化工进展 ›› 2015, Vol. 34 ›› Issue (02): 508-513.DOI: 10.16085/j.issn.1000-6613.2015.02.032

• 生物与医药化工 • 上一篇    下一篇

红豆杉单组分多糖的超滤预处理及连续分离工艺

张菲菲1,2, 田慧2, 梁亚非3, 吴绵斌1,2, 林建平1,2, 杨立荣1,2   

  1. 1. 浙江大学生物质化工教育部重点实验室, 浙江 杭州 310027;
    2. 浙江大学化学工程与生物工程学系, 浙江 杭州 310027;
    3. 金华市人民医院, 浙江 金华 318100
  • 收稿日期:2014-07-03 修回日期:2014-08-08 出版日期:2015-02-05 发布日期:2015-02-05
  • 通讯作者: 吴绵斌,副教授,E-mail:wumb@zju.edu.cn;林建平,教授,E-mail:linjp@zju.edu.cn
  • 作者简介:张菲菲(1989-),女,硕士研究生,E-mail:857307211@qq.com;田慧(1983-),女,硕士研究生,E-mail:1031539118@qq.com田慧和张菲菲对本篇论文具有相同贡献
  • 基金资助:
    国家自然科学基金(21376215)、浙江省科技计划(2010C33122)、国家973计划(2011CB710800)及国家863计划(2012AA022302)项目

Ultrafiltration preprocessing and continuous separation process of single-component polysaccharides from Taxus Mairei

ZHANG Feifei1,2, TIAN Hui2, LIANG Yafei3, WU Mianbin1,2, LIN Jianping1,2, YANG Lirong1,2   

  1. 1. Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310027, Zhejiang, China;
    2. Department of Chemical and Biolaogical Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China;
    3. Jinhua People's Hospital, Jinhua 321000, Zhejiang, China
  • Received:2014-07-03 Revised:2014-08-08 Online:2015-02-05 Published:2015-02-05

摘要: 以红豆杉枝叶部位总多糖为原料,采用超滤方法对原料进行预处理,并对工艺进行优化,最佳优化条件为:截留相对分子质量为5kDa,温度为25℃,压力为20psi(1psi=6894.76kPa),pH值为6.8。 在此条件下,将浓度为10g/L的1L红豆杉多糖溶液超滤至125mL,再添加去离子水至1L,反复超滤4次,得到的红豆杉多糖的截留率和脱色率分别为87.2%和75.6%。进一步以红豆杉单组分多糖间歇色谱分离工艺参数为基础,采用自行设计制作的连续色谱设备,建立了连续离子交换色谱分离红豆杉酸性单组分多糖PST-1的分离工艺,处理量达到每批次100.08L,PST-1纯度在99.0%以上,收率达到50.0%。

关键词: 红豆杉, 多糖, 超滤, 连续色谱分离

Abstract: The total polysaccharides from Taxus Mairei were pretreated by ultrafiltration to remove pigments and the operating condition was optimized as follows:molecular cut-off 5 kDa,temperature 25℃,operating pressure 20psi and pH6.8. Under the aforementioned condition,the 1L solution containing 10g/L total polysaccharides was ultrafiltrated to 125mL first,then deionized water was added into condensed solution to 1L total volume and was repeated 4 times,respectively. The results showed that the retention rate and decolourization ratio of polysaccharides of Taxus Mairei were 87.2% and 75.6%,respectively. Furthermore,the pretreated total polysaccharides of Taxus Mairei were isolated by cation-exchange chromatography using a self-designed continuous chromatography equipment,the processing capacity of which reached 100.08L/d. Based on the operating parameters received from batch chromatography process,99.0% purity single-component polysaccharide (PST-1) was obtained and the average yield of PST-1 reached 50.0%.

Key words: Taxus Mairei, polysaccharides, ultrafiltration, continuous chromatography separation

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