Abstract:

The keratin/polylactic acid nanofiber membrane was surface modified by ultraviolet-induced dopamine oxidation and self-polymerization to improve the problems of poor hydrophilicity, insufficient mechanical properties and low cell activity. Through scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis, contact angle experiment, electronic universal testing machine, cell activity toxicity experiment and cell adhesion experiment, the morphology, structure, and thermal stability of the nanofiber membrane before and after surface modification Properties and thermal degradation rate, hydrophilicity, mechanical properties, cell activity toxicity and adhesion were tested and characterized. The results showed that: polydopamine successfully adhered to the surface of the fiber, the average diameter of the modified fiber increased from (356±78)nm to (507±98)nm, the contact angle decreased from 103.34? to 82.46?, the modulus of elasticity and fracture the elongation increased by 2.75—5.33MPa and 31.75%—51.50% respectively, and the cell viability increased from 72% to 221% after 24h inoculation, an increase of 149%.

Key words: dopamine surface modification, keratin, electrospinning, wound dressing

摘要：

通过紫外引发多巴胺氧化自聚生成聚多巴胺，对角蛋白/聚乳酸纳米纤维膜进行表面修饰，以改善其薄膜亲水性差、力学性能不足和细胞活性较低等问题。通过扫描电子显微镜、傅里叶变换红外光谱、热重分析、接触角实验、电子万能试验机、细胞活性毒性实验和细胞黏附性实验对表面修饰前后纳米纤维膜的形貌、结构、热稳定性和热降解率、亲水性、力学性能、细胞活性毒性和黏附性进行测试和表征。结果表明，聚多巴胺成功地黏附在纤维的表面，表面修饰后的纤维平均直径增大，由(356±78)nm增大至(507±98)nm，接触角由103.34?降至82.46?，弹性模量与断裂伸长率分别增大了2.75~5.33MPa和31.75%~51.50%，接种24h后细胞活性由72%增大至221%，增大了149%。

关键词: 多巴胺表面修饰, 角蛋白, 静电纺丝, 伤口敷料