Abstract:

Guided tissue regeneration (GTR) membrane plays pivotal roles in GTR treatment. In current study, polycaprolactone (PCL), gelatin (Gel) and nano-hydroxyapatite (n-HA) were selected as raw materials to prepare PCL/Gel/n-HA fibrous GTR membranes reinforced with different n-HA content by electrospinning technique. The effects of n-HA content on the morphology, mechanical strength, hydrophilicity and in vitro degradation rate of the three fibrous membranes were investigated. The results showed that the fibrous membranes owned good fibrous structure, and the fiber diameter was mainly distributed between 200～400nm. The diameter of the fibers increased significantly after crosslinking. With the increase of n-HA content, the aggregation of n-HA particles in the fibers and on the surface of fibers could be observed. The mechanical test showed that the tensile strength and elongation at break of the fibrous membranes increased with the n-HA content. The highest tensile strength and elongation of the PCL/Gel/n-HA membrane were 9.18MPa and 180%, respectively, when the weight percentage of n-HA was 15%. In addition, the incorporation of n-HA reduced the degradation rates of the fabricated fibrous membranes. The excellent mechanical properties and proper degradation rate of the prepared PCL/Gel/n-HA fibrous GTR membrane can meet the needs of clinical requirements.

Key words: electrospinning, polycaprolactone, gelatin, nano-hydroxyapatite

摘要：

引导组织再生膜在引导组织再生术中发挥着关键作用，高性能的引导组织再生膜能更好地促进组织再生修复。本文以纳米羟基磷灰石（n-HA）、聚己内酯（PCL）、明胶（Gel）为原料，通过静电纺丝法制备了不同含量n-HA增强的PCL/Gel/n-HA纤维膜，并对其形貌、组成、力学性能及降解性能进行了研究。SEM结果表明，纤维膜中的纤维形态良好，纤维直径大致分布于200～400nm之间，交联后纤维直径明显增加；TEM结果表明，n-HA较均匀分散在纤维中，随着n-HA含量的增加，n-HA在纤维膜表面发生聚集。力学测试结果表明，随着n-HA含量的增加显著提高了纤维膜的拉伸强度和断裂伸长率，当n-HA含量约为15%时，其拉伸强度和伸长率分别达到9.18MPa和180%。n-HA加入后，纤维膜的降解速率明显降低，n-HA含量约为15%的复合纤维膜体外降解12周以后约降解25%。本文制备的PCL/Gel/n-HA纤维膜的力学性能和降解速率能满足临床对引导组织再生膜的性能要求。

关键词: 静电纺丝, 聚己内酯, 明胶, 纳米羟基磷灰石