Chemical Industry and Engineering Progress ›› 2022, Vol. 41 ›› Issue (1): 373-381.DOI: 10.16085/j.issn.1000-6613.2021-0164

• Biochemical and pharmaceutical engineering • Previous Articles     Next Articles

Preparation and properties of FK/PLA nanofiber membrane modified with dopamine

WANG Xiqian1(), YIN Guoqiang1,2(), GUO Qingbing1, HE Ming1   

  1. 1.College of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong, China
    2.Guangzhou Key Laboratory for Efficient Utilization of Agricultural Chemicals, Guangzhou 510225, Guangdong, China
  • Received:2021-01-25 Revised:2021-05-22 Online:2022-01-24 Published:2022-01-05
  • Contact: YIN Guoqiang

多巴胺表面修饰角蛋白/聚乳酸纳米纤维膜的制备及性能

王喜倩1(), 尹国强1,2(), 郭清兵1, 何明1   

  1. 1.仲恺农业工程学院化学化工学院,广东 广州 510225
    2.广州市农用化学品高效利用重点实验室,广东 广州 510225
  • 通讯作者: 尹国强
  • 作者简介:王喜倩(1996—),女,硕士研究生,主要从事农产生物质功能化研究。E-mail: 260991192@qq.com
  • 基金资助:
    广东省教育厅高等教育“青年创新人才”项目专项资金(2018KQNCX116);广州市科技计划(201705030005);广东省普通高校特色创新类项目(2020KTSCX173)

Abstract:

The keratin/polylactic acid nanofiber membrane was surface modified by ultraviolet-induced dopamine oxidation and self-polymerization to improve the problems of poor hydrophilicity, insufficient mechanical properties and low cell activity. Through scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis, contact angle experiment, electronic universal testing machine, cell activity toxicity experiment and cell adhesion experiment, the morphology, structure, and thermal stability of the nanofiber membrane before and after surface modification Properties and thermal degradation rate, hydrophilicity, mechanical properties, cell activity toxicity and adhesion were tested and characterized. The results showed that: polydopamine successfully adhered to the surface of the fiber, the average diameter of the modified fiber increased from (356±78)nm to (507±98)nm, the contact angle decreased from 103.34? to 82.46?, the modulus of elasticity and fracture the elongation increased by 2.75—5.33MPa and 31.75%—51.50% respectively, and the cell viability increased from 72% to 221% after 24h inoculation, an increase of 149%.

Key words: dopamine surface modification, keratin, electrospinning, wound dressing

摘要:

通过紫外引发多巴胺氧化自聚生成聚多巴胺,对角蛋白/聚乳酸纳米纤维膜进行表面修饰,以改善其薄膜亲水性差、力学性能不足和细胞活性较低等问题。通过扫描电子显微镜、傅里叶变换红外光谱、热重分析、接触角实验、电子万能试验机、细胞活性毒性实验和细胞黏附性实验对表面修饰前后纳米纤维膜的形貌、结构、热稳定性和热降解率、亲水性、力学性能、细胞活性毒性和黏附性进行测试和表征。结果表明,聚多巴胺成功地黏附在纤维的表面,表面修饰后的纤维平均直径增大,由(356±78)nm增大至(507±98)nm,接触角由103.34?降至82.46?,弹性模量与断裂伸长率分别增大了2.75~5.33MPa和31.75%~51.50%,接种24h后细胞活性由72%增大至221%,增大了149%。

关键词: 多巴胺表面修饰, 角蛋白, 静电纺丝, 伤口敷料

CLC Number: 

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