共生菌系TSH06连续发酵生产丁醇

doi:10.16085/j.issn.1000-6613.2017.04.035

化工进展 ›› 2017, Vol. 36 ›› Issue (04): 1418-1423.DOI: 10.16085/j.issn.1000-6613.2017.04.035

共生菌系TSH06连续发酵生产丁醇

李冬月^1,2, 李祥龙², 林樟楠¹, 刘宏娟¹, 张建安¹

1 清华大学核能与新能源技术研究院, 北京 100084;
2 昆明理工大学国土资源工程学院, 云南昆明 650093

收稿日期:2016-09-21 修回日期:2016-10-20 出版日期:2017-04-05 发布日期:2017-04-05
通讯作者: 张建安,副研究员。E-mail:zhangja@tsinghua.edu.cn;刘宏娟,副研究员。E-mail:liuhongjuan@tsinghua.edu.cn。
作者简介:李冬月(1989-),男,硕士研究生。
基金资助:
工业生物催化教育部重点实验室（清华大学）开放基金（2015301）及工业生物催化教育部重点实验室（清华大学）开放基金（2015302）项目。

The continuous butanol fermentation of symbiotic system TSH06

LI Dongyue^1,2, LI Xianglong², LIN Zhangnan¹, LIU Hongjuan¹, ZHANG Jian'an¹

1 Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China;
2 Faculty of Land Resources Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan, China

Received:2016-09-21 Revised:2016-10-20 Online:2017-04-05 Published:2017-04-05

摘要/Abstract

摘要： 传统的产丁醇生产菌均为严格厌氧，本实验室分离出一株兼性厌氧共生菌系TSH06，可在微氧条件下发酵生产丁醇。本文研究了共生菌系TSH06微氧条件下的丁醇连续发酵，考察了稀释率对TSH06细胞生长及丁醇合成的影响。结果表明，低稀释率有利于丁醇的生成，高稀释率有利于酸的积累，丁醇浓度随着稀释率的增大而减小，而生产强度随稀释率的增大而增大。当稀释率大于0.2h^-1时，生产强度呈下降趋势。最优化的稀释率为0.2h^-1，丁醇生产强度可达1.06g/（L·h）。研究结果可为微氧条件下丁醇连续发酵的进一步优化提供理论依据。

关键词: 丁醇, 微氧发酵, 连续发酵, 稀释率, 生产强度

Abstract: The traditional butanol producing strain was strict anaerobic bacteria. A facultative anaerobic symbiotic system TSH06 was isolated in our lab,which could produce butanol under micro-aerobic conditions. This work studied the continuous butanol fermentation of TSH06 under the micro-aerobic condition. The effect of dilution rate on the cell growth and butanol production was investigeted. The test results indicated that the lower dilution rate enhanced the butanol production and the higher dilution rate was beneficial for the acid accumulation. However,the butanol productivity decreased when the dilution rate was higher than 0.2h^-1. The optimized dilution rate was 0.2h^-1 with the butanol productivity of 1.06 g/(L·h). The results provided the theoretical basis for the further optimization of butanol continuous fermentation under micro-aerobic conditions.

Key words: butanol, micro-aerobic fermentation, continuous fermentation, dilution rate, productivity

中图分类号:

Q815

李冬月, 李祥龙, 林樟楠, 刘宏娟, 张建安. 共生菌系TSH06连续发酵生产丁醇[J]. 化工进展, 2017, 36(04): 1418-1423.

LI Dongyue, LI Xianglong, LIN Zhangnan, LIU Hongjuan, ZHANG Jian'an. The continuous butanol fermentation of symbiotic system TSH06[J]. Chemical Industry and Engineering Progress, 2017, 36(04): 1418-1423.

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共生菌系TSH06连续发酵生产丁醇

The continuous butanol fermentation of symbiotic system TSH06

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