铜离子与葡萄糖协同补料对球头三型孢菌产赤藓糖醇的影响

doi:10.16085/j.issn.1000-6613.2018-0391

化工进展 ›› 2018, Vol. 37 ›› Issue (12): 4830-4836.DOI: 10.16085/j.issn.1000-6613.2018-0391

铜离子与葡萄糖协同补料对球头三型孢菌产赤藓糖醇的影响

康培, 胡翠英, 鞠鑫, 姚雪梅, 陈佳佳, 郭伟强, 李良智

苏州科技大学化学生物与材料工程学院, 江苏苏州 215009

收稿日期:2018-02-26 修回日期:2018-03-30 出版日期:2018-12-05 发布日期:2018-12-05
通讯作者: 李良智,教授,硕士生导师,研究方向为生物化工。
作者简介:康培(1992-),男,硕士研究生,研究方向为生物催化转化过程工程。E-mail:469167588@qq.com。
基金资助:
国家自然科学基金（21376156，21676173）及江苏高校青蓝工程项目。

Effects of synergistic-feeding fermentation with copper ion and glucose addition on erythritol production by Trichosporonoides oedocephalis

KANG Pei, HU Cuiying, JU Xin, YAO Xuemei, CHEN Jiajia, GUO Weiqiang, LI Liangzhi

School of Chemistry, Biology and Material Engineering, Suzhou University of Science and Technology, Suzhou 215009, Jiangsu, China

Received:2018-02-26 Revised:2018-03-30 Online:2018-12-05 Published:2018-12-05

摘要/Abstract

摘要： 为考察葡萄糖和铜离子盐协同补料发酵对球头三型孢菌产赤藓糖醇的影响，在5L发酵罐中先采用不同浓度的葡萄糖进行分批发酵，然后采用优化的葡萄糖浓度并添加CuSO₄·5H₂O进行发酵研究。结果表明，初始葡萄糖浓度为300g/L的赤藓糖醇产量最大为44.52g/L，其体积生产速率为0.371g/（L·h）、转化率为0.167g/g。在此浓度葡萄糖的基础上添加30mg/L的CuSO₄·5H₂O后，赤藓糖醇产量达到49.62g/L，提高了11.5%。进一步控制总糖浓度为300g/L，且初始浓度为200g/L，分别进行单独补糖和协同补糖与铜离子的补料发酵，结果赤藓糖醇产量分别为47.25g/L和55.31g/L，比初始300g/L的葡萄糖分批发酵分别提高了6.1%和24.2%。特别地，协同补糖与CuSO₄·5H₂O后，赤藓糖还原酶（erythrose reductase，ER）的活性在84h达到最大，为0.152U/mg，比单独补糖时提高了18.8%；通过铜离子盐和葡萄糖的协同补料发酵可显著提高赤藓糖醇的产量，最终使赤藓糖醇产率达到0.461g/（L·h）。

关键词: 赤藓糖醇, 赤藓糖还原酶, 协同补料, 发酵, 球头三型孢菌

Abstract: To investigate the effect of synergistic-feeding fermentation using glucose and Cu²⁺ on erythritol production by Trichosporonoides oedocephalis, the batch fermentation was firstly carried out in 5L fermenter with various initial glucose concentrations. Then, the optimal glucose concentration combining with the CuSO₄·5H₂O addition was used for further fed-batch fermentation. Research results showed that the yield of erythritol reached the highest of 44.52g/L when glucose concentration was 300g/L, corresponding to the volumetric productivity of 0.371g/(L·h) and the ratio of the erythritol yield to glucose of 0.167g/g. Namely, the yield of erythritol was 49.62g/L with 30mg/L CuSO₄·5H₂O addition, which was 11.5% higher than that of the control. When the initial glucose concentration was maintained at 200g/L and the total sugar concentration was controlled at 300g/L, the erythritol yield reached 47.25g/L in the fed-batch fermentation and 55.31g/L in the synergistic fed-batch fermentation, respectively. Compared with the 300g/L glucose batch fermentation, the yield of erythritol increased by 6.1% and 24.2% in the process of fed-batch fermentation, respectively. Especially, the erythrose reductase (ER) activities in synergistic-feeding fermentation was higher than the control. The highest ER activity of 0.152U/mg protein was obtained at 84h of synergistic-feeding fermentation, representing 18.8% increase compared with the glucose-feeding fermentation independently. The results indicated that the yield of erythritol increased significantly when synergistic-feeding fermentation with copper ion salt and glucose, and the volumetric productivity of erythritol reached 0.461g/(L·h).

Key words: erythritol, erythrose reductase, synergistic feeding, fermentation, Trichosporonoides oedocephalis

中图分类号:

Q815

康培, 胡翠英, 鞠鑫, 姚雪梅, 陈佳佳, 郭伟强, 李良智. 铜离子与葡萄糖协同补料对球头三型孢菌产赤藓糖醇的影响[J]. 化工进展, 2018, 37(12): 4830-4836.

KANG Pei, HU Cuiying, JU Xin, YAO Xuemei, CHEN Jiajia, GUO Weiqiang, LI Liangzhi. Effects of synergistic-feeding fermentation with copper ion and glucose addition on erythritol production by Trichosporonoides oedocephalis[J]. Chemical Industry and Engineering Progress, 2018, 37(12): 4830-4836.

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铜离子与葡萄糖协同补料对球头三型孢菌产赤藓糖醇的影响

Effects of synergistic-feeding fermentation with copper ion and glucose addition on erythritol production by Trichosporonoides oedocephalis

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