关键词: 代谢通量分析, 物料平衡, 同位素示踪, 基因组规模代谢网络, 酶

Abstract: Metabolic flux analysis can effectively predict the metabolic status of microorganism and guide the process of biosynthesis. It has been successfully applied in many fields. This paper summarizes the applications of three techniques of metabolic flux analysis in the field of industrial biotechnology，which were respectively based on material balance，isotope tracer techniques and genome-scale network. The advantages and limitation of these technologies are presented. Further the application of the two types of flux analysis techniques based on material balance and isotope tracer in the field of enzyme synthesis is discussed. The possible problem of the application of metabolic flux analysis in complex metabolites system is pointed out. At last，the paper indicates that how to bring the information，such as signal transduction，transcription regulation into metabolic network will become the future focus of metabolic analysis in enzyme synthesis.

Key words: metabolic flux analysis, material balance, isotope tracer, genome-scale network, enzyme