Abstract: A lipase-producing strain of CF-12，which could used as the selective kinetic resolution of (R,S)-2-hydroxy-4-Ethyl benzene [(R,S)-HPBE]，was isolated from a variety of rich oil soil. It was identified as Serratia sp. by its morphological，physiological properties and 16S rDNA sequence analysis. The preferred carbon source，nitrogen source and metal ion were sucrose（5 g/L），yeast powder（10 g/L），and Mg²⁺（0.75 mmol/L），respectively. After 24 h cultivation，the lipase activity was 20.1 U/mL. The optimal reaction temperature and pH were 40 ℃ and 7.0，respectively. After 15 h resolution by crude enzyme powder，(R)-HPBE could be obtained with the yield of 39.6% and e.e. of 90.8%.

Key words: lipase, Serratia sp., ethyl (R)-2-hydroxy-4-phenylbutyrate, catalytic characteristics, identification

摘要： 从各种含油脂的土壤中筛选分离到一株高选择性拆分(R,S)-2-羟基-4-苯基丁酸乙酯[(R,S)-HPBE]的产脂肪酶菌株CF-12，经形态观察、生理生化试验和16S rDNA序列分析，鉴定该菌为沙雷氏菌属（Serratia sp.）。对菌株CF-12产脂肪酶的发酵条件进行了初步优化，确定最适产酶条件为：蔗糖5 g/L，酵母粉10 g/L，金属离子Mg²⁺ 0.75 mmol/L，发酵培养24 h，脂肪酶活性可达20.1 U/mL。该脂肪酶最适反应温度和pH值分别为40 ℃和7.0。利用冻干酶粉拆分 (R,S)-HPBE 15 h，(R)-HPBE产率达39.6%，ee=90.8%。

关键词: 脂肪酶, 沙雷氏菌, (R)-2-羟基-4-苯基丁酸乙酯, 催化特性, 鉴定