激活剂促进微生物降解偶氮、蒽醌和三苯甲烷类染料研究进展

doi:10.16085/j.issn.1000-6613.2018-2089

摘要/Abstract

摘要：

目前利用生物法处理印染废水应用非常广泛。激活剂作为能提高生物酶活性、促进酶促反应、提高生物降解效率的有效物质，在提高含染料废水生物处理效率的研究中具有重要意义。本文综合介绍了多种不同种类的激活剂，以及它们分别对菌株、菌群脱色偶氮、蒽醌、三苯甲烷等三大类不同结构染料的激活促进作用。综合分析近年来国内外研究成果，了解到目前生物处理染料常用的激活剂主要为金属离子、氧化还原介体等。综合来看，虽然目前激活剂促进微生物降解染料研究方面已有一些成果，但还存在使用单一菌种和单一激活剂无法有效处理复合染料污染废水，无法有效缩短处理时间等问题。提出针对不同结构染料及复合污染染料开发高效混合菌群及其最适合的激活剂种类、组合等是今后的研究方向。研究成果将为激活剂在实际含染料废水生物处理过程中更好地应用提供理论基础。

关键词: 激活剂, 染料, 生物降解

Abstract:

Currently, the biological method for the treatment of printing and dyeing wastewater is widely used. Activator is of great significance in improving the biological treatment efficiency of dye-containing wastewater as an effective substance which can increase the activity of biological enzymes, promote the enzymatic reaction rate and enhance the biodegradation efficiency. In this paper, several different kinds of activators were introduced, and their activation and promotion effects on decolorization of azo, anthraquinone and triphenylmethane by strains and flora were discussed.The domestic and foreign researches showed that the current activators for biological treatment of dyes mainly include metal ions, redox mediators and so on. There are some achievements in the research of activators to promote microbial degradation of dyes. However, there are still some problems that complex dyeing wastewater cannot be effectively treated by using a single strain or a single activator, and the treatment time can not be effectively shortened. Therefore, the future research direction would be the development of high-efficiency mixed bacterial flora and its most suitable activator types and combinations for different structural dyes and composite dyes. The research would provide a theoretical basis for the better application of activators in the actual biological treatment of dye-containing wastewater.

Key words: activator, dye, biodegradation

中图分类号:

X172

王怡琴, 谢学辉, 郑秀林, 张庆云, 许可欣, 柳建设. 激活剂促进微生物降解偶氮、蒽醌和三苯甲烷类染料研究进展[J]. 化工进展, 2019, 38(06): 2968-2976.

Yiqin WANG, Xuehui XIE, Xiulin ZHENG, Qingyun ZHANG, Kexin XU, Jianshe LIU. Advances in research on activators promoting microbial degradation of dyes[J]. Chemical Industry and Engineering Progress, 2019, 38(06): 2968-2976.

图/表 1

参考文献 43

1	温沁雪, 王进, 郑明明, 等 . 印染废水深度处理技术的研究进展及发展趋势[J]. 化工环保, 2015, 35(4): 363-369．
	WHEN Q X , WANG J , ZHENG M M , et al ．Research progresses and development trends of technologies for dyeing wastewater advanced treatment[J]．Environmental Protection of Chemical Industry, 2015, 35(4): 363-369．
2	刘有勋．染料的环境污染及其处理方法[J]. 中南论坛：综合版, 2006（2）： 98-107．
	LIU Y X ．Dye environmental pollution and its treatment method[J]. Central South Forum：Comprehensive Edition, 2006（2）： 98-107．
3	陈文华, 李刚, 许方程, 等．染料废水污染现状及处理方法研究进展[J]．浙江农业科学, 2014, 1(2): 1-5．
	CHEN W H , LI G , XU F C , et al ．Research progress on pollution status and treatment methods of dye wastewater[J]．Zhejiang Agricultural Science, 2014, 1(2): 1-5．
4	陈婵维, 付忠田, 于洪蕾, 等 . 染料废水处理技术进展[J]．环境保护与循环经济, 2010, 30(4): 37-40．
	CHEN C W , FU Z T , YU H L , et al ．Progress in dye wastewater treatment technology[J]．Environmental Protection and Circular Economy, 2010, 30(4): 37-40．
5	SATILMIS B , UYAR T ．Amine modified electrospun PIM-1 ultrafine fibers for an efficient removal of methyl orange from an aqueous system[J]．Applied Surface Science, 2018, 453：220-229．
6	丁凤 . 零价铁催化过硫酸盐高级氧化工艺高效降解印染废水[D]. 吉林: 吉林大学, 2017．
	DING F ．Degradation of printing and dyeing wastewater by zero-valent iron activated persulfate treatment process[D]．Jilin：Jilin University, 2017．
7	SAMANTA M , MUKHERJEE M , GHORAI U K , et al ．Ultrasound assisted catalytic degradation of textile dye under the presence of reduced graphene oxide enveloped copper phthalocyanine nanotube[J]．Applied Surface Science,2018,449:113-121.
8	丁凤友 . 几种分散染料的生物降解特性研究[D]. 上海: 东华大学, 2016.
	DING F Y ．Study on the biodegradation characteristics of several disperse dyes[D]. Shanghai: Donghua University, 2016.
9	陈跃．染料废水处理技术及研究趋势[J]．黄石理工学院学报, 2011, 27(1): 8-14．
	CHEN Y ．Treatment technology of dyestuff wastewater and its research trend[J]．Journal of Huangshi Institute of Technology, 2011, 27(1): 8-14．
0	DU L N , LI G , ZHAO Y H , et al ．Efficient metabolism of the azo dye methyl orange by Aeromonas sp. strain DH-6：characteristics and partial mechanism[J]．International Biodeterioration & Biodegradation, 2015, 105: 66-72．
11	LI T , GUTHRIE J T ．Colour removal from aqueous solutions of metal-complex azo dyes using bacterial cells of Shewanella strain J18 143[J]．Bioresource Technology, 2010, 101(12): 4291-4295．
12	殷亚妮, 吉祖筠．激活剂和抑制剂对酶活性的影响[J]．中学生物教学, 2013(9): 50-52．
	YIN Y N , JI Z Y ．Effects of activators and inhibitors on enzyme activity[J]．Middle School Biology Teaching, 2013(9): 50-52．
13	TELKE A A , KALYANI D C , DAWAKR V V , et al ．Influence of organic and inorganic compounds on oxidoreductive decolorization of sulfonated azo dye C.I. Reactive Orange 16[J]．Journal of Hazardous Materials, 2009, 172(1): 298-309．
14	R Ö ÜREK , PAZARLIOGLU N K ．Production and stimulation of manganese peroxidase by immobilized Phanerochaete chrysosporium [J]．Process Biochemistry, 2005, 40(1): 83-87．
15	WAGHMODE T R , KURADE M B , KHANDARE R V , et al ．A sequential aerobic/microaerophilic decolorization of sulfonated mono azo dye Golden Yellow HER by microbial consortium GG-BL[J]．International Biodeterioration & Biodegradation, 2011, 65(7): 1024-1034．
16	AYED L , MAHDHI A , CHEREF A , et al ．Decolorization and degradation of azo dye Methyl Red by an isolated Sphingomonas paucimobilis: biotoxicity and metabolites characterization[J]．Desalination, 2011, 274(1): 272-277．
17	PHUGARE S , KALYANI D C , PATIL A V , et al ．Textile dye degradation by bacterial consortium and subsequent toxicological analysis of dye and dye metabolites using cytotoxicity, genotoxicity and oxidative stress studies[J]．Journal of Hazardous Materials, 2011, 186(1): 713-723．
18	JOSHI S M , INAMDAR S A , TELKE A A , et al ．Exploring the potential of natural bacterial consortium to degrade mixture of dyes and textile effluent[J]．International Biodeterioration & Biodegradation, 2010, 64(7): 622-628．
19	ASGHER M , BHATTI H N , SHAH S A H , et al ．Decolorization potential of mixed microbial consortia for reactive and disperse textile dyestuffs[J]．Biodegradation, 2007, 18(3): 311-316．
20	张晖, 曹志强, 吴峰．零价铁对活性艳红X-3B的脱色研究[J]. 水处理技术, 2004, 30(4): 221-223．
	ZHANG H , CAO Z Q , WU F ．Decoloration of the dye reactive red X-3B by zero-valent iron[J]．Technology of Water Treatment, 2004, 30(4): 221-223．
21	汤文琪．Fe⁰-厌氧微生物联合体系处理活性染料的试验研究[D]. 湘潭: 湘潭大学, 2009．
	TANG W Q ．An experimental study of reactive dyes degradation using an integrated microbial- Fe⁰ system[D]．Xiangtan: Xiangtan University, 2009．
22	范凤霞．混合菌群FF对活性黑5的脱色降解研究[D]. 上海: 东华大学, 2013．
	FAN F X ．Study on decolorization and degradation of reactive Black 5 by the flora FF[D]. Shanghai: Donghua University, 2013．
23	LU L , ZENG G , FAN C , et al ．Characterization of a laccase-like multicopper oxidase from newly isolated Streptomyces sp. C1 in agricultural waste compost and enzymatic decolorization of azo dyes[J]. Biochemical Engineering Journal, 2013, 72(11): 70-76．
24	DOS S A B, BISSCHOPS I A , CERVANTES F J , et al ．Effect of different redox mediators during thermophilic azo dye reduction by anaerobic granular sludge and comparative study between mesophilic (30℃) and thermophilic (55℃) treatments for decolorization of textile wastewaters[J]. Chemosphere, 2004, 55(9): 1149-1157．
25	MUNARI F , CALLONI R , DILLON A J P ．Decolorization of textile dyes by enzymatic extract and submerged cultures of Pleurotus sajorcaju [J]．World Journal of Microbiology & Biotechnology, 2008, 24(8): 1383-1392．
26	AGHAIE K M , FOROOTANFAR H , MOSHFEGH M , et al ．Decolorization of some synthetic dyes using optimized culture broth of laccase producing ascomycete Paraconiothyrium variabile[J]．Biochemical Engineering Journal, 2012, 60(60): 9-15．
27	侯红漫．白腐真菌Pleurotus ostreatus漆酶及对蒽醌染料和碱木素脱色的研究[D]. 辽宁: 大连理工大学, 2003．
	HOU H M ．Study on the laccase from white-rot fungus Pleurotus ostreaues and its decolorization for anthraquinone dye and alkali lignin[D]．Liaoning: Dalian University of Technology, 2003．
28	曹立芳．白腐真菌的培养及对蒽醌染料脱色的研究[D]. 重庆: 重庆工商大学, 2009．
	CAO L F ．Study on culture of white rot fungi and study on decolorization of anthraquinone dyes[D]. Chongqing: Chongqing Technology and Business University, 2009．
29	COUTO S R , RIVELA I , MURIOZ M R , et al ．Stimulation of ligninolytic enzyme production and the ability to decolourise Poly R-478 in semi-solid-state cultures of Phanerochaete chrysosporium [J]．Bioresource Technology, 2000, 74(2): 159-164．
30	甘莉, 路则洋, 林加奖．EDTA增强Cr(Ⅵ)共存时Burkholderia Cepacia降解孔雀绿的效率[J]．环境科学学报, 2017, 37(12): 4610-4616．
	GAN L , LU Z Y , LIN J J ．EDTA improves the biodegradation efficiency of malachite green using Burkholderia cepacia in the presence of Cr(Ⅵ )[J]．Acta Scientiae Circumstantiae, 2017, 37(12): 4610-4616．
31	蓝靖, 王芳, 黄锡荣, 等．过氧化氢调控藜芦醇介导木质过氧化物催化氧化邻苯三酚红研究[J]．化学学报, 2006, 64(6): 463-468．
	LAN J , WANG F , HUANG X R , et al ．Studies on the hydrogen peroxide regulated veratryl alcohol mediated oxidation of pyrogallol red catalyzed by lignin peroxidase[J]．Acta Chimica Sinica, 2006, 64(6): 463-468．
32	张淼．蒽醌介体强化偶氮染料的脱色特性及机理研究[D]. 哈尔滨: 东北林业大学, 2016．
	ZHANG M ．Study on the decolorizing characteristics and the catalysis mechanism of redox mediator during the azo dyes biodegradation processes[D]．Harbin: Northeast Forestry University, 2016．
33	崔姗姗, 徐宏勇, 周思辰, 等．氧化还原介体对偶氮染料厌氧脱色的影响[J]. 哈尔滨理工大学学报, 2008, 13(2): 104-106．
	CUI S S , XU H Y , ZHOU S C , et al ．Effects of different redox mediators on the anaerobic reduction of azo dyes[J]．Journal Harbin Univisity Science and Technology, 2008, 13(2): 104-106．
34	GUO J , ZHOU J , WANG D , et al ．The new incorporation bio-treatment technology of bromoamine acid and azo dyes wastewaters under high-salt conditions[J]．Biodegradation, 2008, 19(1): 93-98．
35	JENSEN H L ．Carbon nutrition of some microorganisms decomposing halogen-substituted aliphatic acids[J]．Acta Agriculturae Scandinavica, 1963, 13(4): 404-412．
36	KURADE M B , WAGHMODE T R , GOVINDWAR S P ．Preferential biodegradation of structurally dissimilar dyes from a mixture by Brevibacillus laterosporus [J]．Journal of Hazardous Materials, 2011, 192(3): 1746-1755．
37	TELKE A , KALYANI D , JADHAV J , et al ．Kinetics and mechanism of Reactive Red 141 degradation by a bacterial isolate Rhizobium radiobacter MTCC 8161[J]．Acta Chimica Slovenica, 2008, 55(2): 320-329．
38	HOLKAR C R , PANIDIT A B , PINJARI D V ．Kinetics of biological decolorisation of anthraquinone based Reactive Blue 19 using an isolated strain of Enterobacter sp. F NCIM 5545[J]．Bioresource Technology, 2014, 173(173): 342-351．
39	RYBCZYRISKA T K , ŚWIECILO A , SZYCHOWSK K A , et al ．Comparative study of eco- and cytotoxicity during biotransformation of anthraquinone dye Alizarin Blue Black B in optimized cultures of microscopic fungi[J]．Ecotoxicology & Environmental Safety, 2017, 147: 776-787．
40	KHAN Z , JAIN K , SONI A , et al ．Microaerophilic degradation of sulphonated azo dye–Reactive Red 195 by bacterial consortium AR1 through co-metabolism[J]．International Biodeterioration & Biodegradation, 2014, 94: 167-175．
41	刘娜, 谢学辉, 杨波, 等．生物强化水解酸化过程前后微生物群落结构变化[J]．环境工程学报, 2016, 10(6): 2769-2774．
	LIU N , XIE X H , YANG B , et al ．Changes of microbial community in hydrolysis acidification processes before and after bioaugmentation[J]．Chinese Journal of Environmental Engineering, 2016, 10(6): 2769-2774．
42	XIE X H , LIU N , PING J , et al ．Illumina MiSeq sequencing reveals microbial community in HA process for dyeing wastewater treatment fed with different co-substrates[J]．Chemosphere, 2018, 201: 578-585．
43	张庆云, 谢学辉, 俞承志, 等．不同共代谢基质对活性黑5脱色菌群脱色性能及群落结构的影响[J]．生态学杂志, 2017, 36(9): 2572-2580．
	ZHANG Q Y , XIE X H , YU C Z , et al ．Effects of different co-metabolic substrates on the decolorization of reactive Black 5 by bacteria and the community structure of bacterial flora[J]．Chinese Journal of Ecology, 2017, 36(9): 2572-2580．

微生物名称	激活剂	降解效果	参考文献
Bacillus sp. ADR	Ca²⁺	脱色率相比于对照组增长了1.5倍	[13]
	3,4-二甲氧苄醇、邻甲苯胺	分别使C.I活性橙16脱色率提高了1.51、1.30倍	[13]
黄孢原毛平革菌	Mn²⁺	极短时间能脱色偶氮染料	[14]
	Tween 80	3天后染料脱色率为99%（对照组75%）	[29]
Streptomyces sp. C1	丁香醛	短时间内促进菌株快速脱色降解偶氮染料	[23]
P. sajor-caju PS2001	蒽醌类染料	作为漆酶的底物来促进非底物染料的脱色	[25]
Paraconiothyrium variabiele	HBT	染料的脱色率随HBT浓度的增加而提高	[26]
白腐真菌	ABTS	非酶底物类染料SN 4R脱色速率明显提高、时间缩短	[27]
	ABTS	提高漆酶活性从而提高分散活性紫HFRL的脱色率	[28]
	VA	明显提高SN 4R的脱色率、缩短脱色时间	[27]
	VA	VA能作为氧化还原介体物质激活白腐真菌产生Lip从而促进脱色降解PR	[31]
	愈创木酚	提高漆酶活性从而提高分散活性紫HFRL的脱色率	[28]
Rhizobium radiobacter mtcc 8161	酵母提取物、尿素	活性红141脱色率由2%升高至86%	[37]
Enterobacter sp.F	葡萄糖	加入葡萄糖后活性艳蓝19脱色率显著增强（不加葡萄糖时无法脱色）	[38]
Haematonectria haematococca BwⅢ43和K37	干酪素水解物	一周的脱色率均达80%~90%	[39]

微生物名称	激活剂	降解效果	参考文献
Bacillus sp. ADR	Ca²⁺	脱色率相比于对照组增长了1.5倍	[13]
	3,4-二甲氧苄醇、邻甲苯胺	分别使C.I活性橙16脱色率提高了1.51、1.30倍	[13]
黄孢原毛平革菌	Mn²⁺	极短时间能脱色偶氮染料	[14]
	Tween 80	3天后染料脱色率为99%（对照组75%）	[29]
Streptomyces sp. C1	丁香醛	短时间内促进菌株快速脱色降解偶氮染料	[23]
P. sajor-caju PS2001	蒽醌类染料	作为漆酶的底物来促进非底物染料的脱色	[25]
Paraconiothyrium variabiele	HBT	染料的脱色率随HBT浓度的增加而提高	[26]
白腐真菌	ABTS	非酶底物类染料SN 4R脱色速率明显提高、时间缩短	[27]
	ABTS	提高漆酶活性从而提高分散活性紫HFRL的脱色率	[28]
	VA	明显提高SN 4R的脱色率、缩短脱色时间	[27]
	VA	VA能作为氧化还原介体物质激活白腐真菌产生Lip从而促进脱色降解PR	[31]
	愈创木酚	提高漆酶活性从而提高分散活性紫HFRL的脱色率	[28]
Rhizobium radiobacter mtcc 8161	酵母提取物、尿素	活性红141脱色率由2%升高至86%	[37]
Enterobacter sp.F	葡萄糖	加入葡萄糖后活性艳蓝19脱色率显著增强（不加葡萄糖时无法脱色）	[38]
Haematonectria haematococca BwⅢ43和K37	干酪素水解物	一周的脱色率均达80%~90%	[39]

[1]	张志伟, 杨伟鑫, 张隽佶. 长波长驱动光开关染料分子研究进展[J]. 化工进展, 2023, 42(8): 4058-4075.
[2]	吴锋振, 刘志炜, 谢文杰, 游雅婷, 赖柔琼, 陈燕丹, 林冠烽, 卢贝丽. 生物质基铁/氮共掺杂多孔炭的制备及其活化过一硫酸盐催化降解罗丹明B[J]. 化工进展, 2023, 42(6): 3292-3301.
[3]	田芳, 郭光, 丁克强, 杨凤, 刘翀, 王慧雅. 降解偶氮染料嗜盐菌的分离、降解特性及机制[J]. 化工进展, 2023, 42(4): 2115-2121.
[4]	李光文, 华渠成, 黄作鑫, 达志坚. 聚甲基丙烯酸酯类黏度指数改进剂的研究进展[J]. 化工进展, 2023, 42(3): 1562-1571.
[5]	许春树, 姚庆达, 梁永贤, 周华龙. 金属-有机框架材料结构设计及其对合成染料的吸附性能[J]. 化工进展, 2023, 42(10): 5322-5338.
[6]	王妍, 秦振平, 刘越, 张文海, 郭红霞. 环糊精原位改性MoS₂管式陶瓷复合膜的制备及性能[J]. 化工进展, 2023, 42(10): 5373-5380.
[7]	杨凯璐, 陈明星, 王新亚, 张威, 肖长发. 染料废水处理用纳滤膜制备及改性研究进展[J]. 化工进展, 2023, 42(10): 5470-5486.
[8]	付佳, 谌伦建, 徐冰, 华绍烽, 李从强, 杨明坤, 邢宝林, 仪桂云. 模拟煤炭气化废水中苯酚的微生物降解[J]. 化工进展, 2023, 42(1): 526-537.
[9]	刘雅娟. 浸没式PAC-AMBRs系统中PAC缓解膜污染的研究进展[J]. 化工进展, 2023, 42(1): 457-468.
[10]	范娇, 谢学辉, 秦艳, 陈小光, 方英荣, 杨珊珊, 赵江贵, 莫浩楠, 郑航蜜, 刘娜, 张庆云, 宋新山. 功能菌群DDMZ1共代谢脱色降解不同结构染料差异效应[J]. 化工进展, 2022, 41(8): 4449-4463.
[11]	岳瑶, 蒲梦凡, 王文瑞, 赵俭波, 曹辉. 聚天冬氨酸凝胶的制备及生物降解性[J]. 化工进展, 2022, 41(8): 4491-4497.
[12]	江汝清, 余广炜, 王玉, 黎长江, 邢贞娇. 酸改性猪粪生物炭的制备及其对直接红23染料的吸附性能[J]. 化工进展, 2022, 41(12): 6489-6499.
[13]	陈毓, 王佳佳, 汤琳. 漂浮型氮化碳光催化剂CNx@mEP的制备及性能[J]. 化工进展, 2022, 41(12): 6477-6488.
[14]	汪润民, 张晓东, 徐成华, 于丹丹, 余冉. 高效重质石油降解菌群构建及降解性能评价[J]. 化工进展, 2022, 41(10): 5653-5660.
[15]	李泽辉, 崔恒, 王军. 氯化聚氯乙烯复合纳滤膜的制备及其在模拟RB5染料废水处理中的应用[J]. 化工进展, 2021, 40(S1): 456-465.