关键词: 赖氨酸接枝海藻酸盐, 微胶珠, 正交设计, 优化

Abstract: A new material，ALG-g-Lys，was used to prepare micro-beads instead of alginate to improve the stability of alginate micro-beads. The preparation process was optimized using the single factor method combined with orthogonal design. The optimized parameters were：ALG-g-Lys concentration 1.5g/L，voltage 8.0kV，advance speed 30mm/h，needle 24G，and CaCl2 concentration 15g/L. The prepared micro-beads showed even distribution and good sphericity with low trailing ratio，half sphere ratio and globule ratio. The mean particle size was 253±40μm. This work supplies a novel carrier material instead of alginate to prepare gel，showing bright future in medicine and food industries.

Key words: lysine grafted alginate, micro-bead, orthogonal design, optimization