Chemical Industry and Engineering Progress ›› 2021, Vol. 40 ›› Issue (8): 4371-4380.DOI: 10.16085/j.issn.1000-6613.2020-1901

• Materials science and technology • Previous Articles     Next Articles

Synthesis and utilization of Pt and Pd nanoparticle-decorated MoS2 nanocomposites for fabrication of electrochemical biosensor

ZHOU Liya1(), ZHOU Xitong1, ZHAO Congli2, JIANG Yanjun1, MA Li1(), He Ying1   

  1. 1.School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China
    2.School of Health Science and Engineering, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
  • Received:2020-09-17 Online:2021-08-12 Published:2021-08-05
  • Contact: MA Li

基于铂、钯负载的MoS2纳米片构建生物传感器及应用

周丽亚1(), 周栖桐1, 赵聪俐2, 姜艳军1, 马丽1(), 贺莹1   

  1. 1.河北工业大学化工学院,天津 300130
    2.天津中医药大学健康科学与工程学院,天津 301617
  • 通讯作者: 马丽
  • 作者简介:周丽亚(1976—),女,博士,教授,研究方向为生物传感器和酶分子定向改造。E-mail:liyazhou@hebut.edu.cn
  • 基金资助:
    国家自然科学基金(21576068);河北省自然科学基金(B2017202056)

Abstract:

Pd and Pt nanoparticle-decorated MoS2 nanocomposites (Pt-Pd/MoS2) were synthesized and applied to prepare acetylcholinesterase (AChE) biosensors for organophosphorus pesticide detection. The nanoparticles were characterized by SEM, TEM and XPS. The AChE biosensor showed high affinity to acetylthiocholine chloride (ATCl) with Km of 883μmol/L. The prepared biosensors exhibited high sensitivity towards malathion and parathion methyl, with a linear range of 1×10-14mol/L to 1× 10-5mol/L and a detection limit of 4.69×10-14mol/L (S/N=3) and a linear range of 1×10-15mol/L to 1×10-5mol/L and a detection limit of 5.23×10-15mol/L (S/N=3), respectively. Moreover, the AChE/Pt-Pd/MoS2/GCE was used to detect OPs in real samples with the recovery of 91.4%—103%. Due to the synergistic effect of the highly dispersed bimetallic nanoparticles and the MoS2 nanosheets, the Pt-Pd/MoS2 nanocomposites provide a potential electrode material for biosensor fabrication.

Key words: noble metal, organophosphate pesticide, molybdenum disulfide (MoS2), acetylcholinesterase, biosensor

摘要:

制备了Pt和Pd纳米颗粒修饰的单层MoS2纳米片(Pt-Pd/MoS2),通过扫描电镜(SEM)、透射电镜(TEM)和X射线光电子能谱(XPS)对Pt-Pd/MoS2外部形貌、内部结构和组成进行表征分析,并基于Pt-Pd/MoS2修饰玻碳电极,并于表面固定乙酰胆碱酯酶(AChE),制备AChE生物传感器。对比了Pt和Pd双金属纳米颗粒、商品化Pt/C及Pt-Pd/MoS2的电化学性能,结果发现,Pt-Pd/MoS2的电化学性能明显优于其他两种。测定电极表面酶催化反应的动力学参数Km为883μmol/L;以马拉硫磷和甲基对硫磷为代表,考察制备电极的检测性能以及制备电极对有机磷农药的检测性能,马拉硫磷的检测范围是1×10-14~1×10-5mol/L,检测限为4.69×10-14mol/L;甲基对硫磷的检测范围是1×10-15~1×10-5mol/L,检测限为5.23×10-15mol/L(S/N=3);并应用于真实样品检测OPs的回收率为91.4%~103%,显示出良好的回收率和准确性,可应用于实际样品的分析。Pt-Pd/MoS2为二维纳米材料构建高效生物传感器提供了新思路。

关键词: 贵金属, 有机磷农药, 二硫化钼, 乙酰胆碱酯酶, 生物传感器

CLC Number: 

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