关键词: 生物工程, 生物能源, 酶, 乙醇, 浓醪发酵

Abstract: High-concentration mash fermentation has such characteristics as high cell density，high product concentration and high production rates. So it is the future aim of ethanol industry. High-concentration mash ethanol fermentation techniques have the advantages of saving process water，improving equipment utilization，and reducing energy consumption. So high-concentration mash ethanol fermentation techniques are an important way of improving ethanol production efficiency. Research on high-concentration mash ethanol fermentation techniques is very important in practical application. This paper reviews research progress of high-concentration mash ethanol fermentation techniques. The definition，advantages and influencing factors of high-concentration mash ethanol fermentation are introduced. Lowering the viscosity of fermentation mash，screening high-tolerance ethanol yeast，changing fermentation process model and adding appropriate enzymes and nutrients are important methods of achieving high-concentration mash ethanol fermentation. Among them，screening high-tolerance ethanol yeast is the key to high-concentration mash fermentation.

Key words: biological engineering, bioenergy, enzyme, ethanol, high-concentration mash fermentation