Separation and biodegradation characteristics of a halotolerant strain

doi:10.16085/j.issn.1000-6613.2017.03.037

Abstract

Abstract:

Coal chemical industry wastewater is a kind of refractory industry wastewater with high salt content. In order to improve biodegradation efficiency of coal chemical industry wastewater,a halotolerant bacteria CCZU-R6 was isolated from the sewage of a coal chemical enterprise. It was identified as Bacillus sp. by its morphological properties and 16S rDNA sequence analysis. Using the method of single factor experiment,the effects of degradation conditions were studied. The optimum treatment conditions for simulated saline wastewater were pH 7.0,temperature 35℃,inoculum concentration 500mg/L,and the maximum salinity tolerance was about 11%. Substrate spectral analysis showed that CCZU-R6 could degrade phenol,benzyl alcohol,and methanol. The COD removal rate was up to 93% when the coal chemical industry saline wastewater was treated by CCZU-R6 for 24 hours under the optimal conditions. The results showed that CCZU-R6 could effectively degrade saline wastewater in coal chemical industry,with good commercial application potential.

Key words: halotolerant strain, separation, degradation, saline wastewater

摘要：

煤化工废水是一种含盐量较高的难降解工业废水，为了提高其生化处理的效果，从某煤化工企业生产污水中分离得到一株耐盐菌CCZU-R6，经形态观察和16S rDNA序列分析，鉴定该菌株属于芽孢杆菌属（Bacillus sp.）。采用单因素实验方法考察了pH、温度、接种量、废水盐度等工艺条件对降解模拟含盐废水的影响，确定了菌株CCZU-R6处理模拟含盐废水的最适条件为pH 7.0、温度35℃，接种量500mg/L，能耐受的最高盐度约为11%。底物谱分析显示菌株CCZU-R6对苯酚、苯甲醇、甲醇等物质具有显著的降解效果。在最适降解条件下，菌株CCZU-R6处理实际煤化工含盐废水24h，COD去除率达到93%。表明该菌株对含盐煤化工废水有较强的降解能力，具有工业应用前景。

关键词: 耐盐菌, 分离, 降解, 含盐废水

CLC Number:

X703
X172

WANG Lijuan, QIAN Ziwen, SHEN Haibo, ZHU Jun, WU Yanfei, CHEN Mengjun, WANG Liqun. Separation and biodegradation characteristics of a halotolerant strain[J]. Chemical Industry and Engineering Progress, 2017, 36(03): 1047-1051.

王丽娟, 钱子雯, 沈海波, 朱君, 吴彦霏, 陈孟君, 王利群. 一株耐盐菌的分离及其降解特性[J]. 化工进展, 2017, 36(03): 1047-1051.

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