高效石油烃降解菌群的构建及其含油污泥修复性能

doi:10.16085/j.issn.1000-6613.2023-1760

化工进展 ›› 2024, Vol. 43 ›› Issue (11): 6468-6474.DOI: 10.16085/j.issn.1000-6613.2023-1760

• 资源与环境化工 • 上一篇

高效石油烃降解菌群的构建及其含油污泥修复性能

孙妩娟¹^,²(), 李倩¹, 李小玲³, 石华强³, 杨志成⁴, 柯从玉¹^,², 王嗣昌¹, 张群正¹

^1.西安石油大学化学化工学院，陕西西安 710065
^2.西安石油大学油气田化学陕西省高校工程研究中心，陕西西安 710065
^3.中国石油长庆油田分公司油气工艺研究院，陕西西安 710018
^4.宁夏公路勘察设计院有限责任公司，宁夏银川 750004

收稿日期:2023-10-09 修回日期:2024-05-24 出版日期:2024-11-15 发布日期:2024-12-07
通讯作者: 孙妩娟
作者简介:孙妩娟（1979—），博士，副教授，研究方向为油气田环境污染控制。E-mail：sunwj@xsyu.edu.cn。
基金资助:
陕西省重点研发计划(2022ZDLSF07-04);西安市科技计划(2022JH-RYFW-0114)

Construction of an efficient microbial community for petroleum hydrocarbon degradation and its remediation performance for oily soil

SUN Wujuan¹^,²(), LI Qian¹, LI Xiaoling³, SHI Huaqiang³, YANG Zhicheng⁴, KE Congyu¹^,², WANG Sichang¹, ZHANG Qunzheng¹

^1.College of Chemistry and Chemical Engineering, Xi’an Shiyou University, Xi’an 710065, Shaanxi, China
^2.Shaanxi University Engineering Research Center of Oil and Gas Field Chemistry, Xi’an Shiyou University, Xi’an 710065, Shaanxi, China
^3.Institute of Oil and Gas Technology, PetroChina Changqing Oilfield Co. , Xi’an 710018, Shaanxi, China
^4.Ningxia Highway Survey and Design Institute Co. , Ltd. , Yinchuan 750004, Ningxia, China

Received:2023-10-09 Revised:2024-05-24 Online:2024-11-15 Published:2024-12-07
Contact: SUN Wujuan

摘要/Abstract

摘要：

为提高微生物修复含油土壤效果，本研究从油田含油土壤中筛选出4株高效石油烃降解菌R、J、C及K，通过16S rDNA分别鉴定为铜绿假单胞菌、热带假丝酵母菌、红城红球菌和枯草芽孢杆菌。这几株菌均能代谢生物表面活性剂，并对原油具有良好的降解性能。通过拮抗实验、表面张力测定以及对石油烃不同组分的降解性能分析，并结合优势互补原则最终确定将菌株R、K、J和C按1∶1∶1∶1进行复配以构建高效复合降解菌群。复合菌群能够发挥不同菌株之间的协同作用，进一步提高其对石油烃的降解性能，在相同条件下对石油烃降解率达到91.2%，明显优于单菌株。通过现场实验发现，采用复合菌剂对含油土壤进行生物强化处理60d后，土壤中的含油量从初始的4.97%下降到了0.34%，达到国家耕地二类标准，表明该菌群在含油土壤修复方面具有广阔的应用前景。

关键词: 含油土壤, 微生物修复, 生物降解, 菌种筛选, 复合菌群构建

Abstract:

In order to improve the effectiveness of microbial treatment of oily sludge, this study screened 4 efficient petroleum hydrocarbon degrading bacteria R, J, C and K from oilfield oily sludge, and identified them as Pseudomonas aeruginosa, Candida tropicalis, Rhodococcus erythropolis, and Bacillus subtilis through 16S rDNA. These strains of bacteria could metabolize biosurfactants and had good degradation performance on crude oil. Through antagonistic experiments, surface tension measurements, and analysis of the degradation performance of different components of petroleum hydrocarbons, combined with the principle of complementary advantages, it was ultimately determined to combine strains R, K, J, and C in a ratio of 1∶1∶1∶1 to construct an efficient composite degradation microbial community. The composite microbial community could exert synergistic effects among different strains, further improving the degradation performance of petroleum hydrocarbons. Under the same conditions, the degradation rate of petroleum hydrocarbons reached 91.2%, which was significantly better than that of a single strain. Through field application, it was found that after 60 days of composite microbial community bioaugmentation treatment, the oil content in the soil decreased from the initial 4.97% to 0.34%, meeting the national second class standard for arable land. This indicated that the microbial community had broad application prospects in the remediation of oily soil.

Key words: oily soil, microbial remediation, biodegradation, bacterial screening, construction of composite microbial communities

中图分类号:

TE99

孙妩娟, 李倩, 李小玲, 石华强, 杨志成, 柯从玉, 王嗣昌, 张群正. 高效石油烃降解菌群的构建及其含油污泥修复性能[J]. 化工进展, 2024, 43(11): 6468-6474.

SUN Wujuan, LI Qian, LI Xiaoling, SHI Huaqiang, YANG Zhicheng, KE Congyu, WANG Sichang, ZHANG Qunzheng. Construction of an efficient microbial community for petroleum hydrocarbon degradation and its remediation performance for oily soil[J]. Chemical Industry and Engineering Progress, 2024, 43(11): 6468-6474.

图/表 9

图1 发酵液表面张力及乳化指数

图2 菌株对原油降解效果

表1 4株菌株的复配性

菌株	R	J	C	K
R	—	无抑菌圈	无抑菌圈	无抑菌圈
J	无抑菌圈	—	无抑菌圈	无抑菌圈
C	无抑菌圈	无抑菌圈	—	无抑菌圈
K	无抑菌圈	无抑菌圈	无抑菌圈	—

图3 不同菌株对原油四组分降解效率

图4 微生物对油烃中不同组分的降解率

图5 原油浓度随时间的变化

图6 1/v和1/S拟合曲线

图7 现场处理工艺流程

图8 含油土壤中原油降解率与菌浓随时间变化趋势

参考文献 26

1	NWANKWEGU Amechi S, ORJI Michael U, ONWOSI Chukwudi O. Studies on organic and in-organic biostimulants in bioremediation of diesel-contaminated arable soil[J]. Chemosphere, 2016, 162: 148-156.
2	VARJANI Sunita J. Microbial degradation of petroleum hydrocarbons[J]. Bioresource Technology, 2017, 223: 277-286.
3	郭鹏, 庄建全, 纪艳娟, 等. 油田含油污泥特点与处理技术[J]. 精细石油化工进展, 2016, 17(3): 27-31.
	GUO Peng, ZHUANG Jianquan, JI Yanjuan, et al. Discussion on characteristics of oily sludge and technologies for treating oily sludge of oilfield[J]. Advances in Fine Petrochemicals, 2016, 17(3): 27-31.
4	侯影飞, 黄朝琦, 秦志文, 等. 含油污泥处理技术研究进展[J]. 当代化工, 2020, 49(3): 631-637.
	HOU Yingfei, HUANG Zhaoqi, QIN Zhiwen, et al. Research progress of oily sludge treatment technology[J]. Contemporary Chemical Industry, 2020, 49(3): 631-637.
5	ALAIDAROOS Bothaina A. Advancing eco-sustainable bioremediation for hydrocarbon contaminants: challenges and solutions[J]. Processes, 2023, 11(10): 3036.
6	邹春景, 傅晓文, 马荣辉, 等. 石油污染土壤的生物-化学联合修复技术研究进展[J]. 现代化工, 2023, 43(10): 36-40.
	ZOU Chunjing, FU Xiaowen, MA Ronghui, et al. Research progress on biology-chemistry combining remediation technology for petroleum contaminated soil[J]. Modern Chemical Industry, 2023, 43(10): 36-40.
7	LIU Yue, ZHANG Junhui, YANG Shaoqi, et al. Biodegradation and removal of heavy oil using Pseudomonas sp. and Bacillus spp. isolated from oily sludge and wastewater in Xinjiang Oilfield, China[J]. Journal of Environmental Chemical Engineering, 2023, 11(3): 110123.
8	AGUELMOUS Anas, FELS Loubna EL, SOUABI Salah, et al. The fate of total petroleum hydrocarbons during oily sludge composting: A critical review[J]. Reviews in Environmental Science and Bio-Technology, 2019, 18(3): 473-493.
9	VARJANI Sunita, UPASANI Vivek N, PANDEY Ashok. Bioremediation of oily sludge polluted soil employing a novel strain of Pseudomonas aeruginosa and phytotoxicity of petroleum hydrocarbons for seed germination[J]. Science of the Total Environment, 2020, 737: 139766.
10	宜慧, 常波, 杨玲引, 等. 高效石油烃降解菌的筛选及其对原油污染土壤的修复[J]. 化工环保, 2018, 38(4): 461-465.
	YI Hui, CHANG Bo, YANG Lingyin, et al. Screening of high-efficiency petroleum hydrocarbon degrading strains and remediation of crude oil contaminated soil[J]. Environmental Protection of Chemical Industry, 2018, 38(4): 461-465.
11	KE Congyu, QIN Fangling, YANG Zhigang, et al. Bioremediation of oily sludge by solid complex bacterial agent with a combined two-step process[J]. Ecotoxicology and Environmental Safety, 2021, 208: 111673.
12	KE Congyu, CHEN Liyang, QIN Fangling, et al. Biotreatment of oil sludge containing hydrocarbons by Proteus mirabilis SB[J]. Environmental Technology & Innovation, 2021, 23: 101654.
13	MISHRA S, JYOT J, KUHAD R C, et al. In situ bioremediation potential of an oily sludge-degrading bacterial consortium[J]. Current Microbiology, 2001, 43(5): 328-335.
14	BILEN OZYUREK Sezen, SEYIS BILKAY Isil. Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum-contaminated waste mud pit[J]. SN Applied Sciences, 2020, 2(2): 272.
15	OWSIANIAK Mikołaj, Łukasz CHRZANOWSKI, SZULC Alicja, et al. Biodegradation of diesel/biodiesel blends by a consortium of hydrocarbon degraders: Effect of the type of blend and the addition of biosurfactants[J]. Bioresource Technology, 2009, 100(3): 1497-1500.
16	GANESAN Mirunalini, MANI Ravi, Sakthinarenderan SAI, et al. Bioremediation by oil degrading marine bacteria: An overview of supplements and pathways in key processes[J]. Chemosphere, 2022, 303: 134956.
17	SIDDIQUI Zarreena, GROHMANN Elisabeth, MALIK Abdul. Degradation of alkane hydrocarbons by Priestia megaterium ZS16 and sediments consortia with special reference to toxicity and oxidative stress induced by the sediments in the vicinity of an oil refinery[J]. Chemosphere, 2023, 317: 137886.
18	VEERASAMY Veeramani, JAGANNATHAN Uma Maheswari, ARAKKALA Sherry Davis, et al. Exploring the bacterial genetic diversity and community structure of crude oil contaminated soils using microbiomics[J]. Environmental Research, 2023, 236: 116779.
19	LIU Na, WANG Liping, CAO Danfu, et al. Remediation of petroleum contaminated soil by persulfate oxidation coupled with microbial degradation[J]. Journal of Environmental Chemical Engineering, 2023, 11(3): 109910.
20	ANDREOLLI Marco, VILLANOVA Valeria, ZANZONI Serena, et al. Characterization of trehalolipid biosurfactant produced by the novel marine strain Rhodococcus sp. SP1d and its potential for environmental applications[J]. Microbial Cell Factories, 2023, 22(1): 126.
21	孙妩娟, 王琴婷, 王历历, 等. 热带假丝酵母菌对含油污泥的修复潜力研究[J]. 石油与天然气化工, 2022, 51(4): 113-118.
	SUN Wujuan, WANG Qinting, WANG Lili, et al. Study on remediation potential of oily sludge by Candida tropicalis [J]. Chemical Engineering of Oil & Gas, 2022, 51(4): 113-118.
22	GENNEQUIN Cédric, SIFFERT Stéphane, COUSIN Renaud, et al. Co-Mg-Al hydrotalcite precursors for catalytic total oxidation of volatile organic compounds[J]. Topics in Catalysis, 2009, 52(5): 482-491.
23	KOHLMEIER Stefanie, SMITS Theo H M, FORD Roseanne M, et al. Taking the fungal highway: Mobilization of pollutant-degrading bacteria by fungi[J]. Environmental Science & Technology, 2005, 39(12): 4640-4646.
24	辛蕴甜, 赵晓祥. 石油降解菌的降解性能及降解动力学研究[J]. 安全与环境学报, 2013, 13(5): 21-25.
	XIN Yuntian, ZHAO Xiaoxiang. Degradation features and degradation kinetics of oil-degrading bacteria[J]. Journal of Safety and Environment, 2013, 13(5): 21-25.
25	白智勇, 李博, 杨琦. 两株菌对萘的降解特性对比[J]. 油气田地面工程, 2013, 32(3): 48-49.
	BAI Zhiyong, LI Bo, YANG Qi. Comparison of degradation characteristics of naphthalene by two strains of bacteria[J]. Oil-Gas Field Surface Engineering, 2013, 32(3): 48-49.
26	HAMIDI Yasser, AHMAD ATAEI Seyed, SARRAFI Amir. Biodegradation of total petroleum hydrocarbons in oily sludge: A comparative study of biostimulation, bioaugmentation, and combination of methods[J]. Journal of Chemical Technology \& Biotechnology, 2021, 96(5): 1302-1307.

[1]	黄鸿, 欧阳浩民, 杨依静, 李昌霖, 陈烁娜. 硫化零价铁-微生物复合吸附剂对磷酸三(2-氯乙基)酯的吸附-降解机制[J]. 化工进展, 2024, 43(8): 4704-4713.
[2]	刘君, 胥志祥, 朱春游, 岳中秋, 潘学军. 典型环境微塑料的微生物降解途径及分子机制[J]. 化工进展, 2024, 43(7): 4059-4071.
[3]	杨静, 李博, 李文军, 刘晓娜, 汤刘元, 刘月, 钱天伟. 焦化污染场地中萘降解菌的分离及降解特性[J]. 化工进展, 2023, 42(8): 4351-4361.
[4]	李光文, 华渠成, 黄作鑫, 达志坚. 聚甲基丙烯酸酯类黏度指数改进剂的研究进展[J]. 化工进展, 2023, 42(3): 1562-1571.
[5]	付佳, 谌伦建, 徐冰, 华绍烽, 李从强, 杨明坤, 邢宝林, 仪桂云. 模拟煤炭气化废水中苯酚的微生物降解[J]. 化工进展, 2023, 42(1): 526-537.
[6]	刘雅娟. 浸没式PAC-AMBRs系统中PAC缓解膜污染的研究进展[J]. 化工进展, 2023, 42(1): 457-468.
[7]	岳瑶, 蒲梦凡, 王文瑞, 赵俭波, 曹辉. 聚天冬氨酸凝胶的制备及生物降解性[J]. 化工进展, 2022, 41(8): 4491-4497.
[8]	吕莹, 胡学武, 陈素素, 刘兴宇, 陈勃伟, 张明江. 多环芳烃污染土壤的微生物修复技术研究进展[J]. 化工进展, 2022, 41(6): 3249-3262.
[9]	汪润民, 张晓东, 徐成华, 于丹丹, 余冉. 高效重质石油降解菌群构建及降解性能评价[J]. 化工进展, 2022, 41(10): 5653-5660.
[10]	陈露蕊, 曹利锋. 温度对厌氧耗氢产甲烷的影响研究进展[J]. 化工进展, 2021, 40(S1): 326-333.
[11]	潘云飞, 唐正, 彭欣怡, 高品. 石油烃污染土壤微生物修复技术研究现状及进展[J]. 化工进展, 2021, 40(8): 4562-4572.
[12]	包清华, 黄立信, 修建龙, 俞理, 崔庆锋, 马原栋, 伊丽娜. 油气田含油污泥生物处理技术研究进展[J]. 化工进展, 2021, 40(5): 2762-2773.
[13]	林晓琪, 陈维胜, 张芹芹. 聚氨酯在生物医学领域的研究进展[J]. 化工进展, 2020, 39(S1): 212-218.
[14]	刘群, 张玉苍. 改性淀粉基生物降解塑料的研究进展[J]. 化工进展, 2020, 39(8): 3124-3134.
[15]	崔堂武, 袁波, 凌晨, 方彬任, 毛向阳, 费强. 木质素降解酶的酶活测试方法的评价与分析[J]. 化工进展, 2020, 39(12): 5189-5202.

高效石油烃降解菌群的构建及其含油污泥修复性能

Construction of an efficient microbial community for petroleum hydrocarbon degradation and its remediation performance for oily soil

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 26

相关文章 15

编辑推荐

Metrics

本文评价