Influence of rare earth element Er(Ⅲ) on performance of short-cut nitrification and its inhibition kinetics

doi:10.16085/j.issn.1000-6613.2022-1005

Abstract

Abstract:

The effect of the heavy rare earth element erbium [Er( $Ⅲ$ )] on the short-term and long-term nitrogen removal efficiency of the short-cut nitrification process was studied, and the related kinetic analysis was carried out. Short-term experiments showed that Er(Ⅲ) at the concentration of 0—10mg/L promoted the activity of AOB bacteria, 20—60mg/L Er(Ⅲ) slightly inhibited the activity of AOB bacteria, and 80—120mg/L Er(Ⅲ) showed severe inhibition on the activity of AOB bacteria. AOB bacteria would adsorb a large amount of Er(Ⅲ), and the Er(Ⅲ) removal rate was greater than 90% when the influent Er(Ⅲ) concentration was lower than 60mg/L. However,the Er(Ⅲ) removal rate gradually decreased when the influent Er(Ⅲ) concentration was higher than 60mg/L. The results of ICP-MS and EDS analysis showed that Er(Ⅲ) could be adsorbed extracellularly and uptaked intracellularly by AOB bacteria, and the extracellular adsorption was the main one. The experimental results were fitted by Vadivelu model, Hellinga model, Michaelis-Menten model and Hill model, respectively. The results showed that the inhibition kinetics process of Er (Ⅲ) on AOB bacteria can be better described by subsection fitting (0—60mg/L and 60—120mg/L) using Hill model, the R² obtained by fitting are 0.9909 and 0.9999, respectively, and the maximum matrix removal rate q_max (ΔSNPR) was 2.59mg/(g·h) and 7.15mg/(g·h), respectively. Long-term experiments showed that the performance of the short-cut nitrification system will gradually disappear with the addition of 10mg/L Er(Ⅲ), and the performance of the reaction system could not recover after the addition of Er(Ⅲ) was stopped.

Key words: rare earth impact, Er(Ⅲ), short-cut nitration, kinetics, model fitting

摘要：

研究了重稀土元素铒[Er(Ⅲ)]对短程硝化工艺的短期和长期脱氮效能影响，并进行了相关动力学分析。短期实验表明，0~10mg/L的Er(Ⅲ)对AOB菌活性呈现促进作用，20~60mg/L的Er(Ⅲ)对AOB菌活性呈现轻度抑制，80~120mg/L的Er(Ⅲ)则对AOB菌活性呈现重度抑制。AOB菌会吸附大量Er(Ⅲ)，在进水Er(Ⅲ)浓度低于60mg/L时，Er(Ⅲ)去除率均大于90%，高于60mg/L后Er(Ⅲ)去除率逐渐降低。ICP-MS和EDS分析结果表明，Er(Ⅲ)可被AOB菌胞外吸附和胞内摄取，其中又以胞外吸附为主。分别采用Vadivelu模型、Hellinga模型、Michaelis-Menten模型和Hill模型对实验结果进行拟合，结果表明通过分段拟合（0~60mg/L和60~120mg/L）Hill模型能较好的描述Er(Ⅲ)对AOB菌的抑制动力学过程，拟合得到的R²分别为0.9879和0.9999，最大基质去除速率q_max(ΔSNPR)分别为2.59mg/(g·h)和7.15mg/(g·h)。长期试验表明，10mg/L的Er(Ⅲ)将使短程硝化系统性能逐渐消失，停止添加Er(Ⅲ)后，反应系统性能也不能恢复。

关键词: 稀土影响, 铒, 短程硝化, 动力学, 模型拟合

CLC Number:

X703

LI Yun, CUI Nan, XIONG Xingxing, HUANG Zhiyuan, WANG Dongliang, XU Dan, LI Jun, LI Zebing. Influence of rare earth element Er(Ⅲ) on performance of short-cut nitrification and its inhibition kinetics[J]. Chemical Industry and Engineering Progress, 2023, 42(3): 1659-1668.

李芸, 崔楠, 熊星星, 黄志远, 王东亮, 许丹, 李军, 李泽兵. 稀土Er(Ⅲ)对短程硝化性能的影响及其抑制动力学特性[J]. 化工进展, 2023, 42(3): 1659-1668.

Figures/Tables 8

References 37

1	CHEN Z, LI Z, CHEN J, et al. Recent advances in selective separation technologies of rare earth elements: a review[J]. Journal of Environmental Chemical Engineering, 2021, 10(1): 107104.
2	郜鹏畅, 区晓琳, 陈志彪. 闽西离子吸附型稀土开采对土壤及地表水的影响[J]. 稀土, 2022, 43(5): 1-9.
	GAO Pengchang, QU Xiaolin, CHEN Zhibiao. Influence of ion adsorption rare earth mining on soil and surface water in western Fujian[J]. Chinese Rare Earths, 2022, 43(5): 1-9.
3	ZHANG D C, SU H, ANTWI P, et al. High-rate partial-nitritation and efficient nitrifying bacteria enrichment/out-selection via pH-DO controls: efficiency, kinetics, and microbial community dynamics[J]. Science of the Total Environment, 2019, 692: 741-755.
4	YANG Shuai, YANG Fenglin. Nitrogen removal via short-cut simultaneous nitrification and denitrification in an intermittently aerated moving bed membrane bioreactor[J]. Journal of Hazardous Materials, 2011, 195: 318-323.
5	ANTWI P, ZHANG D, LUO W, et al. Performance, microbial community evolution and neural network modeling of single-stage nitrogen removal by partial-nitritation/anammox process[J]. Bioresource Technology, 2019, 284: 359-372.
6	贺亮, 施万胜, 赵明星, 等.基于分点进水的垃圾渗滤液短程硝化反硝化脱氮性能[J]. 环境工程学报, 2021, 15(5): 1753-1762.
	HE Liang, SHI Wansheng, ZHAO Mingxing, et al. Nitrogen removal performance of short-cut nitrification and denitrification process based on step feeding strategy for landfill leachate treatment[J]. Chinese Journal of Environmental Engineering, 2021, 15(5): 1753-1762.
7	WU Lina, LIANG Dawei, XU Yingxing, et al. A robust and cost-effective integrated process for nitrogen and bio-refractory organics removal from landfill leachate via short-cut nitrification, anaerobic ammonium oxidation in tandem with electrochemical oxidation[J]. Bioresource Technology, 2016, 212: 296-301.
8	聂春芬, 王应军, 马星宇, 等. 固定化活性污泥实现短程硝化反硝化处理畜禽废水[J]. 环境工程学报, 2013, 7(5): 1807-1812.
	NIE Chunfen, WANG Yingjun, MA Xingyu, et al. Immobilized activated sludge to achieve shortcut nitrification-denitrification treatment of livestock wastewater[J]. Chinese Journal of Environmental Engineering, 2013, 7(5): 1807-1812.
9	SANJAYA E H, CHEN Y, GUO Y, et al. The performance of simultaneous partial nitritation, anammox, denitrification, and COD oxidation (SNADCO) method in the treatment of digested effluent of fish processing wastewater[J]. Bioresource Technology, 2022, 346: 126622.
10	ZHENG Xiong, ZHOU Weinan, WAN Rui, et al. Increasing municipal wastewater BNR by using the preferred carbon source derived from kitchen wastewater to enhance phosphorus uptake and short-cut nitrification-denitrification[J]. Chemical Engineering Journal, 2018, 344: 556-564.
11	GAO Ruitao, PENG Yongzhen, LI Jianwei, et al. Improving performance and efficiency of partial anammox by coupling partial nitrification and partial denitrification (PN/A-PD/A) to treat municipal sewage in a step-feed reactor[J]. Bioresource Technology, 2021, 341: 125804.
12	YE Liu, PENG Chengyao, TANG Bing, et al. Determination effect of influent salinity and inhibition time on partial nitrification in a sequencing batch reactor treating saline sewage[J]. Desalination, 2009, 246(1/2/3): 556-566.
13	BASSIN J P, DEZOTTI M, SANT’ANNA Jr G L. Nitrification of industrial and domestic saline wastewaters in moving bed biofilm reactor and sequencing batch reactor[J]. Journal of Hazardous Materials, 2011, 185(1): 242-248.
14	李嘉懿, 杜青平, 刘嵩, 等. 有机负荷对垃圾沥滤液短程硝化系统影响及微生物相探析[J]. 环境科学学报, 2018, 38(6): 2334-2341.
	LI Jiayi, DU Qingping, LIU Song, et al. Effect of organic loading on partial nitrification system for the treatment of fresh incineration leachate and analysis of microbial phases[J]. Acta Scientiae Circumstantiae, 2018, 38(6): 2334-2341.
15	YUAN Yao, ZHOU Zhen, JIANG Jie, et al. Partial nitrification performance and microbial community evolution in the membrane bioreactor for saline stream treatment[J]. Bioresource Technology, 2021, 320: 124419.
16	黄书昌, 朱易春, 章璋, 等. 声能密度对短程硝化启动及氨氮去除动力学的影响[J]. 化工进展, 2019, 38(9): 4335-4341.
	HUANG Shuchang, ZHU Yichun, ZHANG Zhang, et al. Effect of ultrasonic density on partial nitrification and ammonia nitrogen removal kinetics[J]. Chemical Industry and Engineering Progress, 2019, 38(9): 4335-4341.
17	ZHANG L, FAN J, NGUYEN H N, et al. Effect of cadmium on the performance of partial nitrification using sequencing batch reactor[J]. Chemosphere, 2019, 222: 913-922.
18	刘滔, 池勇志, 付海娟, 等. Zn(Ⅱ)、硫化物和苯酚对短程硝化污泥活性的抑制[J]. 环境工程学报, 2017, 11(10): 5341-5350.
	LIU Tao, CHI Yongzhi, FU Haijuan, et al. Inhibition of partial nitritation activity by Zn(Ⅱ), sulfide and phenol in activated sludge[J]. Chinese Journal of Environmental Engineering, 2017, 11(10): 5341-5350.
19	JEONG D, BAE H. Insight into functionally active bacteria in nitrification following Na⁺ and Mg²⁺ exposure based on 16S rDNA and 16S rRNA sequencing[J]. Science of the Total Environment, 2021, 758: 143592.
20	王志高, 王金荣, 彭文博, 等. 膜分离技术处理离子型稀土矿稀土开采废水[J]. 稀土, 2017, 38(1): 102-107.
	WANG Zhigao, WANG Jinrong, PENG Wenbo, et al. Membrane separation technology to treat ionic rare earth ore rare earth mining wastewater[J]. Chinese Rare Earths, 2017, 38(1): 102-107.
21	SU Hao, ZHANG Dachao, ANTWI P, et al. Exploring potential impact (s) of cerium in mining wastewater on the performance of partial-nitrification process and nitrogen conversion microflora[J]. Ecotoxicology and Environmental Safety, 2021, 209: 111796.
22	赖城, 周豪, 张大超, 等. 重稀土元素钇对短程反硝化工艺的影响[J]. 中国环境科学, 2021, 41(7): 3221-3228.
	LAI Cheng, ZHOU Hao, ZHANG Dachao, et al. Effect of heavy rare earth element yttrium on partial denitrification process： performance, kinetics and bacterial activity[J]. China Environmental Science, 2021, 41(7): 3221-3228.
23	VADIVELU V M, YUAN Z G, FUX C, et al. The inhibitory effects of free nitrous acid on the energy generation and growth processes of an enriched nitrobacter culture[J]. Environmental Science &Technology, 2006, 40(14): 4442-4448.
24	HELLINGA C, VAN LOOSDRECHT M C M, HEIJNEN J J. Model based design of a novel process for nitrogen removal from concentrated flows[J]. Mathematical and Computer Modelling of Dynamical Systems, 1999, 5(4): 351-371.
25	孙琪, 赵白航, 范飒, 等. 重金属Ni(Ⅱ)对厌氧氨氧化脱氮性能的影响及其动力学特征变化[J]. 环境科学, 2020, 41(06): 2779-2786.
	SUN Qi, ZHAO Baihang, FAN Sa, et al. Effect of Ni(Ⅱ) on anaerobic ammonium oxidation and changes in kinetics[J].Environmental Science, 2020, 41(6): 2779-2786.
26	YANG Lijuan, Guanjun NAN, MENG Xianxin, et al. Study on the interaction between lovastatin and three digestive enzymes and the effect of naringin and vitamin C on it by spectroscopy and docking methods[J]. International Journal of Biological Macromolecules, 2020, 155: 1440-1449.
27	CHANG S I, REINFELDER J R. Bioaccumulation, subcellular distribution, and trophic transfer of copper in a coastal marine diatom[J]. Environmental Science &Technology, 2000, 34(23): 4931-4935.
28	SU Hao, ZHANG Dachao, ANTWI P, et al. Effects of heavy rare earth element (yttrium) on partial-nitritation process, bacterial activity and structure of responsible microbial communities[J]. Science of the Total Environment, 2020, 705: 135797.
29	KHAN A A, GANI A, MASOODI F A, et al. Structural, rheological, antioxidant, and functional properties of β–glucan extracted from edible mushrooms Agaricus bisporus, Pleurotus ostreatus and Coprinus attrimentarius [J]. Bioactive Carbohydrates and Dietary Fibre,2017,11: 67-74.
30	FUSTE N P, GUASCH M, GUILLEN P, et al. Barley β-glucan accelerates wound healing by favoring migration versus proliferation of human dermal fibroblasts[J]. Carbohydrate Polymers, 2019, 210: 389-398.
31	CORSINO S F, CAPODICI M, TORREGROSSA M, et al. Fate of aerobic granular sludge in the long-term: the role of EPSs on the clogging of granular sludge porosity[J]. Journal of Environmental Management, 2016, 183: 541-550.
32	POZNVAK T, BAUTISTA G L, CHAIREZ I, et al. Decomposition of toxic pollutants in landfill leachate by ozone after coagulation treatment[J]. Journal of Hazardous Materials, 2008, 152(3): 1108-1114.
33	LEE D J, CHEN Y Y, SHOW K Y, et al. Advances in aerobic granule formation and granule stability in the course of storage and reactor operation[J]. Biotechnology Advances, 2010, 28(6): 919-934.
34	SU H, ZHANG D C, ANTWI P, et al. Adaptation, restoration and collapse of anammox process to La(Ⅲ) stress: performance, microbial community, metabolic function and network analysis[J]. Bioresource Technology, 2021, 325: 124731.
35	KOTRBA P, INUI M, YUKAWA H. Bacterial phosphotransferase system (PTS) in carbohydrate uptake and control of carbon metabolism[J]. Journal of Bioscience and Bioengineering, 2001, 92(6): 502-517.
36	SU H, ZHANG D C, ANTWI P, et al. Unraveling the effects of light rare-earth element (Lanthanum (Ⅲ)) on the efficacy of partial-nitritation process and its responsible functional genera[J]. Chemical Engineering Journal, 2021, 408: 127311.
37	YUE Yan, QI Lin, LI Yan, et al. Negative effects of rare earth oxide nanoparticles of La₂O₃, Nd₂O₃, and Gd₂O₃ on the ammonia-oxidizing microorganisms[J]. Journal of Soils and Sediments, 2020, 20(8): 3114-3123.

Er(Ⅲ)浓度 /mg·L^-1	元素质量分数/%
Er(Ⅲ)浓度 /mg·L^-1	C	O	Er	其他
0	33.88	33.66	0	32.46
10	36.85	38.02	1.56	23.57
60	30.58	26.16	22.46	20.80
80	21.25	26.69	29.03	23.03

Er(Ⅲ)浓度 /mg·L^-1	元素质量分数/%
Er(Ⅲ)浓度 /mg·L^-1	C	O	Er	其他
0	33.88	33.66	0	32.46
10	36.85	38.02	1.56	23.57
60	30.58	26.16	22.46	20.80
80	21.25	26.69	29.03	23.03

浓度范围/mg·L^-1	Vadivelu模型				Hellinga模型
浓度范围/mg·L^-1	R²	q_max	K	a	R²	q_max	K_I
0~120	0.9613	6.23	7.98×10^-28	14.89	0.6805	7.96	35.54
0~60	0.7054	7.75	0.005	1.22	0.9195	7.21	124.11
60~120	0.9938	4.35×10⁷	9.20×10^-10	8.98	0.3299	1.03×10⁵	0.001
浓度范围/mg·L^-1	Hill模型				Michaelis-Menten模型
浓度范围/mg·L^-1	R²	q_max	K	n	R²	q_max	K
0~120	0.8912	8.75	65.23	3.25	0.8740	1.01×10¹³	1.54×10¹⁴
0~60	0.9879	2.59	15.66	1.43	0.9920	2.95	17.70
60~120	0.9999	7.15	62.27	19.21	0.6106	96.94	1360.32

浓度范围/mg·L^-1	Vadivelu模型				Hellinga模型
浓度范围/mg·L^-1	R²	q_max	K	a	R²	q_max	K_I
0~120	0.9613	6.23	7.98×10^-28	14.89	0.6805	7.96	35.54
0~60	0.7054	7.75	0.005	1.22	0.9195	7.21	124.11
60~120	0.9938	4.35×10⁷	9.20×10^-10	8.98	0.3299	1.03×10⁵	0.001
浓度范围/mg·L^-1	Hill模型				Michaelis-Menten模型
浓度范围/mg·L^-1	R²	q_max	K	n	R²	q_max	K
0~120	0.8912	8.75	65.23	3.25	0.8740	1.01×10¹³	1.54×10¹⁴
0~60	0.9879	2.59	15.66	1.43	0.9920	2.95	17.70
60~120	0.9999	7.15	62.27	19.21	0.6106	96.94	1360.32

浓度范围 /mg·L^-1	Hill模型（胞外）				Hill模型（胞内）
浓度范围 /mg·L^-1	R²	q_max	K	n	R²	q_max	K	n
0~120	0.9105	7.45	5.95	20.51	0.9136	9.34	0.49	5.37
0~60	0.9755	2.99	1.93	0.86	0.9977	3.66	0.34	1.85
60~120	0.9999	7.15	5.94	57.08	0.9999	7.15	0.47	32.50