关键词: 槐糖脂, 分离, 沉降, 过滤, 吸附, 超滤

Abstract:

A new process for the pretreatment of fermentation broth and the separation of sophorolipids in the industrial production process of sophorolipids (SLs) was optimized. The natural sedimentation method was used instead of the traditional ethyl acetate extraction method to obtain the lactonic sophorolipid from the lower fermentation broth. For the upper fermentation broth, the industrial common-used frame filtering was adopted to replace the traditional laboratory centrifugation method to remove biomass. Methods of resin adsorption and ultrafiltration were selected instead of the organic solvent extraction method and alcohol precipitation method to depigmentation, desalting, and deproteinization. Following this procedure, acidic sophorolipid with high purity was obtained from the upper fermentation broth. The sophorolipids content and composition were determined by HPLC, and it was found that the obtained sophorolipid products met the market standards and performed better than commercial sophorolipids. Hence, this pretreatment and separation process can not only enrich the varieties of sophorolipid products,but also can fill in the defects that the commercial products are a complex mixture of lactonic and acidic sophorolipids. Furthermore, the significantly reduced use of organic reagents during this process makes great improvements in the safety and feasibility of sophorolipid production.

Key words: sophorolipid, separation, sedimentation, filtration, adsorption, ultrafiltration