H2S气态基质对污泥生物沥滤处理效能的影响

doi:10.16085/j.issn.1000-6613.2022-1348

化工进展 ›› 2023, Vol. 42 ›› Issue (5): 2733-2743.DOI: 10.16085/j.issn.1000-6613.2022-1348

H₂S气态基质对污泥生物沥滤处理效能的影响

常占坤¹^,²(), 张弛¹(), 苏冰琴¹^,², 张聪政³, 王健¹^,², 权晓慧¹^,²

^1.太原理工大学环境科学与工程学院，山西太原 030024
^2.山西省市政工程研究生教育创新中心，山西太原 030024
^3.山西省安装集团股份有限公司，山西太原 030024

收稿日期:2022-07-19 修回日期:2022-09-18 出版日期:2023-05-10 发布日期:2023-06-02
通讯作者: 张弛
作者简介:常占坤（1997—），女，硕士研究生，研究方向为固体废物处理与处置。E-mail：changzhankun0325@163.com。
基金资助:
山西省社会发展科技攻关计划(201803D31039);山西省国际科技合作计划(201803D421098)

Effect of H₂S gaseous substrate on sludge bioleaching treatment efficiency

CHANG Zhankun¹^,²(), ZHANG Chi¹(), SU Bingqin¹^,², ZHANG Congzheng³, WANG Jian¹^,², QUAN Xiaohui¹^,²

^1.School of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China
^2.Municipal Engineering Graduate Education Innovation Center of Shanxi Province, Taiyuan 030024, Shanxi, China
^3.Shanxi Province Installation Group Co. , Ltd. , Taiyuan 030024, Shanxi, China

Received:2022-07-19 Revised:2022-09-18 Online:2023-05-10 Published:2023-06-02
Contact: ZHANG Chi

摘要/Abstract

摘要：

采用H₂S作为污泥生物沥滤的部分替代营养底物，探究了不同的H₂S负荷对生物沥滤处理效能和群落动态的影响。实验考察了生物沥滤过程中pH、ORP、重金属去除率及CST的变化，并进行了群落多样性分析。结果表明：通入气体流量为2mL/min、负荷50mL/L的H₂S，能显著加快生物沥滤进程，提高污泥体系中pH的下降速率，保持更高的氧化还原电位。H₂S的加入不仅改善了脱水性能，CST加快了35.8s，而且对重金属的浸出也有显著的效果，Ni、Pb、Cr的去除率分别提高了18.35%、21.22%、13.07%，同时促进了各种重金属形态向易溶态的转化。电镜结果显示H₂S的加入促进了优势菌群繁殖，并使污泥颗粒变大且紧实。高通量测序显示，H₂S气态基质的加入强化了污泥生物沥滤处理效能，加速了污泥体系中的物种数、菌群丰度、群落多样性的减小趋势，促进变形菌门成为优势菌门，嗜酸菌属成为优势菌属。

关键词: H₂S, 生物沥滤, 污泥处理, 重金属, 脱水性能, 群落多样性

Abstract:

H₂S was used as the partially alternative nutrient substrate for sludge bioleaching, and the effects of H₂S load on bioleaching treatment efficiency and community diversity were studied. The changes of pH, ORP, heavy metal removal rate and CST were examined, and the community diversity was analyzed. The results showed that H₂S load of 50mL/L with gas flow of 2mL/min could significantly accelerate the bioleaching process, improve the decline rate of pH and maintain a higher oxidation-reduction potential in the sludge system. The addition of H₂S improved dewaterability with CST shortened by 35.8s, had significant effects on heavy metal leaching with the removal rate of Ni, Pb, and Cr increasing by 18.35%, 21.22%, and 13.07% respectively, and promoted the conversion of various heavy metal forms to the easily soluble state. The SEM results showed that H₂S promoted the reproduction of dominant microflora and made the sludge particles larger and compact. High-throughput sequencing showed that the addition of H₂S gaseous substrate strengthened the efficiency of sludge bioleaching treatment, accelerated the trend reducing the number of species, microflora abundance and community diversity in the sludge system, and promoted Proteobacteria as the dominant phylum and Acidithiobacillu as the dominant genus.

Key words: hydrogen sulfide, bioleaching, sludge treatment, heavy metal, dewaterability, community diversity

中图分类号:

X705

常占坤, 张弛, 苏冰琴, 张聪政, 王健, 权晓慧. H₂S气态基质对污泥生物沥滤处理效能的影响[J]. 化工进展, 2023, 42(5): 2733-2743.

CHANG Zhankun, ZHANG Chi, SU Bingqin, ZHANG Congzheng, WANG Jian, QUAN Xiaohui. Effect of H₂S gaseous substrate on sludge bioleaching treatment efficiency[J]. Chemical Industry and Engineering Progress, 2023, 42(5): 2733-2743.

图/表 13

图1 不同H2S负荷下污泥生物沥滤过程中pH及完成度的变化情况

图2 H2S负荷50mL/L组与不同营养底物效果对比

图3 不同H2S负荷下污泥生物沥滤过程中ORP的变化情况

图4 不同H2S负荷下污泥生物沥滤过程中Ni、Pb、Cr去除率的变化情况

图5 H2S负荷50mL/L组与对照组的Ni、Pb、Cr去除率随ORP值的变化关系

图6 通H2S前后重金属赋存形态的变化情况

图7 不同H2S负荷下污泥生物沥滤过程中CST的变化情况

图8 污泥生物沥滤过程中H2S负荷50mL/L与对照组的SEM对比图（放大2000倍）

表1 菌群Alpha多样性指数表

分组	观察到的物种数	覆盖度指数	Chao1	Simpson 指数	Shannon 指数	Pieloue 指数
O1	5378.6	0.992	5405.77	0.987	9.974	0.805
B1	4693.1	0.990	4754.35	0.985	9.227	0.757
F1	2775.1	0.991	2884.43	0.939	7.077	0.619
B2	4603.2	0.990	4715.9	0.976	8.941	0.735
F2	620.3	0.998	637.566	0.857	4.614	0.497

图9 不同样本ASV/OTU的韦恩图[图中每个圈代表一个样本，圈和圈重叠部分的数字代表样本之间共有的OTUs个数，没有重叠部分的数字代表样本特有的OTUs个数]

图10 门水平微生物群落结构

图11 属水平微生物群落结构

图12 24h内H2S去除率的变化

参考文献 32

1	边耀至, 郑雄. 城市污水处理厂污泥的处理与处置分析[J]. 环境与发展, 2019, 31(1): 84, 86.
	BIAN Yaozhi, ZHENG Xiong. Analysis of treatment and disposal of sludge from municipal wastewater treatment plant[J]. Environment and Development, 2019, 31(1): 84, 86.
2	YANG Guang, ZHANG Guangming, WANG Hongchen. Current state of sludge production, management, treatment and disposal in China[J]. Water Research, 2015, 78: 60-73.
3	ZHANG Q H, YANG WN, NGO H H, et al. Current status of urban wastewater treatment plants in China[J]. Environment International, 2016, 92/93: 11-22.
4	XIONG Qiao, ZHOU Min, LIU Mengjia, et al. The transformation behaviors of heavy metals and dewaterability of sewage sludge during the dual conditioning with Fe²⁺-sodium persulfate oxidation and rice husk[J]. Chemosphere, 2018, 208: 93-100.
5	郑金鑫, 邱春生, 王晨晨, 等. Fenton处理对污泥脱水性, 重金属形态及生物淋滤效率影响[J]. 化工进展, 2020, 39(2): 805-811.
	ZHENG Jinxin, QIU Chunsheng, WANG Chenchen, et al. Effects of Fenton treatment on sewage sludge dewaterability, heavy metal speciation and leaching efficiency[J]. Chemical Industry and Engineering Progress, 2020, 39(2): 805-811.
6	HUANG Jinjia, LIANG Jialin, YANG Xian, et al. Ultrasonic coupled bioleaching pretreatment for enhancing sewage sludge dewatering: Simultaneously mitigating antibiotic resistant genes and changing microbial communities[J]. Ecotoxicology and Environmental Safety, 2020, 193: 110349.
7	WONG J C, XIANG L, CHAN L C. pH requirement for the bioleaching of heavy metals from anaerobically digested wastewater sludge[J]. Water, Air and Soil Pollution, 2002, 138(1): 25-35.
8	刘奋武, 周立祥. 不同能源物质配合及化学强化对生物沥浸法提高城市污泥脱水性能的效果[J]. 环境科学学报, 2009, 29(5): 974-979.
	LIU Fenwu, ZHOU Lixiang. Enhancing dewaterability of municipal sewage sludge through the combined approaches of bioleaching and Fenton reaction[J]. Acta Scientiae Circumstantiae, 2009, 29(5): 974-979.
9	吴建华, 邱信欣, 刘锋, 等. 生物滴滤塔处理硫化氢废气[J]. 化工环保, 2019, 39(3): 278-282.
	WU Jianhua, QIU Xinxin, LIU Feng, et al. Treatment of H₂S-containing waste gas in bio-trickling filter[J]. Environmental Protection of Chemical Industry, 2019, 39(3): 278-282.
10	李李, 曹煜, 林璠, 等. 国内外处理硫化氢的研究现状[J]. 广州化工, 2015, 43(8): 27-29.
	LI Li, CAO Yu, LIN Fan, et al.Current research deal with hydrogen sulfide[J].Guangzhou Chemical Industry, 2015, 43(8): 27-29.
11	MOUNA B J, COUVERT A, AMRANE A, et al. Biofiltration of high concentration of H₂S in waste air under extreme acidic conditions[J]. New Biotechnology, 2016, 33(1): 136-143.
12	JABER M B, ANET B, AMRANE A, et al. Impact of nutrients supply and pH changes on the elimination of hydrogen sulfide, dimethyl disulfide and ethanethiol by biofiltration[J]. Chemical Engineering Journal, 2014, 258: 420-426.
13	叶姜瑜, 沈秀红, 王琳, 等. Bio-SR工艺去除硫化氢气体的研究[J]. 环境工程学报, 2012, 6(2): 584-588.
	YE Jiangyu, SHEN Xiuhong, WANG Lin, et al. Research on H₂S removal by Bio-SR process[J]. Chinese Journal of Environmental Engineering, 2012, 6(2): 584-588.
14	孙事昊, 贾体沛, 陈凯琦, 等. 聚丙烯环生物滴滤塔去除实际市政污水厂硫化氢性能及微生物群落分析[J]. 环境科学, 2019, 40(10): 4585-4593.
	SUN Shihao, JIA Tipei, CHEN Kaiqi, et al. Removal of hydrogen sulfide produced in a municipal WWTP using a biotrickling filter with polypropylene rings as the packing material and microbial community analysis[J]. Environmental Science, 2019, 40(10): 4585-4593.
15	许海朋, 李岩, 牧辉, 等. 排硫硫杆菌净化处理沼气中H₂S实验研究[J]. 中国沼气, 2019, 37(2): 3-6.
	XU Haipeng, LI Yan, MU Hui, et al. Removal of H₂S from biogas by tiobacillus thioparus immobilized on granular active carbon[J]. China Biogas, 2019, 37(2): 3-6.
16	田维平, 陈学民, 周鹏, 等. 蚯蚓粪净化硫化氢恶臭气体[J]. 环境工程学报, 2018, 12(11): 3169-3176.
	TIAN Weiping, CHEN Xuemin, ZHOU Peng, et al. Removal of hydrogen sulfide contaminated air by using vermicompost mediated bioreactor[J]. Chinese Journal of Environmental Engineering, 2018, 12(11): 3169-3176.
17	LIU Fenwu, ZHOU Lixiang, ZHOU Jun, et al. Improvement of sludge dewaterability and removal of sludge-borne metals by bioleaching at optimum pH[J]. Journal of Hazardous Materials, 2012, 221/222: 170-177.
18	冯素萍, 刘慎坦, 杜伟, 等. 利用BCR改进法和Tessier修正法提取不同类型土壤中Cu、Zn、Fe、Mn的对比研究[J]. 分析测试学报, 2009, 28(3): 297-300.
	FENG Suping, LIU Shentan, DU Wei, et al. Assessment of Cu, Zn, Fe, Mn species in different soils by modified BCR and tessie rextraction procedures[J]. Journal of Instrumental Analysis, 2009, 28(3): 297-300.
19	李洋. 小分子有机酸对氧化亚铁硫杆菌的抑制作用及其机理的初步研究[D]. 南京: 南京农业大学, 2010.
	LI Yang. Mechanisms involved in lmwoas-caused inhibition on ferrous iron oxidation by acidithiobacillus ferrooxidans[D]. Nanjing: Nanjing Agricultural University, 2010.
20	王庭. 硫化物生物氧化为单质硫的研究[D]. 无锡: 江南大学, 2008.
	WANG Ting. The study of sulfide bio-oxidation to sulfur[D]. Wuxi: Jiangnan University, 2008.
21	周思宇, 孙水裕, 廖小健, 等. Sulfobacillus benefaciens强化中度嗜热菌群浸出铅锌硫化矿尾矿过程机制[J]. 环境科学学报, 2021, 41(11): 4648-4659.
	ZHOU Siyu, SUN Shuiyu, LIAO Xiaojian, et al. Mechanism of Sulfobacillus benefaciens enhancing the lead-zinc sulfide tailings bioleaching performance by moderate thermophilic consortia[J]. Acta Scientiae Circumstantiae, 2021, 41(11): 4648-4659.
22	FONTMORIN J M, SILLANPÄÄ M. Bioleaching and combined bioleaching/Fenton-like processes for the treatment of urban anaerobically digested sludge: Removal of heavy metals and improvement of the sludge dewaterability[J]. Separation and Purification Technology, 2015, 156: 655-664.
23	张弛, 苏冰琴, 李超, 等. 气态硫基质对污泥生物沥滤的促进效应研究[J]. 太原理工大学学报, 2020, 51(6): 823-830.
	ZHANG Chi, SU Bingqin, LI Chao, et al. Study on promoting effect of gaseous sulfur substrate on sludge bioleaching[J]. Journal of Taiyuan University of Technology, 2020, 51(6): 823-830.
24	GAN Min, ZHOU Shuang, LI Mingming, et al. Bioleaching of multiple heavy metals from contaminated sediment by mesophile consortium[J]. Environmental Science and Pollution Research, 2015, 22(8): 5807-5816.
25	华玉妹, 李文红, 陈英旭, 等. 不同接种量下生物沥滤去除污泥中重金属的研究[J]. 浙江大学学报(农业与生命科学版), 2005, 31(1): 47-51.
	HUA Yumei, LI Wenhong, CHEN Yingxu, et al. Effect of amount of inoculum on the bioleaching of heavy metals from sewage sludge[J]. Journal of Zhejiang University (Agric& Life Sci), 2005, 31(1): 47-51.
26	祁丽, 梅竹松, 邱春生, 等. 生物淋滤对城市污泥重金属去除及形态变化的影响研究[J]. 环境污染与防治, 2018, 40(10): 1116-1121.
	QI Li, MEI Zhusong, QIU Chunsheng, et al. Effects of bioleaching on removal and morphological changes of heavy metals in sewage sludge[J]. Environmental Pollution & Control, 2018, 40(10): 1116-1121.
27	YANG Wenfeng, ZENG Liyuan, ZHANG Weihao, et al. The influence of different sludge concentrations on its dewaterability during bioleaching[J]. Water Science and Technology, 2020, 81(12): 2585-2598.
28	DENEUX-MUSTIN S, LARTIGES B S, VILLEMIN G, et al. Ferric chloride and lime conditioning of activated sludges: An electron microscopic study on resin-embedded samples[J]. Water Research, 2001, 35(12): 3018-3024.
29	LI Bing, ZHANG Xuxiang, GUO Feng, et al. Characterization of tetracycline resistant bacterial community in saline activated sludge using batch stress incubation with high-throughput sequencing analysis[J]. Water Research, 2013, 47(13): 4207-4216.
30	张堃, 徐颖, 周媛, 等. 砷尾矿污染土壤的细菌群落结构多样性及其相关环境影响因子分析[J]. 生态环境学报, 2021, 30(2): 391-399.
	ZHANG Kun, XU Ying, ZHOU Yuan, et al. Analysis of microbial community structure and environmental impact factors of arsenic mine tailings[J]. Ecology and Environmental Sciences, 2021, 30(2): 391-399.
31	夏芳芳. 垃圾生物覆盖土对填埋气中H₂S的净化作用及机理研究[D]. 杭州: 浙江大学, 2014.
	XIA Fangfang. H₂S removal from landfill gas and its mechanism in waste biocover soil[D]. Hangzhou: Zhejiang University, 2014.
32	解道雷, 孔慈明, 徐龙乾, 等. 城市污泥中重金属存在形态、去除及稳定化研究进展[J]. 化工进展, 2018, 37(1): 330-342.
	XIE Daolei, KONG Ciming, XU Longqian, et al. Developments of the speciation,removal and stabilization of heavy metals in municipal sludge[J]. Chemical Industry and Engineering Progress, 2018, 37(1): 330-342.

[1]	许春树, 姚庆达, 梁永贤, 周华龙. 共价有机框架材料功能化策略及其对Hg(Ⅱ)和Cr(Ⅵ)的吸附性能研究进展[J]. 化工进展, 2023, 42(S1): 461-478.
[2]	李世霖, 胡景泽, 王毅霖, 王庆吉, 邵磊. 电渗析分离提取高值组分的研究进展[J]. 化工进展, 2023, 42(S1): 420-429.
[3]	李志远, 黄亚继, 赵佳琪, 于梦竹, 朱志成, 程好强, 时浩, 王圣. 污泥与聚氯乙烯共热解重金属特性[J]. 化工进展, 2023, 42(9): 4947-4956.
[4]	李卫华, 于倩雯, 尹俊权, 吴寅凯, 孙英杰, 王琰, 王华伟, 杨玉飞, 龙於洋, 黄启飞, 葛燕辰, 何依洋, 赵灵燕. 酸雨环境下填埋飞灰吨袋破损后重金属的溶出行为[J]. 化工进展, 2023, 42(9): 4917-4928.
[5]	张杉, 仲兆平, 杨宇轩, 杜浩然, 李骞. 磷酸盐改性高岭土对生活垃圾热解过程中重金属的富集[J]. 化工进展, 2023, 42(7): 3893-3903.
[6]	李若琳, 何少林, 苑宏英, 刘伯约, 纪冬丽, 宋阳, 刘博, 余绩庆, 徐英俊. 原位热解对油页岩物性及地下水水质影响探索[J]. 化工进展, 2023, 42(6): 3309-3318.
[7]	庄捷, 薛锦辉, 赵斌成, 张文艺. 猪粪厌氧消化进程中重金属与腐殖质的有机结合机制[J]. 化工进展, 2023, 42(6): 3281-3291.
[8]	郑昕, 贾里, 王彦霖, 张靖超, 陈世虎, 乔晓磊, 樊保国. 污泥与煤泥混烧对重金属固留特性的影响[J]. 化工进展, 2023, 42(6): 3233-3241.
[9]	李卫华, 吴寅凯, 孙英杰, 尹俊权, 辛明学, 赵友杰. 垃圾焚烧飞灰重金属毒性浸出评价方法研究进展[J]. 化工进展, 2023, 42(5): 2666-2677.
[10]	郭宇晨, 刘庆林, 蒋金洋, 宗永忠, 王金伟, 李臻, 吕树祥. 含铬污泥资源化方法研究进展[J]. 化工进展, 2023, 42(2): 575-584.
[11]	王玉, 余广炜, 林佳佳, 黎长江, 江汝清, 邢贞娇, 余铖. 沼渣、飞灰和污泥生物炭制备建筑陶粒[J]. 化工进展, 2023, 42(2): 1039-1050.
[12]	李晶晶, 赵曜, 徐沣驰, 李康建. 不同径流冲刷作用下多孔炉渣沥青混合料重金属的浸出特性[J]. 化工进展, 2023, 42(10): 5520-5530.
[13]	唐朝春, 王顺藤, 黄从新, 冯文涛, 阮以宣, 史纯菁. 介孔金属有机框架材料吸附水中重金属离子研究进展[J]. 化工进展, 2022, 41(6): 3263-3278.
[14]	付杰, 邱春生, 王晨晨, 郑金鑫, 刘楠楠, 王栋, 王少坡, 孙力平. 污泥热水解处理过程重金属的迁移转化与风险评价[J]. 化工进展, 2022, 41(4): 2216-2225.
[15]	陈运涛, 董晓旭, 王洋, 王健男, 崔美, 黄仁亮. 巯基化Zr-MOFs/聚酯无纺布复合材料制备及应用[J]. 化工进展, 2022, 41(2): 854-861.

H₂S气态基质对污泥生物沥滤处理效能的影响

Effect of H₂S gaseous substrate on sludge bioleaching treatment efficiency

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 13

参考文献 32

相关文章 15

编辑推荐

Metrics

本文评价