Magnetite-loaded biochar for enhanced anaerobic microbial treatment of 2,4-dichlorophenol wastewater

doi:10.16085/j.issn.1000-6613.2021-2381

Abstract

Abstract:

Chlorophenol compounds are a kind of persistent organic pollutants, which are toxic to microorganisms during anaerobic wastewater treatment. In this experiment, the magnetite- modified biochar was added to improve the removal of chlorophenol in the upflow anaerobic sludge bed (UASB) reactors by enhancing the sludge conductivity and accelerating the iron reduction process. Results show that the removal rate of COD and 2,4-dichlorophenol in the reactor with magnetite-loaded biochar increases by 20% and 54.1%, respectively. Magnetite-loaded biochar has multiple functional groups and strong ability to accept/donate electrons, and thus the charge and discharge performances of sludge in the reactor with biochar are increased by 23.8%. Microbial community structure analysis shows that the addition of magnetite-loaded biochar increases the abundances of hydrolytic bacteria such as Mesotoga and bacteria capable of electron transfer function such as Petrimona, which accelerates the degradation of phenolic compounds.

Key words: biochar, magnetite, 2,4-dichlorophenol, extracellular electron transfer, iron reduction

摘要：

氯酚类化合物是一类对环境具有持续污染性的有毒有机物，在厌氧处理时具有生物毒害性。本文通过构建磁铁矿改性生物炭改善上流式厌氧污泥床（UASB）内的导电性与加快铁还原过程，强化厌氧微生物对氯酚废水的去除效果。结果表明，加入磁铁矿负载生物炭的反应器化学需氧量（COD）、2,4-二氯苯酚去除率分别提高了20%与54.1%。磁铁矿负载生物炭具有多官能团与强接受/贡献电子能力，反应器内污泥充放电性提升23.8%。微生物群落结构分析显示，磁铁矿负载生物炭的加入提高了Mesotoga等水解菌丰度，提高了Petrimonas等具有电子传递功能的细菌丰度，抵抗高有机负荷冲击，加速酚类化合物的降解与转化。

关键词: 生物炭, 磁铁矿, 2,4-二氯苯酚, 胞外电子传递, 铁还原

CLC Number:

YANG Liu, WANG Mingwei, ZHANG Yaobin. Magnetite-loaded biochar for enhanced anaerobic microbial treatment of 2,4-dichlorophenol wastewater[J]. Chemical Industry and Engineering Progress, 2022, 41(9): 5065-5073.

杨柳, 王名威, 张耀斌. 磁铁矿负载生物炭强化厌氧微生物处理2,4-二氯苯酚废水[J]. 化工进展, 2022, 41(9): 5065-5073.

Figures/Tables 11

References 31

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菌属	相对丰度（50mg·L^-1 2，4-DCP）/%				相对丰度（100mg·L^-1 2，4-DCP）/%
菌属	R1	R2	R3	R4	R1	R2	R3	R4
Methanothrix	43.24	25.74	33.63	34.64	32.74	20.06	38.17	36.32
Methanobacterium	34.72	61.63	50.26	50.49	45.46	67.02	41.20	49.29
Methanocorpusculum	0.62	5.83	5.34	4.91	4.23	2.84	3.73	1.86
Methanosarcina	14.94	3.14	3.06	4.42	4.63	3.40	6.30	2.05
Methanomassiliicoccus	4.13	2.42	6.98	4.81	7.29	2.58	5.65	2.84

菌属	相对丰度（50mg·L^-1 2，4-DCP）/%				相对丰度（100mg·L^-1 2，4-DCP）/%
菌属	R1	R2	R3	R4	R1	R2	R3	R4
Methanothrix	43.24	25.74	33.63	34.64	32.74	20.06	38.17	36.32
Methanobacterium	34.72	61.63	50.26	50.49	45.46	67.02	41.20	49.29
Methanocorpusculum	0.62	5.83	5.34	4.91	4.23	2.84	3.73	1.86
Methanosarcina	14.94	3.14	3.06	4.42	4.63	3.40	6.30	2.05
Methanomassiliicoccus	4.13	2.42	6.98	4.81	7.29	2.58	5.65	2.84

菌属	相对丰度（50mg·L^-1 2，4-DCP）/%				相对丰度（100mg·L^-1 2，4-DCP）/%
菌属	R1	R2	R3	R4	R1	R2	R3	R4
Desulfovibrio	8.99	12.97	11.34	13.63	3.21	8.61	5.87	9.28
Petrimonas	2.10	1.61	7.17	5.57	0.92	0.88	4.07	1.50
Cloacibacillus	1.50	4.95	4.84	5.82	1.51	1.77	0.88	1.02
Mesotoga	0.32	1.87	3.65	2.05	1.58	3.22	12.34	14.54
Syntrophorhabdus	1.42	0.80	0.33	0.36	2.19	0.65	0.17	0.40
Longilinea	0.22	0.07	0.02	0.33	0.22	0.08	0.03	0.36
Leptolinea	0.05	0.17	0.23	0.27	0.01	0.11	0.10	0.12
Ignavibacterium	0.04	0.07	0.06	0.06	0.12	0.59	0.32	1.24

菌属	相对丰度（50mg·L^-1 2，4-DCP）/%				相对丰度（100mg·L^-1 2，4-DCP）/%
菌属	R1	R2	R3	R4	R1	R2	R3	R4
Desulfovibrio	8.99	12.97	11.34	13.63	3.21	8.61	5.87	9.28
Petrimonas	2.10	1.61	7.17	5.57	0.92	0.88	4.07	1.50
Cloacibacillus	1.50	4.95	4.84	5.82	1.51	1.77	0.88	1.02
Mesotoga	0.32	1.87	3.65	2.05	1.58	3.22	12.34	14.54
Syntrophorhabdus	1.42	0.80	0.33	0.36	2.19	0.65	0.17	0.40
Longilinea	0.22	0.07	0.02	0.33	0.22	0.08	0.03	0.36
Leptolinea	0.05	0.17	0.23	0.27	0.01	0.11	0.10	0.12
Ignavibacterium	0.04	0.07	0.06	0.06	0.12	0.59	0.32	1.24

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