Biosynthesis of zinc oxide nanoparticles and its application to antibacterial

doi:10.16085/j.issn.1000-6613.2022-1026

Abstract

Abstract:

With the aggravation of environmental pollution and the widespread use of antibiotics, various diseases threatening human health have gradually broken out, and the problem of antibiotic resistance of pathogenic bacteria has become increasingly serious. This has prompted many researches to explore new antibacterial agents that are environmentally friendly, have strong antibacterial activity, and do not easily produce drug resistance. Nanotechnology has been proved to be an effective means to fight against pathogens. Zinc oxide nanoparticles have excellent antibacterial properties and are expected to be widely used as new metal ion antibacterial materials. Compared with traditional physical and chemical methods, the biological method of zinc oxide nanoparticles has the advantages of simple operation, high safety, and less environmental pollution. It has become a new trend in the development of nano synthesis technology. In this paper, the biosynthesis methods and synthesis mechanism of zinc oxide nanoparticles using plant, algae and microorganism extracts are first summarised. Then, the antibacterial mechanism and antibacterial applications of zinc oxide nanoparticles in the pharmaceutical industry, textile industry, food industry, agriculture and other related fields are discussed. Finally, the related research and application prospects of innovative multi-metal composite nanoparticles containing zinc oxide are further discussed, providing new ideas for the development of zinc oxide nanotechnology.

Key words: zinc oxide nanoparticles, biosynthesis, antibacterial mechanism, antibacterial application, polymetallic nanomaterials

摘要：

随着环境污染加剧与抗生素的广泛使用，各种威胁人类健康的疾病逐渐爆发，病原菌对抗生素的耐药性问题也愈发严重。这促使许多研究都集中在对绿色环保、抗菌活性强、不易产生耐药性的新型抗菌剂的探索上，并且纳米技术已被证明可作为对抗病原菌的有效手段。氧化锌纳米颗粒材料具有优异的抗菌抑菌性能，有望作为新型金属离子抗菌材料而被广泛应用。与传统物理化学方法相比，氧化锌纳米颗粒的生物方法具有操作简单、安全性高、对环境污染小等优势，已成为纳米合成技术发展的新趋势。本文首先综述了利用植物、藻类、微生物等提取物进行氧化锌纳米颗粒的生物合成方法与合成机理，总结了氧化锌纳米颗粒的抗菌机制，讨论了氧化锌纳米材料在医药行业、纺织工业、食品行业、农业等相关领域的抗菌应用，最后进一步展望了含有氧化锌的创新型多金属复合型纳米颗粒的相关研究与应用前景，为氧化锌纳米技术发展提供了新思路。

关键词: 氧化锌纳米颗粒, 生物合成, 抗菌机制, 抑菌应用, 多金属纳米材料

CLC Number:

TQ132.4⁺1

SI Yinfang, HU Yujie, ZHANG Fan, DONG Hao, SHE Yuehui. Biosynthesis of zinc oxide nanoparticles and its application to antibacterial[J]. Chemical Industry and Engineering Progress, 2023, 42(4): 2013-2023.

司银芳, 胡语婕, 张凡, 董浩, 佘跃惠. 生物合成氧化锌纳米颗粒材料及其抗菌应用[J]. 化工进展, 2023, 42(4): 2013-2023.

Figures/Tables 6

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原料	抑制菌种	粒径/nm	应用	参考文献	年份
植物
澳橙皮提取液、Zn(NO₃)₂溶液、羧甲基纤维素钠（CMC）	灰霉菌（Botrytis cinerea）、大肠杆菌（Escherichia coli）、金黄色葡萄球菌（Staphylococcus aureus）	33.1±11.7	在食品表面形成涂膜，抑制食品表面微生物的生长	[8]	2020
辣木叶提取物、硝酸锌氢氧化物	革兰氏阳性菌、革兰氏阴性菌	50	阻碍革兰氏阳性和革兰氏阴性细菌菌株的微生物生物膜形成	[9]	2021
百里香提取物、二水乙酸锌溶液	枯草芽孢杆菌（Bacillus subtilis subsp）	10~35	体外抗菌	[10]	2021
芦荟提取物、Zn(NO₃)₂溶液	大肠杆菌	452		[11]	2020
茉莉花提取物、葡萄糖酸锌水合物	大肠杆菌、屎肠球菌（Enterococcus Faecium）	2~4	抗菌剂	[12]	2016
接骨木（Sambucus ebulus）提取物、二水乙酸锌	蜡样芽孢杆菌（Bacillus cereus）、金黄色葡萄球菌、大肠杆菌	17	光催化降解亚甲基蓝染料污染物	[13]	2020
微生物
铜绿假单胞菌（P. aeruginosa）JS29、Zn(NO₃)₂溶液	金黄色葡萄球菌	40~50	药物替代品、抗菌	[14]	2021
高耐硫酸锌的植物乳杆菌LP4、Zn(NO₃)₂溶液		9~35	脂肪酶的固定化	[15]	2020
嗜水气单胞菌（Aeromonashydrophila）、ZnO	铜绿假单胞菌、黄曲霉	57.72	抗菌剂	[16]	2012
地衣芽孢杆菌（Bacilluslicheniformis）、二水乙酸锌		620	光催化降解有机污染物	[17]	2014
烟曲霉TFR-8、Zn(NO₃)₂溶液		1.2~6.8	生物纳米肥料，促进莱豆生长	[18]	2013
藻类
鞭毛藻（Dinoflagellate）、乙酸锌溶液	金黄色葡萄球菌、大肠杆菌	20~40	抗菌	[19]	2021
马尾藻（Scagassum）、乙酸锌二水合物溶液	人肝癌细胞系（HepG2）		抗血管生成和抗凋亡作用，可作为癌症治疗的补充药物	[20]	2018
江蓠（Gracilaria）	前列腺癌细胞	335	癌症治疗的补充药物	[21]	2014
马尾藻、脱水乙酸锌溶液		30~57	生物医学和药学	[22]	2014

原料	抑制菌种	粒径/nm	应用	参考文献	年份
植物
澳橙皮提取液、Zn(NO₃)₂溶液、羧甲基纤维素钠（CMC）	灰霉菌（Botrytis cinerea）、大肠杆菌（Escherichia coli）、金黄色葡萄球菌（Staphylococcus aureus）	33.1±11.7	在食品表面形成涂膜，抑制食品表面微生物的生长	[8]	2020
辣木叶提取物、硝酸锌氢氧化物	革兰氏阳性菌、革兰氏阴性菌	50	阻碍革兰氏阳性和革兰氏阴性细菌菌株的微生物生物膜形成	[9]	2021
百里香提取物、二水乙酸锌溶液	枯草芽孢杆菌（Bacillus subtilis subsp）	10~35	体外抗菌	[10]	2021
芦荟提取物、Zn(NO₃)₂溶液	大肠杆菌	452		[11]	2020
茉莉花提取物、葡萄糖酸锌水合物	大肠杆菌、屎肠球菌（Enterococcus Faecium）	2~4	抗菌剂	[12]	2016
接骨木（Sambucus ebulus）提取物、二水乙酸锌	蜡样芽孢杆菌（Bacillus cereus）、金黄色葡萄球菌、大肠杆菌	17	光催化降解亚甲基蓝染料污染物	[13]	2020
微生物
铜绿假单胞菌（P. aeruginosa）JS29、Zn(NO₃)₂溶液	金黄色葡萄球菌	40~50	药物替代品、抗菌	[14]	2021
高耐硫酸锌的植物乳杆菌LP4、Zn(NO₃)₂溶液		9~35	脂肪酶的固定化	[15]	2020
嗜水气单胞菌（Aeromonashydrophila）、ZnO	铜绿假单胞菌、黄曲霉	57.72	抗菌剂	[16]	2012
地衣芽孢杆菌（Bacilluslicheniformis）、二水乙酸锌		620	光催化降解有机污染物	[17]	2014
烟曲霉TFR-8、Zn(NO₃)₂溶液		1.2~6.8	生物纳米肥料，促进莱豆生长	[18]	2013
藻类
鞭毛藻（Dinoflagellate）、乙酸锌溶液	金黄色葡萄球菌、大肠杆菌	20~40	抗菌	[19]	2021
马尾藻（Scagassum）、乙酸锌二水合物溶液	人肝癌细胞系（HepG2）		抗血管生成和抗凋亡作用，可作为癌症治疗的补充药物	[20]	2018
江蓠（Gracilaria）	前列腺癌细胞	335	癌症治疗的补充药物	[21]	2014
马尾藻、脱水乙酸锌溶液		30~57	生物医学和药学	[22]	2014

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