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响应面法优化甘露醇产丁醇的发酵条件

李 锋1,2,温顺华1,黄庶冰3,吴卫红2,廖 威4,王何健5,黄庶识1   

  1. 1广西科学院生物物理实验室,广西 南宁 530007;2广西科技大学生物与化学工程学院,广西 柳州 545006; 3广西大学商学院,广西 南宁 530004;4广西职业技术学院食品与生物技术系,广西 南宁 530226;5广西科学院国家非粮生物质能源工程技术研究中心,广西 南宁 530007
  • 出版日期:2013-07-05 发布日期:2013-07-05

Optimization of butanol production from mannitol by Clostridium acetobutylicum ATCC824 using response surface method

LI Feng 1,2,WEN Shunhua 1,HUANG Shubing3,WU Weihong 2,LIAO Wei 4,WANG Hejian5,HUANG Shushi 1   

  1. 1Lab of Biophysics,Guangxi Academy of Sciences,Nanning 530007,Guangxi,China;2 School of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,Guangxi,China;3Business School,Guangxi University,Nanning 530004,Guangxi,China;4 Department of Food and Biotechnology,Guangxi Vocational & Technical College,Nanning 530226,Guangxi,China;5National Engineering Research Center for non-food Biorefinery,Guangxi Academy of Sciences,Nanning 530007,Guangxi,China
  • Online:2013-07-05 Published:2013-07-05

摘要: 以褐藻的主要成分甘露醇为底物,利用丙酮丁醇梭菌ATCC824发酵生产丁醇。采用Plackett-Burman(P-B)和Box-Behnken优化发酵条件。在Plackett-Burman实验设计时,考察牛肉膏、酵母浸粉、胰蛋白胨、乙酸铵、 KH2PO4、MgSO4?7H2O、FeSO4?7H2O、接种量、发酵温度、初始pH值这10个因素对丁醇产量影响,筛选出影响丁醇发酵的重要参数是发酵温度、初始pH值、乙酸铵浓度,利用Box-Behnken设计确定最优发酵条件为发酵温度37.3 ℃、初始pH值6.38、乙酸铵浓度2.82 g/L。数学模型分析预测最大丁醇产量为8.47 g/L。经实验验证表明,在优化条件下的丁醇产量平均值达到8.52±0.55 g/L。这说明利用统计学方法优化甘露醇的丁醇发酵条件是有效的。

关键词: 甘露醇, 丁醇, Plackett-Burman, Box-Behnken, 丙酮丁醇梭菌, 甘露醇, 丁醇, Plackett-Burman, Box-Behnken, 丙酮丁醇梭菌

Abstract: Mannitol,which is the main component of brown algae,was utilized in this study to produce butanol by Clostridium acetobutylicum (ATCC 824). Plackett-Burman (P-B) and Box-Behnken designs were adopted to optimize the fermentation conditions. Ten factors,including beef extract,yeast extract,tryptone,ammonium acetate,KH2PO4,MgSO4?7H2O,FeSO4?7H2O,size of inoculum,fermentation temperature and initial pH,were considered to affect butanol production in the Plackett-Burman (P-B) design. The result demonstrated that fermentation temperature,initial pH and concentration of ammonium acetate were the most critical factors,and optimal fermentation conditions were 37.3℃,pH 6.38 and 2.82 g/L of ammonium acetate according to the Box-Behnken design. A mathematical model was established and used to predict the maximum butanol yield of 8.47 g/L. The result of verification experiment showed that the maximum butanol yield reached 8.52±0.55g/L under the optimum conditions,which showed that the statistical method was a useful tool for butanol production from mannitol in the optimization.

Key words: mannitol, butanol , Plackett-Burman, Clostridium acetobutylicum , Box-Behnken, mannitol, butanol, Plackett-Burman design, Box-Behnken design, Clostridium acetobutylicum

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