外添少量电子受体强化丙丁梭菌丙酮-丁醇-乙醇发酵的丁醇合成

doi:10.16085/j.issn.1000-6613.2017-1296

化工进展 ›› 2018, Vol. 37 ›› Issue (05): 1940-1948.DOI: 10.16085/j.issn.1000-6613.2017-1296

外添少量电子受体强化丙丁梭菌丙酮-丁醇-乙醇发酵的丁醇合成

谢方¹, 王浩¹, 许萌¹, 丁健¹, 罗洪镇², 史仲平¹

1 江南大学生物工程学院, 工业生物技术教育部重点实验室, 江苏无锡 214122;
2 淮阴工学院生命科学与食品工程学院, 江苏淮安 223003

收稿日期:2017-06-26 修回日期:2017-10-02 出版日期:2018-05-05 发布日期:2018-05-05
通讯作者: 史仲平,教授,博士生导师,研究方向为发酵过程优化与控制。
作者简介:谢方(1993-),女,硕士研究生。
基金资助:
江苏省自然科学基金项目（BK20170459）。

Enhancing butanol synthesis in ABE fermentation by Clostridium acetobutylicum via supplementing small amount of electron receptors

XIE Fang¹, WANG Hao¹, XU Meng¹, DING Jian¹, LUO Hongzhen², SHI Zhongping¹

1 The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China;
2 School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, China

Received:2017-06-26 Revised:2017-10-02 Online:2018-05-05 Published:2018-05-05

摘要/Abstract

摘要： 强化利用丙丁梭菌发酵生产丁醇的主要方法有：添加电子载体强化NADH再生速率、通CO气体抑制氢化酶活性、外添少量丁酸等。但是，上述方法存在着总溶剂产量低、精制成本高、辅料价格昂贵等缺点。本研究通过向丙酮-丁醇-乙醇（ABE）发酵液添加少量电子受体（Na₂SO₄/CaSO₄，2g/L），使得梭菌胞内的电子穿梭传递系统的电子流和质子流发生改变，较多电子e^-和质子H⁺走向NADH合成途径，有利于丁醇合成；电子受体添加还可以促进对梭菌生存/丁醇合成的“有益”氨基酸、特别是缬氨酸的胞内积累/分泌，进一步强化了丁醇生产。在7L罐规模的发酵条件下、添加2g/L的电子受体Na₂SO₄，ABE发酵的丁醇浓度达到12.96g/L的最高水平，丁醇/丙酮比也有提高，分别比对照组提高35%和10%。添加Na₂SO₄等廉价电子受体提高了ABE发酵中的丁醇浓度，虽然提高幅度有限，但却可为利用发酵工程技术提高丁醇浓度和丁醇/丙酮比提供一种新的途径。

关键词: 丙酮-丁醇-乙醇发酵, 丙酮丁醇梭菌, 电子受体, 电子/质子迁移, 丁醇

Abstract: The common techniques/methods for enhancing butanol synthesis in ABE fermentation by Clostridium acetobutylicum include adding electron carrier to increase NADH regeneration rate,repressing hydrogenase via aerating CO,supplementing small amount of butyrate,etc. However,those methods suffer from the problems of total solvent decrease,purification cost-up to remove pigments,high butyrate cost,etc. In this study,small amount of electron receptors (Na₂SO₄/CaSO₄,2g/L)were added into the ABE fermentation broth:leading to the electron/proton flow transition/changes in the intracellular electron shuttle-transportation system,directing more electron/proton into the NADH regeneration route which enhanced for butanol synthesis; promoting the intracellular accumulation or secretion of those amino acids beneficial for cells survival/butanol synthesis,particularly valine. In the ABE fermentation in a 7L fermentor,by adding 2g/L electron receptor (Na₂SO₄),the butanol concentration reached the highest levels of 12.96g/L and butanol/acetone ratio also enhanced,the increments were 35% and 10%,respectively compared with those of control. The addition of the cheap electron receptor,such as Na₂SO₄,could increase the butanol concentration though the increment was limited,but it would supply a new and alternative fermentative method for enhancing butanol synthesis and butanol/acetone ratio.

Key words: ABE fermentation, Clostridium acetobutylicumelectron, receptor, electron/proton transition, butanol

中图分类号:

Q815

谢方, 王浩, 许萌, 丁健, 罗洪镇, 史仲平. 外添少量电子受体强化丙丁梭菌丙酮-丁醇-乙醇发酵的丁醇合成[J]. 化工进展, 2018, 37(05): 1940-1948.

XIE Fang, WANG Hao, XU Meng, DING Jian, LUO Hongzhen, SHI Zhongping. Enhancing butanol synthesis in ABE fermentation by Clostridium acetobutylicum via supplementing small amount of electron receptors[J]. Chemical Industry and Engineering Progress, 2018, 37(05): 1940-1948.

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外添少量电子受体强化丙丁梭菌丙酮-丁醇-乙醇发酵的丁醇合成

Enhancing butanol synthesis in ABE fermentation by Clostridium acetobutylicum via supplementing small amount of electron receptors

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