Nano encapsulation and application of epigallocatechin gallate

doi:10.16085/j.issn.1000-6613.2024-1973

Abstract

Abstract:

This study utilized cassava starch as the raw material and employed an ultrasound-microwave-assisted alcohol precipitation method to prepare epigallocatechin gallate (EGCG) nanostarch particles (EGCG-SNPs). The method for the simultaneous nanofication and encapsulation of EGCG was introduced, and the physicochemical properties of EGCG-SNPs were thoroughly characterized using Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (SEM), X-ray powder diffraction (XRD), thermogravimetric analysis (TGA), and laser particle size analysis. The results showed that the EGCG-SNPs exhibited a uniform particle size [(139.10±18.53)nm] and spherical morphology with a wrinkled surface. XRD analysis revealed that the crystalline structure of EGCG-SNPs shifted from the typical A-shape crystalline structure of native cassava starch to a weaker V-shape crystalline structure with a significant reduction in crystallinity and improved thermal stability. FTIR analysis further indicated that the hydrogen bonding interaction between EGCG and starch was enhanced, thereby improving the structural stability of the nanoparticles. The EGCG-SNPs demonstrated a high drug loading capacity [(378.05±3.04)mg/g] and encapsulation efficiency (50.02%±2.22%) and exhibited significant antioxidant activity in DPPH and ABTS free radical scavenging assays. Drug release experiments found that EGCG-SNPs exhibited sustained release behavior in simulated digestive environments. In conclusion, EGCG-SNPs not only exhibited excellent encapsulation efficiency and sustained release properties but also demonstrated stable antioxidant activity, significantly enhancing the therapeutic effect compared to free EGCG. This study provided new insights into the application of EGCG in drug delivery, cancer therapy and the prevention of related diseases.

Key words: nanotechnology, epigallocatechin gallate, simultaneous encapsulation, drug release, starch

摘要：

利用木薯淀粉为原料，采用超声微波辅助醇沉法制备表没食子儿茶素没食子酸酯（EGCG）纳米淀粉颗粒（EGCG-SNPs）。介绍了EGCG的同步纳米化封装方法，并使用傅里叶变换红外光谱（FTIR）、场发射扫描电子显微镜（SEM）、X射线粉末衍射（XRD）、热重分析（TGA）和激光粒度仪等技术对EGCG-SNPs的物理化学性质进行了分析。研究结果表明，EGCG-SNPs具有均匀的粒径[(139.10±18.53)nm]和球形结构，表面具有轮纹。XRD分析显示其晶型由木薯淀粉的A形结构转变为V形结构，结晶度显著降低，热稳定性得到改善。FTIR分析进一步表明EGCG与淀粉之间的氢键作用增强，提升了纳米颗粒的结构稳定性。EGCG-SNPs具有较高的载药量[（378.05±3.04）mg/g]和包埋率（50.02%±2.22%），并在DPPH和ABTS自由基清除实验中表现出显著的抗氧化活性。药物释放实验表明，EGCG-SNPs在模拟消化环境中表现出较好的缓释性能。综上所述，EGCG-SNPs不仅具有良好的包埋效果和缓释性能，还表现出稳定的抗氧化活性，相比游离EGCG，EGCG-SNPs药效显著提高。本文为EGCG在药物传递、癌症治疗及相关疾病防治中的应用提供了新的思路。

关键词: 纳米技术, 表没食子儿茶素没食子酸酯, 同步封装, 药物缓释, 淀粉

CLC Number:

TQ07

CHEN Xiaozhen, SU Yanlei, LU Xinglei, FENG Jie, ZHOU Kaidi, GUAN Xin, LAN Lihong, LAN Ping, HE Rimei. Nano encapsulation and application of epigallocatechin gallate[J]. Chemical Industry and Engineering Progress, 2025, 44(12): 7152-7164.

陈晓真, 苏艳蕾, 陆兴蕾, 冯杰, 周凯迪, 关欣, 蓝丽红, 蓝平, 何日梅. 表没食子儿茶素没食子酸酯的纳米化封装及应用[J]. 化工进展, 2025, 44(12): 7152-7164.

Figures/Tables 12

References 45

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样品	粒径/nm	分散系数/%	zeta电位/mV
SNPs	64.62±2.25	0.45±0.04	-5.46±0.37
EGCG-SNPs	139.10±18.53	0.22±0.03	-11.63±0.25

样品	粒径/nm	分散系数/%	zeta电位/mV
SNPs	64.62±2.25	0.45±0.04	-5.46±0.37
EGCG-SNPs	139.10±18.53	0.22±0.03	-11.63±0.25

样品名称	模型	回归方程	R²
EGCG-SNPs胰液	零级药动学	y=3.68x +1.54	0.9847
	一级药动学	y=-18936.21(1-e^2.13^x )	0.9696
	Higuchi药动学	y=15.36x^1/2-13.87	0.9358
	Ritger-peppas药动学	y=4.531x^0.93	0.9814
EGCG-SNPs胃液	零级药动学	y=4.00 x-6.14	0.9906
	一级药动学	y=18498.17(1-e^-0.000064^x )	0.3161
	Higuchi药动学	y=13.17x^1/2-16.78	0.9960
	Ritger-peppas药动学	y=0.50x^2.06	0.8302

样品名称	模型	回归方程	R²
EGCG-SNPs胰液	零级药动学	y=3.68x +1.54	0.9847
	一级药动学	y=-18936.21(1-e^2.13^x )	0.9696
	Higuchi药动学	y=15.36x^1/2-13.87	0.9358
	Ritger-peppas药动学	y=4.531x^0.93	0.9814
EGCG-SNPs胃液	零级药动学	y=4.00 x-6.14	0.9906
	一级药动学	y=18498.17(1-e^-0.000064^x )	0.3161
	Higuchi药动学	y=13.17x^1/2-16.78	0.9960
	Ritger-peppas药动学	y=0.50x^2.06	0.8302