半纤维素酶添加对碱处理甘蔗渣结构及酶解的影响

doi:10.16085/j.issn.1000-6613.2016.s1.044

化工进展 ›› 2016, Vol. 35 ›› Issue (S1): 270-275.DOI: 10.16085/j.issn.1000-6613.2016.s1.044

半纤维素酶添加对碱处理甘蔗渣结构及酶解的影响

高月淑¹, 许敬亮², 袁振宏², 张宁¹, 蒋剑春¹

1. 中国林业科学研究院林产化学工业研究所, 江苏省生物质能源与材料重点实验室, 江苏南京 210042;
2. 中国科学院广州能源研究所, 广东广州 510640

收稿日期:2015-12-18 修回日期:2016-04-08 出版日期:2016-06-30 发布日期:2016-07-08
通讯作者: 蒋剑春,博士生导师,主要从事林产化学工业加工和生物质能源开发技术研究。E-mail bio-energy@163.com。
作者简介:高月淑(1986-),女,博士,助理研究员,主要从事生物质化学品研究工作。
基金资助:
国家"十二五"科技支撑计划(2014BAD02B02)、广州市科技攻关项目(2013J4300026)及中国科学院可再生能源重点实验室开放基金项目(y507k71001)。

Effects of the adding of hemicellulase on chemical compositon of sugarcane bagasse and enzymatic hydrolysis

GAO Yueshu¹, XU Jingliang², YUAN Zhenhong², ZHANG Ning¹, JIANG Jianchun¹

1. Jiangsu Province Key Laboratory of Bionmass Energy and Mate rials, Institute of Chemical Industry of Forest Products, Chinese Academy of Forest, Nanjing 210042, Jiangsu, China;
2. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, Guangdong, China

Received:2015-12-18 Revised:2016-04-08 Online:2016-06-30 Published:2016-07-08

摘要/Abstract

摘要： 半纤维素作为木质纤维素的重要组分之一,通过氢键与纤维素的微纤丝结合,严重阻碍了纤维素表面与纤维素酶的接触,降低了酶解的效率。该试验以碱处理甘蔗渣作为底物,通过添加不同量的半纤维素酶去除不同比例的半纤维素。通过成分分析、X射线衍射(XRD)和扫描电镜(SEM)等手段分析添加半纤维素酶前后残渣的结构和酶解特性变化,发现随着半纤维素酶添加量的增大,残渣中木质素所占的比例逐渐增大,结晶指数逐渐增大,电镜表面沟壑逐渐加深,纤维束之间结构变得疏松。以半纤维素酶处理过的甘蔗渣作为底物,按照5FPU/g底物加入纤维素酶水解72h,与不添加半纤维素酶对照组相比,添加1600U/g半纤维素酶处理的试验组木聚糖的转化率提高了74.24%,葡聚糖转化率提高了35.30%。通过半纤维素酶添加可以有效促进纤维素酶解过程的进行,节约反应时间提高酶解转化率。

关键词: 生物质, 半纤维素酶, 酶解

Abstract: Hemicellulose, as one of the main compostions of lignocellulosic materials, leads to a complex structure characteristic, the presence of which hinders the access of enzyme to cellulose thus reduces efficiency of the hydrolysis.Different percentages of hemicellulose were removed from alkali-pretreated SCB since different concentrations of hemicellulase were added.Based on the results of compostitution analysis, SEM analysis, XRD analysis, as well as the enzymatic digestibility, it could be found that with the concentrations of hemicellulase added increasing, the content of lignin and crystallie index were larger and surface gap gradually deepened.Before the cellulase loading of 5FPU/g sustrate added, extra 1600U/g hemicellulase were used to treat the alkali-pretreated SCB which showed that the xylan and glucose conversion rate increasing 74.24% and 35.30%, respectively.It demonstrated that the removal of hemicellulose could effectively promote the enzymatic hydrolysis process, save the reaction time and increse the enzymatic conversion rate.

Key words: biomass, hemicellulase, enzymatic hydrolysis

中图分类号:

Q556

高月淑, 许敬亮, 袁振宏, 张宁, 蒋剑春. 半纤维素酶添加对碱处理甘蔗渣结构及酶解的影响[J]. 化工进展, 2016, 35(S1): 270-275.

GAO Yueshu, XU Jingliang, YUAN Zhenhong, ZHANG Ning, JIANG Jianchun. Effects of the adding of hemicellulase on chemical compositon of sugarcane bagasse and enzymatic hydrolysis[J]. Chemical Industry and Engineering Progree, 2016, 35(S1): 270-275.

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半纤维素酶添加对碱处理甘蔗渣结构及酶解的影响

Effects of the adding of hemicellulase on chemical compositon of sugarcane bagasse and enzymatic hydrolysis

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