Synthesis of a novel and non-natural phospholipid: phosphatidylbutyric acid

doi:10.16085/j.issn.1000-6613.2017-2409

Chemical Industry and Engineering Progress ›› 2018, Vol. 37 ›› Issue (10): 4029-4032.DOI: 10.16085/j.issn.1000-6613.2017-2409

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Synthesis of a novel and non-natural phospholipid: phosphatidylbutyric acid

LI Huanyu¹, LI Binglin¹, WANG Jiao¹, DUAN Dandan¹, ZHANG Xiaoli², ZHAO Binxia¹

1 College of Chemical Engeering, Northwest University, Xi'an 710069, Shaanxi, China;
2 College of Food Science and Technology, Northwest University, Xi'an 710069, Shaanxi, China

Received:2017-11-22 Revised:2017-12-19 Online:2018-10-05 Published:2018-10-05

非天然磷脂化合物磷脂酰基γ-羟基丁酸的合成

李环宇¹, 李冰麟¹, 王姣¹, 段丹丹¹, 张小里², 赵彬侠¹

1 西北大学化工学院, 陕西西安 710069;
2 西北大学食品科学与工程学院, 陕西西安 710069

通讯作者: 张小里,教授,博士生导师,研究方向为生物催化过程。
作者简介:李环宇(1993-),女,硕士研究生,研究方向为生物催化过程。
基金资助:
陕西省重点研发计划项目（2018NY-131）。

Abstract

Abstract: The non-natural phosphatidylbutyric acid (PB) was synthesized for the first time using the enzyme-catalyzed method. It was synthesized by phospholipase D (PLD)-catalyzed transphosphatidylation of phosphatidylcholine (PC) with sodium γ-hydroxybutyrate in the liquid-liquid system. The operational conditions of transphosphatidylation were investigated systematically, including organic solvents, molar ratios of sodium γ-hydroxybutyrate to PC, temperature, pH, and the reaction time. The optimal reaction conditions were that:the best candidate of organic solvent was trichloromethane, the molar ratio of Sodium γ-Hydroxybutyrate to PC was 90:1, the reaction temperature was 30℃, pH was 5.5, and the reaction time was 6h. Under those optimal conditions, the highest yield of PB was 73.15%.

Key words: phosphatidylbutyric acid, sodium γ-hydroxybutyrate, phospholipase D, transphosphatidylation

摘要： 首次利用生物酶催化法对非天然磷脂化合物磷脂酰基γ-羟基丁酸（phosphatidylbutyric acid，PB）的合成工艺进行了系统的探究。采用磷脂酶D （phospholipase D，PLD）在有机溶媒-水两相体系中催化磷脂酰胆碱（phosphatidylcholine，PC）和γ-羟基丁酸钠（sodium γ-hydroxybutyrate）发生磷脂酰基转移反应合成PB。实验结果表明，有机溶媒、底物摩尔比、反应温度、水相pH都对磷脂酰基γ-羟基丁酸的制备有一定的影响。确定最佳工艺为：以氯仿为有机溶媒，底物摩尔比（γ-羟基丁酸钠/PC）为90：1，反应温度为30℃，水相pH为5.5，反应时间为6h，在此工艺条件下的磷脂酰基γ-羟基丁酸的产率为73.51%。

关键词: 磷脂酰基γ-羟基丁酸, γ-羟基丁酸钠, 磷脂酶D, 磷脂酰基转移

CLC Number:

TQR971⁺.2

LI Huanyu, LI Binglin, WANG Jiao, DUAN Dandan, ZHANG Xiaoli, ZHAO Binxia. Synthesis of a novel and non-natural phospholipid: phosphatidylbutyric acid[J]. Chemical Industry and Engineering Progress, 2018, 37(10): 4029-4032.

李环宇, 李冰麟, 王姣, 段丹丹, 张小里, 赵彬侠. 非天然磷脂化合物磷脂酰基γ-羟基丁酸的合成[J]. 化工进展, 2018, 37(10): 4029-4032.

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Synthesis of a novel and non-natural phospholipid: phosphatidylbutyric acid

非天然磷脂化合物磷脂酰基γ-羟基丁酸的合成

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