静电纺丝法制备ZnO构建酪氨酸酶生物传感器检测邻苯二酚

doi:10.16085/j.issn.1000-6613.2019-1746

摘要/Abstract

摘要：

电化学生物传感器在环境监测、生物与食品分析等领域应用广泛。本文采用静电纺丝法制备了氧化锌微纳米纤维材料，并负载酪氨酸酶（Tyr）构建了检测邻苯二酚的生物传感电极。利用X射线粉末衍射（XRD）、扫描电子显微镜（SEM）表征了氧化锌微纳米纤维的形貌结构，采用循环伏安法（CV）和计时电流法（IT）优化了检测邻苯二酚的工作条件。结果表明，制备的Tyr/ZnO/CS/GCE生物传感电极具有良好的电化学性能，在反应过程中为扩散控制，对邻苯二酚的检测在5～50μmol/L的浓度范围内具有较好的线性关系，最低检测限为1.9041μmol/L，灵敏度为376.31μA/(mmol·L·cm²)，氧化锌的加入可增强酪氨酸酶的稳定性，在尿素、多巴胺和葡萄糖3种电化学活性相近物质存在的情况下仍对邻苯二酚的检测有较好的选择性，且具有良好的循环稳定性。

关键词: 静电纺丝法, 氧化锌, 酪氨酸酶, 邻苯二酚, 生物传感器

Abstract:

Electrochemical biosensors are widely used in environmental monitoring, biological analysis , food analysis and others . In this paper, zinc oxide micro/nanofibers were prepared by electrospinning, and a biosensor electrode for catechol detection was constructed by tyrosinase(Tyr). The morphology and structure of ZnO micro/nanofibers were characterized by XRD and SEM. The working conditions of pyrocatechol detection system were optimized by CV and IT. The results confirmed that the prepared Tyr/ZnO/CS/GCE biosensor electrode had good electrochemical performance. The reaction process was controlled by diffusion, and the detection of catechol had a good linear relationship in the concentration range of 5—50μmol/L. The minimum detection limit was 1.9041μmol/L, and the sensitivity was 376.31μA/(mmol·L·cm²). The addition of zinc oxide could enhance the stability of tyrosinase. In the presence of urea, dopamine and glucose, of which the electrochemical activities were closed to that of catechol, the detection of catechol was still selective. In addition, the electrode had good cycle stability.

Key words: electrospinning, zinc oxide, tyrosinase, catechol, biosensor

中图分类号:

X502

李纯, 孙丽霞, 孙建华, 周利琴, 徐大鹏, 张友全, 廖丹葵. 静电纺丝法制备ZnO构建酪氨酸酶生物传感器检测邻苯二酚[J]. 化工进展, 2020, 39(7): 2795-2801.

Chun LI, Lixia SUN, Jianhua SUN, Liqin ZHOU, Dapeng XU, Youquan ZHANG, Dankui LIAO. Fabrication of Tyr biosensor for detection of catechol based on the ZnO with electrospinning[J]. Chemical Industry and Engineering Progress, 2020, 39(7): 2795-2801.

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