MICP矿化产物中钙离子利用率的影响因素及微观物相分析

doi:10.16085/j.issn.1000-6613.272018-1537

化工进展 ›› 2019, Vol. 38 ›› Issue (05): 2306-2313.DOI: 10.16085/j.issn.1000-6613.272018-1537

MICP矿化产物中钙离子利用率的影响因素及微观物相分析

段宇¹(),徐国宾¹,杨德锋²,闫玥¹()

1. 天津大学水利工程仿真与安全国家重点实验室，天津300354
2. 河北工程大学水利水电学院，河北邯郸056038

收稿日期:2018-07-24 修回日期:2018-12-16 出版日期:2019-05-05 发布日期:2019-05-05
通讯作者: 闫玥
作者简介:<named-content content-type="corresp-name">段宇</named-content>(1990—)，男，博士研究生，研究方向为新型微生物建筑材料。E-mail：<email>duan_09@126.com</email>。
基金资助:
国家自然科学基金(51709198);天津市自然科学基金(16JCQNJC07900)

Influencing factors of calcium ion utilization in MICP mineralized products and analysis of microscopic image

Yu DUAN¹(),Guobin XU¹,Defeng YANG²,Yue YAN¹()

1. State Key Laboratory of Hydraulic Engineering Simulation and Safety, Tianjin University, Tianjin 300354, China
2. Shool of Water Conservancy and Hydroelectric Power, Hebei University of Engineering, Handan 056038, Hebei, China

Received:2018-07-24 Revised:2018-12-16 Online:2019-05-05 Published:2019-05-05
Contact: Yue YAN

摘要/Abstract

摘要：

钙离子利用率是微生物诱导碳酸钙沉积矿化技术中一项重要指标和参数，待矿化钙离子能否参与到矿化反应和如何被利用是这项技术的关键。本文借助紫外线吸光度法、电导率法和EDTA滴定法等技术手段，分析了待胶结菌液浓度和脲酶活性的时变规律，阐述了不同胶结配比对矿化反应过程中钙离子利用率的影响。结果表明：在胶结过程中，细菌的浓度和脲酶活性会逐渐降低；在合理浓度范围内，钙离子利用率随菌液浓度以及胶结液浓度的增大而提高，最高可达99.73%。进而通过X射线衍射、扫描电镜检测来揭示矿化产物的形成机理，分析得出：球霰状碳酸钙晶体是钙离子在有机质的调控下依托细菌表面的成核位点富集矿化而成，矿化产物中碳酸钙晶体尺寸大小和形态受菌液和胶结液配比浓度的影响。本研究对于微生物诱导矿化反应生成碳酸钙在工程材料领域的应用具有一定的参考价值。

关键词: 微生物诱导碳酸钙矿化沉淀技术, 钙离子利用率, 时变规律, 微观结构, 物相分析

Abstract:

The utilization of calcium ions is an important index and parameter in microbially induced calcium carbonate deposition. The key to this technology is whether it can participate in the mineralization reaction. In this study, UV absorbance, conductivity measurement and EDTA titration were used to analyze the time-varying law of bacterium concentration and urease activity, and also the effects of different cementing ratios on the utilization of calcium ion in the mineralization process were discussed. The results showed that, the concentration and urease activity of the bacteria gradually decreased during the cementing process. Within a proper concentration range, calcium ion utilization increased with the increase of bacteria concentration and cementation concentration, and the highest value was 99.73%. The formation mechanism of mineralization products was revealed by X-ray diffraction and scanning electron microscopy. The analysis showed that the sphere-like calcium carbonate crystals are formed by the enrichment and mineralization of calcium ions at the nucleation sites on the surface of the bacteria under the control of organic matter. The size and morphology of the calcium carbonate crystals are affected by the concentration ratio of the bacterial liquid and the cement liquid. This research has particular reference value for the application of calcium carbonate in mineralization induced by microorganisms and its application in engineering materials.

Key words: microbial induced carbonate precipitation (MICP), utilization rate of calcium ion, time-dependent deformation, microstructure, phase analysis

中图分类号:

TU59

段宇, 徐国宾, 杨德锋, 闫玥. MICP矿化产物中钙离子利用率的影响因素及微观物相分析[J]. 化工进展, 2019, 38(05): 2306-2313.

Yu DUAN, Guobin XU, Defeng YANG, Yue YAN. Influencing factors of calcium ion utilization in MICP mineralized products and analysis of microscopic image[J]. Chemical Industry and Engineering Progress, 2019, 38(05): 2306-2313.

图/表 9

图1 微生物生长特性曲线

图2 实验流程图

图3 不同浓度菌液OD600值时变图

图4 不同浓度菌液自然静置脲酶活性变化图

图5 矿化反应中白色絮状物沉淀

图6 不同配比微生物矿化反应钙离子变化图

图7 矿化产物XRD谱图

图8 溶液内矿化产物SEM扫描电镜图

图9 高倍镜下碳酸钙晶体SEM扫描电镜图

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MICP矿化产物中钙离子利用率的影响因素及微观物相分析

Influencing factors of calcium ion utilization in MICP mineralized products and analysis of microscopic image

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