Zn2+、Ca2+和Mn2+对丙酮丁醇发酵的协同影响

doi:10.16085/j.issn.1000-6613.2015.10.031

化工进展 ›› 2015, Vol. 34 ›› Issue (10): 3719-3724.DOI: 10.16085/j.issn.1000-6613.2015.10.031

Zn²⁺、Ca²⁺和Mn²⁺对丙酮丁醇发酵的协同影响

付友思, 吴又多, 陈丽杰

大连理工大学生命科学与技术学院, 辽宁大连 116024

收稿日期:2015-01-27 修回日期:2015-03-24 出版日期:2015-10-05 发布日期:2015-10-05
通讯作者: 陈丽杰,硕士,副教授,主要从事生物能源方面研究。E-mailljchen@dlut.edu.cn。
作者简介:付友思(1990—),男,硕士研究生。
基金资助:
国家自然科学基金(21376044)及国家高技术研究发展计划(2011AA02A208、2012AA021205)项目。

Synergic effect of Zn²⁺,Ca²⁺,Mn²⁺ on acetone-butanol-ethanol fermentation

FU Yousi, WU Youduo, CHEN Lijie

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China

Received:2015-01-27 Revised:2015-03-24 Online:2015-10-05 Published:2015-10-05

摘要/Abstract

摘要： 在丙酮丁醇发酵中共同添加0.001g/L ZnSO₄·7H₂O和4.0g/L CaCO₃时,丁醇及总溶剂产量达到14.41g/L和23.69g/L,发酵终点乙酸和丁酸浓度为2.33g/L和1.02g/L。当共同添加0.001g/L ZnSO₄·7H₂O、4.0g/L CaCO₃和0.8g/LMnSO₄·H₂O时,丁醇的比生成速率为0.48g/(g·h),相对于共同添加Zn²⁺和Ca²⁺条件下的丁醇比生成速率0.23g/(g·h)提高了108.69%,而发酵终点乙酸和丁酸浓度分别为1.99g/L和0.54g/L,同比分别降低了14.59%和47.06%。Zn²⁺、Ca²⁺和Mn²⁺ 3种金属离子对丙酮丁醇发酵具有正向协同调控作用。

关键词: 丁醇发酵, 丙酮丁醇梭菌, 金属元素, 协同效应

Abstract: In acetone-butanol-ethanol (ABE) fermentation, when both 0.001g/L ZnSO₄·7H₂O and 4.0g/L CaCO₃ were supplemented to the fermentation medium, the butanol and ABE production increased to 14.41g/L and 23.69g/L, respectively, while acetate and butyrate production in turn raised to 2.33g/L and 1.02g/L at the end of the fermentation. When 0.001g/L ZnSO₄·7H₂O, 4.0g/L CaCO₃ and 0.8g/L MnSO₄·H₂O were all supplemented to the fermentation medium, the specific butanol production rate increased from 0.23g/(g·h) to 0.48g/(g·h), which increased by 108.69% compared to the control under both Zn²⁺ and Ca²⁺ supplementation condition. Moreover the acetate and butyrate production decreased to 1.99g/L and 0.54g/L, which decreased by 14.59% and 47.06% compared to the control under both Zn²⁺ and Ca²⁺ supplementation condition. These three metal elements of Zn²⁺, Ca²⁺ and Mn²⁺ show dramatic synergic effects on ABE fermentation.

Key words: butanol fermentation, Clostridium acetobutylicum, metal elements, synergic effect

中图分类号:

TQ923

付友思, 吴又多, 陈丽杰. Zn²⁺、Ca²⁺和Mn²⁺对丙酮丁醇发酵的协同影响[J]. 化工进展, 2015, 34(10): 3719-3724.

FU Yousi, WU Youduo, CHEN Lijie. Synergic effect of Zn²⁺,Ca²⁺,Mn²⁺ on acetone-butanol-ethanol fermentation[J]. Chemical Industry and Engineering Progree, 2015, 34(10): 3719-3724.

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Zn²⁺、Ca²⁺和Mn²⁺对丙酮丁醇发酵的协同影响

Synergic effect of Zn²⁺,Ca²⁺,Mn²⁺ on acetone-butanol-ethanol fermentation

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