多巴胺表面修饰角蛋白/聚乳酸纳米纤维膜的制备及性能

doi:10.16085/j.issn.1000-6613.2021-0164

摘要/Abstract

摘要：

通过紫外引发多巴胺氧化自聚生成聚多巴胺，对角蛋白/聚乳酸纳米纤维膜进行表面修饰，以改善其薄膜亲水性差、力学性能不足和细胞活性较低等问题。通过扫描电子显微镜、傅里叶变换红外光谱、热重分析、接触角实验、电子万能试验机、细胞活性毒性实验和细胞黏附性实验对表面修饰前后纳米纤维膜的形貌、结构、热稳定性和热降解率、亲水性、力学性能、细胞活性毒性和黏附性进行测试和表征。结果表明，聚多巴胺成功地黏附在纤维的表面，表面修饰后的纤维平均直径增大，由(356±78)nm增大至(507±98)nm，接触角由103.34?降至82.46?，弹性模量与断裂伸长率分别增大了2.75~5.33MPa和31.75%~51.50%，接种24h后细胞活性由72%增大至221%，增大了149%。

关键词: 多巴胺表面修饰, 角蛋白, 静电纺丝, 伤口敷料

Abstract:

The keratin/polylactic acid nanofiber membrane was surface modified by ultraviolet-induced dopamine oxidation and self-polymerization to improve the problems of poor hydrophilicity, insufficient mechanical properties and low cell activity. Through scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis, contact angle experiment, electronic universal testing machine, cell activity toxicity experiment and cell adhesion experiment, the morphology, structure, and thermal stability of the nanofiber membrane before and after surface modification Properties and thermal degradation rate, hydrophilicity, mechanical properties, cell activity toxicity and adhesion were tested and characterized. The results showed that: polydopamine successfully adhered to the surface of the fiber, the average diameter of the modified fiber increased from (356±78)nm to (507±98)nm, the contact angle decreased from 103.34? to 82.46?, the modulus of elasticity and fracture the elongation increased by 2.75—5.33MPa and 31.75%—51.50% respectively, and the cell viability increased from 72% to 221% after 24h inoculation, an increase of 149%.

Key words: dopamine surface modification, keratin, electrospinning, wound dressing

中图分类号:

TQ342⁺.87

王喜倩, 尹国强, 郭清兵, 何明. 多巴胺表面修饰角蛋白/聚乳酸纳米纤维膜的制备及性能[J]. 化工进展, 2022, 41(1): 373-381.

WANG Xiqian, YIN Guoqiang, GUO Qingbing, HE Ming. Preparation and properties of FK/PLA nanofiber membrane modified with dopamine[J]. Chemical Industry and Engineering Progress, 2022, 41(1): 373-381.

图/表 9

图1 不同浓度DA溶液浸泡的FK/PLA纳米纤维膜的SEM图

图2 不同浓度DA溶液浸泡的FK/PLA纳米纤维膜的平均直径图

图3 纯组分及不同浓度DA溶液浸泡的FK/PLA纳米纤维膜的FTIR图

图4 不同浓度DA溶液浸泡的FK/PLA纳米纤维膜的TG及DTG图

表1 不同浓度DA溶液浸泡的FK/PLA纳米纤维膜的热性能分析结果

DA溶液浓度/g ? L^-1	T_d1/℃	T_max/℃	热降解率/%
0	302.81	392.67	95.98
0.5	329.89	394.19	96.48
1.0	332.21	395.71	97.60
2.0	341.64	397.42	97.16
3.0	338.93	396.47	97.13
4.0	332.85	394.20	96.43

图5 不同浓度DA溶液浸泡的FK/PLA纳米纤维膜的弹性模量和断裂伸长率

图6 不同浓度DA溶液浸泡的FK/PLA纳米纤维膜的接触角图

图7 不同浓度DA溶液浸泡的FK/PLA纳米纤维膜及其浸提液的细胞活力

图8 不同浓度DA溶液浸泡的FK/PLA纳米纤维膜的荧光图

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