关键词: 玫瑰孢链霉菌, 达托霉素, 响应面法, 优化,  ,

Abstract:   The fermentation medium of daptomycin，produced by Streptomyces roseosporus，was optimized by response surface methodology. It was found that initial pH，glucose，L-Asp and K₂SO₄ affected the daptomycin production significantly in Plackett–Burman experiment，but the affection of initial pH was predominant （p<0.01）. The initial pH was optimized by one-factor-at-a-time experiment and the optimal pH obtained was 8.6. Then through the steepest ascent method and central composite design（CCD），the optimal fementation medium obtained were：13.0 g/L glucose，2.6 g/L L-Asp and 4.1 g/L K₂SO₄，and the daptomycin production reached 373.98 mg/L，which was in agreement with predicted value（365.76 mg/L）and 2.25 folds higher than that under the initial culture conditions.

Key words: streptomyces roseosporus, daptomycin, response surface methodology, optimization,  ,