木材腐朽真菌定向降解木质素为苯系芳香类化学品

化工进展 ›› 2015, Vol. 34 ›› Issue (s1): 173-177.

木材腐朽真菌定向降解木质素为苯系芳香类化学品

秦海蛟, 付云, 文瑞芝, 马强, 胡云楚, 谢练武

中南林业科技大学理学院, 应用化学研究所, 湖南长沙 410004

出版日期:2015-09-20 发布日期:2015-10-20
通讯作者: 谢练武,博士,副教授。从事天然产物化学、微生物降解转化、生物化工等研究。E-mail xiecsu@126.com。
作者简介:秦海蛟(1990—),男,硕士研究生,主要研究木质素结构转化。
基金资助:
国家自然科学基金(31170521),中国博士后科学基金(2013M531787),湖南省科技计划项目(2013RS4035)。

Aromatic benzene derivatives decomposed from lignin by wood decay fungi

QIN Hai, FU Yun, WEN Rui, MA Qiang, HU Yun, XIE Lian

Institute of Applied Chemistry, College of Sciences, Central South University of Forestry and Technology, Changsha 410004, Hunan, China

Online:2015-09-20 Published:2015-10-20

摘要/Abstract

摘要： 对2种木材腐朽真菌降解木质素为一系列苯系芳香类化学品进行了初步研究,并与黄孢原毛平革菌进行了比较。从木材腐朽土壤样品中分离获得具有降解木质素活性的真菌11株,将其中活性最强的菌株ZS1与ZSH用于降解工业碱木质素与中性木质素。通过发酵条件的优化、降解产物种类与浓度的HPLC分析,结果发现,在初始底物浓度1.0～20.0g/L变化时,ZS1对碱木质素的降解率达到40.7%,比ZSH的大;而ZSH对中性木质素的降解率为32.3%,与黄孢原毛平革菌的降解能力相当,但比ZS1的大些。木质素能被定向降解为香草酸、苯甲酸、香草醛、苯酚、紫丁香醛、苯、对甲基苯甲醚、对甲基苯乙醚、甲苯与对二甲苯等芳香类化学品,而且不同真菌降解不同木质素的产物种类数量与浓度有所差异。这说明不同真菌对木质素的降解具有一定的底物依赖性,底物结构差异影响了降解率不同与产物种类差异,而且不同真菌降解木质素具有一定的产物定向选择性。

关键词: 芳香类化学品, 碱木质素, 中性木质素, 降解, 底物依赖性

Abstract: Compared with Phanerochaete chrysosporium Burdsall, a series of benzene aromatic chemicals derived from lignin decomposed by wood decay fungi were investigated.Eleven fungi with potency of degrading lignin were isolated from decay wood samples, and two of them with the highest degradation ability, ZS1 and ZSH, were used to biodegrade alkali lignin and neutral lignin.Through optimizing biodegradation condition and HPLC analysis of degraded products, it was found that, within initial concentrations of 1.0—20.0 g/L, at most 40.7% alkali lignin could be decomposed by ZS1 which is higher than ZSH, while decomposition rate of neutral lignin by ZSH could reach 32.3% which is higher than ZS1.These decomposition rates could be close to that of P.chrysosporium Burdsall.The results also indicated that the lignin could be deconstructed to benzene derivatives chemicals such as vanillic acid, benzoic acid, vanillic aldehyde, phenol, syring aldehyde, benzene, 4-methyl anisole, 4-ethyl anisole, toluene, and p-xylene, which the quantity and concentration of degraded products depended on the type of fungi.The lignin-degrading performance of different fungi showed certain dependency on the structure of substrates, and presented some directional selectivity to products.

Key words: aromatic chemicals, alkali lignin, neutral lignin, decomposition, substrate dependence

秦海蛟, 付云, 文瑞芝, 马强, 胡云楚, 谢练武. 木材腐朽真菌定向降解木质素为苯系芳香类化学品[J]. 化工进展, 2015, 34(s1): 173-177.

QIN Hai, FU Yun, WEN Rui, MA Qiang, HU Yun, XIE Lian. Aromatic benzene derivatives decomposed from lignin by wood decay fungi[J]. Chemical Industry and Engineering Progree, 2015, 34(s1): 173-177.

参考文献

[1] Friedlingstein P, Prentice I C.Carbon-climate feedbacks: a review of model and observation based estimates [J].Current Opinion in Environmental Sustainability, 2010, 2(4): 251-257.

[2] Luque R, Herrero-Davila L, Campelo J M, et al.Biofuels: A technological perspective [J].Energy & Environmental Science, 2008, 1(5): 542-564.

[3] Demirbas A.Competitive liquid biofuels from biomass [J]. Applied Energy, 2011, 88(1): 17-28.

[4] Zakzeski J, Bruijnincx P C A, Jongerius A L, et al.The catalytic valorization of lignin for the production of renewable chemicals [J].Chemical Reviews, 2010, 110(6): 3552-3599.

[5] Stöcker M.Biofuels and biomass-to-liquid fuels in the biorefinery: Catalytic conversion of lignocellulosic biomass using porous materials [J].Angewandte Chemie International Edition, 2008, 47(48): 9200-9211.

[6] 路瑶, 魏贤勇, 宗志敏, 等.木质素的结构研究与应用[J].化学进展, 2013, 25(5): 838-858.

[7] Kotake T, Kawamoto H, Saka S.Pyrolysis reactions of coniferyl alcohol as a model of the primary structure formed during lignin pyrolysis [J].Journal of Analytical and Applied Pyrolysis, 2013, 104(11): 573-584.

[8] Cheng S, Wilks C, Yuan Z, et al.Hydrothermal degradation of alkali lignin to bio-phenolic compounds in sub/supercritical ethanol and water-ethanol co-solvent [J].Polymer Degradation and Stability, 2012, 97(6): 839-848.

[9] 李志礼, 肖多, 葛圆圆.木质素催化热裂解转化为低分子化学品的研究进展[J].中华纸业, 2013, 34(14): 46-49.

[10] Zhao Y, Xu Q, Pan T, et al.Depolymerization of lignin by catalytic oxidation with aqueous polyoxometalates [J]. Applied Catalysis A: General, 2013, 467: 504-508.

[11] Amen-Chen C, Pakdel H, Roy C.Production of monomeric phenols by thermochemical conversion of biomass: A review [J].Bioresource Technology, 2001, 79(3): 277-299.

[12] Chang Y C, Choi D, Takamizawa K, et al.Isolation of Bacillus sp.strains capable of decomposing alkali lignin and their application in combination with lactic acid bacteria for enhancing cellulase performance [J].Bioresource Technology, 2014, 152(1): 429-436.

[13] 张辉, 戴传超, 朱奇, 等.生物降解木质素研究新进展[J].安徽农业科学, 2006, 34(9): 1780-1784.

[14] Brown M E, Chang M C Y.Exploring bacterial lignin degradation [J].Current Opinion in Chemical Biology, 2014, 19: 1-7.

[15] 郁红艳, 曾光明, 牛承岗, 等.细菌降解木质素的研究进展[J].环境科学与技术, 2005, 28(2): 104-106,109.