全局转录调控在细胞工厂构建中的应用与进展

doi:10.16085/j.issn.1000-6613.2020-1907

化工进展 ›› 2021, Vol. 40 ›› Issue (3): 1248-1251.DOI: 10.16085/j.issn.1000-6613.2020-1907

全局转录调控在细胞工厂构建中的应用与进展

周子康¹^,²(), 许平¹^,²()

^1.上海交通大学生命科学技术学院，上海 200240
^2.上海交通大学微生物代谢国家重点实验室，上海 200240

收稿日期:2020-09-19 出版日期:2021-03-05 发布日期:2021-03-17
通讯作者: 许平
作者简介:周子康（1988—），男，博士，研究方向为工业微生物学。E-mail：tony_zhou@sjtu.edu.cn。

Application and progress of global transcription regulation in microbial cell factory construction

ZHOU Zikang¹^,²(), XU Ping¹^,²()

^1.School of Life Science and Technology, Shanghai Jiaotong University, Shanghai 200240, China
^2.State Key Laboratory of Microbial Metabolism, Shanghai Jiaotong University, Shanghai 200240, China

Received:2020-09-19 Online:2021-03-05 Published:2021-03-17
Contact: XU Ping

摘要/Abstract

摘要：

全局转录调控是一种通过重新编程基因转录来获得新的细胞表型的重要技术方法。本文介绍了通过DNA重组、易错PCR等技术对细胞中的转录元件进行多轮突变修饰，改变RNA聚合酶的转录效率以及对启动子的亲和能力，在整体水平上使细胞的转录水平发生改变，从而获得更加契合实际需求的细胞表型。指出全局转录调控技术不仅可以快速优化代谢途径、提高目标化合物的产量，亦可提高菌株耐受性，在代谢工程领域具有独特的优势，并已被成功应用于不同化合物的细胞工厂构建中。

关键词: 全局转录调控工程, 代谢工程, 定向进化, 耐受性

Abstract:

Global transcription machinery engineering is an important technical method to reprogram gene transcription to obtain new cell phenotypes. Through DNA recombination, error-prone PCR and other techniques, multiple rounds of mutation modification of transcription elements in cells are carried out to change the conversion efficiency of RNA polymerase and the affinity for promoters is discussed. They change the transcription of cells on the whole genomic level, thereby evolving a cell phenotype that meets actual needs. It is indicated that global transcription machinery engineering can not only rapidly optimize metabolic pathways and increase the yield of target compounds, but also improve strain tolerances. These advantages have been successfully applied to the construction of microbial cell factories for various compounds production.

Key words: global transcription machinery engineering, metabolic engineering, directed evolution, toleration

中图分类号:

Q939

周子康, 许平. 全局转录调控在细胞工厂构建中的应用与进展[J]. 化工进展, 2021, 40(3): 1248-1251.

ZHOU Zikang, XU Ping. Application and progress of global transcription regulation in microbial cell factory construction[J]. Chemical Industry and Engineering Progress, 2021, 40(3): 1248-1251.

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全局转录调控在细胞工厂构建中的应用与进展

Application and progress of global transcription regulation in microbial cell factory construction

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