utilize cleaner and sustainable feedstocks such as one-carbon resources including methanol, formic acid and carbon dioxide. Yeast, as a eukaryotic organism, has great potential in the biotransformation of one-carbon compounds due to the existence of organelles, which can isolate the toxic substances produced during the metabolism of one-carbon compounds. Recently, with the in-depth understanding of methanol metabolism pathways and the gradual improvement of genetic manipulation tools, many advances have been made in producing chemicals from methanol using natural methylotrophic yeasts. Also, the development of commonly used industrial yeasts such as Saccharomyces cerevisiae and Yarrowia lipolytica as synthetic methylotrophic yeasts has also achieved remarkable achievements. Herein the research progress of yeast chassis in the transformation and utilization of one-carbon raw materials is focused. Further, the current bottlenecks and potential solutions for the biotransformation of one-carbon resources are analyzed. With the development of more precise and efficient tools and the interpretation of cellular metabolic networks, the biotransformation of one-carbon resources by yeasts will play an increasingly important role in the future of green biomanufacturing.

Key words: methanol, formic acid, carbon dioxide fixation, synthetic biology, metabolic engineering

摘要:

在当前日趋严重的气候与能源危机下,传统的化工行业亟需减少对化石燃料的依赖,转而开发利用更为清洁可持续的原料,如包括甲醇、甲酸和二氧化碳等在内的一碳资源。作为真核生物的酵母由于细胞器的存在能够隔绝一碳化合物代谢过程中产生的有毒物质渗入细胞质对细胞产生毒害,在一碳化合物的生物转化中极具潜力。近年来,随着对甲醇代谢途径愈发深入地了解以及遗传操作工具的逐步完善,利用天然甲醇酵母以甲醇作为碳源生产化学品取得了重要进展。同时,开发常用的工业酵母如酿酒酵母、解脂耶氏酵母为人工甲基营养型酵母,实现将一碳资源进行高值化转化也取得了不俗的成就。本文着重讨论了酵母底盘在转化利用一碳原料方面的研究进展,在此基础上讨论并分析了目前一碳资源生物转化存在的瓶颈以及潜在的解决方案,指出随着更多精准高效的工具的开发以及对细胞代谢网络的阐释,依托酵母细胞进行一碳资源的生物转化将在未来的绿色生物制造中扮演越来越重要的角色。

关键词: 甲醇, 甲酸, 二氧化碳固定, 合成生物学, 代谢工程

CLC Number: 

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