Abstract:

Temperature was one of the important parameters which can significantly effect on the activity,dominant species and reactor performance. Anaerobic activated sludge was used as the inoculant and glucose was used as carbon source. The anode biofilm of the single chamber microbial electrolysis cells (MECs) was operated at different temperature. The experimental results showed that the maximum current densities were 2.04A/m², 7.75A/m², 12.27A/m² and 2.5A/m² respectively at 25℃, 30℃, 35℃ and 45℃. The trend of electrochemical activity and hydrogen yield of anode biofilm is similar to that of MECs, which shows that increasing temperature in a certain temperature range is beneficial to the electrochemical activity of anode biofilm and hydrogen yield of anode biofilm. The results of anode biomass and extracellular polymeric substances (EPS) analysis indicated that the increase of temperature within a certain temperature range was beneficial to the increase of the anode membrane biomass. A large number of bacteria adhering to the anode could produce higher current density and EPS content. The protein composition of EPS was significantly higher than that of polysaccharide, and the protein content increased with the increase of current density. Fourier transform infrared (FTIR) spectrophotometry analysis confirmed the presence of proteins and carbohydrates in the biofilm.

Key words: microbiological electrolytic cell (MEC), anode biofilm, temperature, hydrogen production, extracellular polymeric substances (EPS)

摘要：

温度是影响微生物活性和优势菌种以及反应器性能的重要参数之一。实验采用单池微生物电解池（MECs），以厌氧活性污泥为接种物，葡萄糖为碳源，在不同温度条件下培养运行微生物电解池阳极生物膜。实验结果表明：在25℃、30℃、35℃和45℃的培养温度下最大电流密度分别达到2.04A/m²、7.75A/m²、12.27A/m²和2.5A/m²。阳极生物膜电化学活性和氢气产率变化趋势与MECs电流密度相类似，均表现出在一定温度范围内升高温度有利于阳极膜电化学活性与阳极膜氢气产率。进一步分析阳极膜生物量与胞外聚合物（EPS）成分结果表明：一定温度范围内升高温度有利于阳极膜生物量的提高，大量的阳极附着细菌可以产生更高的电流密度与EPS含量，阳极膜EPS中蛋白质成分明显高于多糖，并且蛋白质含量随产电密度增高而增大。傅里叶变换红外光谱（FTIR）分析证实了生物膜EPS中存在蛋白质和碳水化合物。

关键词: 微生物电解池, 阳极生物膜, 温度, 制氢, 胞外聚合物