关键词: Clostridium saccharobutylicum；连续发酵；丁醇；稀释率, 温度

Abstract: To provide theoretical basis and experiment guidance for continuous biobutanol fermentation using cheap sugar substrate, the effects of dilution rate and temperature on continuous biobutanol fermentation by Clostridium saccharobutylicum DSM 13864 using glucose as substrate were investigated. The results showed that acid accumulation and cell density were enhanced at higher dilution rates, while solvent production was improved at lower dilution rates. At dilution rate of 0.05 h?1 and 37℃, total solvent concentration of 11.57 g/L (7.29 g/L butanol) and solvent productivity of 0.145 g/(L?h) were reached. The continuous fermentation process using temperature-shifting strategy demonstrated that a lower temperature was beneficial to solvent accumulation. When fermentation temperatures of the first two stages (Ⅰ & Ⅱ) and the later two stages (Ⅲ & Ⅳ) were controlled at 37 ℃ and 33 ℃ respectively, total solvent concentration of 13.69 g/L (8.36 g/L butanol) and solvent productivity of 0.171 g/(L?h) were achieved.

Key words: Clostridium saccharobutylicum, continuous fermentation, butanol, dilution rate, temperature