关键词: 解脂耶氏酵母, 苯乙酮, (R)-苯乙醇, 生物催化, 酶, 优化

Abstract: In order to improve Yarrowia lipolytica's catalytic efficiency for acetophenone ,using 20mmol/L acetophenone and 20mmol/L phenyl ethanol as screening pressure,we obtained a mutant strain through UV mutation. Then we optimized the reaction conditions,such as temperature,pH,co-substrate and organic solvents. The mutant strain Yarrowia lipolytica ACA-DC 50109-H could reduce 20mmol/L acetophenone to optical pure (R)-phenyl ethanol (76% conversion,99.2% e.e.). The optimal reaction conditions were using glucose as co-substrate,and with t=30℃,pH=7.0 and the addition of 30%(v/v) ionic liquid (BMIM·PF6). When the culture broth of Yarrowia lipolytica ACA-DC 50109-H was used as the reaction system,with the addition of 30% BMIM·PF6, 60mmol/L acetophenone could be reduced to (R)-phenyl ethanol (97.5% conversion,95% e.e.) after 36h. Yarrowia lipolytica ACA-DC 50109-H could efficiently catalyze the asymmetric reduction of α-acetophenone to (R)-phenyl ethanol with high substrate loading,high conversion and e.e..

Key words: Yarrowia lipolytica, acetophenone, (R)-phenyl ethanol, biocatalysis, enzyme, optimization