关键词: 安丝菌素P-3, 珍贵橙色束丝放线菌, 发酵, 合成, 优化

Abstract: Solid-state fermentation of Actinosynnema pretiosum was investigated to establish an optimal process for synthesis of AP-3. The agricultural and sideline products (cornstarch, cornmeal, cottonseed meal, soybean meal, bean cake powder, rapeseed meal, wheat bran and rice bran) were used as raw materials. The compositions of solid fermentation medium and culture condition optimization were studied through single factor experiment and orthogonal design. The optimal mediums for the solid-state fermentation of AP-3 production was as follows (w/w):cornstarch, soymeal and cottonseed mass ratio 1:1:1, 4% of brown sugar, 2% of corn steep powder, 1% of CaCl₂. The optimal conditions for solid fermentation were as follows:initial pH 7.5, culture temperature 28℃, solid-liquid ratio (mass ratio) 1:0.8, quality of solid medium 25g (250mL conical flask), inoculum volume 150mL/kg, incubation time 7d. Under the optimal conditions, the yield of AP-3 could reach (26.86±1.87)mg/kg (per kilogram of medium). This study provided a new method and idea for the solid-state fermentation for ansamitocin P-3 production.

Key words: ansamitocin P-3, Actinosynnema pretiosum, fermentation, synthesis, optimization