关键词: 产氢菌, 16S rDNA, 制氢, 发酵, 协同作用

Abstract: In order to obtain high performance hydrogen-producing bacteria, two strains (H-1 and H-2) were screened from activated sludge which was pretreated by alkali. Biological identification showed that the two strains were of Enterobacter species. H-1 was Enterobacter cancerogeous HG6 2A species and H-2 had the closest relationship with Enterobacter homaechei83. Results showed that the best performance could be achieved from co-fermentation at the ratio of 1:1 (H-1:H-2). The corresponding fermentation time and hydrogen yield were 33h and 861mL/L, respectively. The defects of lower hydrogen yield by H-1 and longer fermentation time by H-2 were overcome by co-fermentation. The main volatile fatty acids (VFA) in the broth were acetate and butyrate, indicating that the fermentation of the two strains were both butyrate type. Due to the synergistic effect of the two strains, the accumulation rate of VFA reduced at the initial of fermentation. As a result, the stability of the system was enhanced.

Key words: hydrogenogen, 16S rDNA, hydrogen production, fermentation, synergistic effect