秸秆与猪粪混合高固厌氧消化产气性能及关键微生物分析

doi:10.16085/j.issn.1000-6613.2019-1311

摘要/Abstract

摘要：

高固厌氧消化（HS-AD）是处理木质纤维素类原料和其他高固含率有机原料的有效方式之一。本文以节能高效为出发点，研究秸秆和猪粪为混合原料，两者不同配比（秸秆/猪粪的质量比2∶1、1∶1和1∶2）条件下，反应器的运行情况及关键微生物变化情况。结果表明：原料的配比对高固厌氧消化的产气量有明显影响。相比于其他两组实验，秸秆和猪粪的配比为2∶1时，累计产气量最大为229.66L，最终甲烷含量稳定在60.7%左右，转化为单位VS产甲烷量为131.8mL/g VS。同时，反应过程中液相性质（pH、VFA、TIC）的变化，也说明秸秆和猪粪配比为2∶1时，运行平稳且产气较好。另外，微生物分析结果显示，秸秆和猪粪配比为2∶1的实验组，在实验启动的前期，甲烷八叠球菌（Methanosarcinaceae）的相对丰度较高，并且细菌和古菌群落的丰富度和多样性都优于其他两组。

关键词: 高固厌氧消化, 秸秆, 猪粪, 微生物分析

Abstract:

A single raw material, such as crop straw or livestock manure, is subjected to anaerobic digestion, and the gas production is not satisfactory. Based on energy conservation and efficiency, two raw materials were mixed to study the effect of different mass ratios of straw and pig manure（2∶1, 1∶1 and 1∶2）on anaerobic digestion. The results showed that the ratio of raw materials had a significant effect on the gas production of anaerobic digestion. Among the three ratios, when the ratio of straw to pig manure was 2∶1, the cumulative biogas production was the largest, reaching 229.66L, and the final methane content was stable at about 60.71%, which meant the amount of methane produced per unit VS was 131.8mL/g VS. At the same time, the change of biogas liquid properties (pH, VFA, TIC) from the reaction process also indicated that when the ratio of straw to pig manure was 2∶1, the experiment ran smoothly and the gas production was better. In addition, microbiological analysis showed that in the experimental group with the ratio of 2∶1, the relative abundance of Methanosarcinaceae was higher during the first three weeks of the experiment, and the richness and diversity of bacteria and archaea communities were higher than other two groups.

Key words: high solid anaerobic digestion, straw, pig manure, microbial community analysis

中图分类号:

傅国志, 郭文阳, 马宗虎, 刘蔚, 李博凯, 孙子滟, 王祯欣, 李叶青. 秸秆与猪粪混合高固厌氧消化产气性能及关键微生物分析[J]. 化工进展, 2020, 39(8): 3386-3394.

Guozhi FU, Wenyang GUO, Zonghu MA, Wei LIU, Bokai LI, Ziyan SUN, Zhenxin WANG, Yeqing LI. Analysis of gas generation performance and key microorganisms of high solid anaerobic digestion of straw mixed with pig manure[J]. Chemical Industry and Engineering Progress, 2020, 39(8): 3386-3394.

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