Effectiveness of hydrothermal pretreatment on valeric acid production during food waste fermentation

doi:10.16085/j.issn.1000-6613.2022-1883

Abstract

Abstract:

The production of valeric acid from anaerobic fermentation is currently limited by its low yield, and few studies have been reported on its production from food waste. Hydrothermal pretreatment has attracted widespread attention because it can promote the dissolution and hydrolysis of substrates, does not require the use of chemical reagents, and is simple to operate. In this paper, we investigated the effect of different hydrothermal pretreatment temperatures on the production of valeric acid during food waste fermentation inoculated with distiller yeast (DY) and waste activated sludge (WAS), respectively. The results showed that the yield of valeric acid could reach 16.19g COD/L (DY) and 18.55g COD/L (WAS) under the optimal hydrothermal pretreatment conditions (180°C), which were 3.66 times and 0.74 times higher than those of the blank group, significantly improving the valeric acid production. The metabolic analysis revealed that the hydrothermal pretreatment could increase the production of propionic acid and thus provide sufficient substrate for the production of valeric acid. In addition, the hydrothermal pretreatment effectively regulated the alcohol to acid ratio of the anaerobic fermentation substrate, and weakened the conversion pathway of valeric acid to heptanoic acid, thus leading to the higher accumulation of valeric acid. Microbial community analysis revealed that hydrothermal pretreatment increased the relative abundance of functional valeric acid-producing bacteria including Megasphaera and Dialister. It should be noted that there was no enrichment of functional valeric acid-producing bacteria in the DY-B group, while the relative abundance of Megasphaera and Dialister could reach 13.77% and 2.26% after hydrothermal pretreatment (180℃). Moreover, the relative abundance of Megasphaera in the WAS group was 6.21% and increased to 9.35% after hydrothermal pretreatment (180℃).

Key words: hydrothermal, anaerobic, fermentation, food waste, valeric acid

摘要：

目前，厌氧发酵产戊酸受限于其产率低，且利用餐厨垃圾产戊酸的研究少有报道。水热预处理因其可以促进底物的溶出和水解，并且无需使用化学试剂及具有操作简单等优点而受到广泛的关注。本文研究了不同水热预处理温度下，分别接种酒曲和剩余污泥对餐厨垃圾厌氧发酵产戊酸的影响。结果表明：最佳水热预处理条件（180℃）下，戊酸产量分别可达16.19g COD/L（酒曲组）和18.55g COD/L（剩余污泥组），相比空白组提高了3.66倍和0.74倍，显著提高发酵产戊酸效率。通过代谢分析发现：水热预处理可提高丙酸的产量，为发酵产戊酸提供充足的底物；此外，水热预处理有效调控厌氧发酵底物醇酸比，削弱戊酸产庚酸的转换途径，实现了戊酸的积累。微生物群落分析发现：水热预处理可提高产戊酸功能菌巨球菌属（Megasphaera）和小类杆菌属（Dialister）的相对丰度，酒曲空白组无产戊酸功能菌的富集，水热预处理（180℃）后，Megasphaera和Dialister的相对丰度可达13.77%和2.26%；剩余污泥空白组Megasphaera的相对丰度为6.21%，水热预处理（180℃）后，Megasphaera的相对丰度增加至9.35%。

关键词: 水热, 厌氧, 发酵, 餐厨垃圾, 戊酸

CLC Number:

X705

WANG Xueting, GU Xia, XU Xianbao, ZHAO Lei, XUE Gang, LI Xiang. Effectiveness of hydrothermal pretreatment on valeric acid production during food waste fermentation[J]. Chemical Industry and Engineering Progress, 2023, 42(9): 4994-5002.

王雪婷, 顾霞, 徐先宝, 赵磊, 薛罡, 李响. 水热预处理对餐厨垃圾厌氧发酵产戊酸的影响[J]. 化工进展, 2023, 42(9): 4994-5002.

Figures/Tables 9

References 35

1	陈雨佳. Fe₃O₄介导提升厌氧发酵定向生产奇/偶数碳VFAs效能的研究[D]. 广州: 广州大学, 2022.
	CHEN Yujia. Study on Fe₃O₄ mediated improvement of the efficiency of directional production of odd/even carbon VFAs by anaerobic fermentation[D]. Guangzhou: Guangzhou University, 2022.
2	SHI Binfang, HUANG Jingang, YIN Zhenjiang, et al. Riboflavin boosts fermentative valeric acid generation from waste activated sludge[J]. BioResources, 2020, 15(2): 3962-3969.
3	LANGE Jean-Paul, PRICE Richard, AYOUB Paul M, et al. Valeric biofuels: A platform of cellulosic transportation fuels[J]. Angewandte Chemie International Edition, 2010, 49(26): 4479-4483.
4	易悦, 王慧中, 郑丹, 等. 丁酸和戊酸互营氧化产甲烷微生物学研究进展[J]. 中国沼气, 2017, 35(3): 3-10.
	YI Yue, WANG Huizhong, ZHENG Dan, et al. Microbiology research progress of syntrophic butyrate and valerate oxidization producing methane[J]. China Biogas, 2017, 35(3): 3-10.
5	BISSELINK Roel J M, CROCKATT Marc, ZIJLSTRA Martin, et al. Identification of more benign cathode materials for the electrochemical reduction of levulinic acid to valeric acid[J]. ChemElectroChem, 2019, 6(13): 3285-3290.
6	GRESES Silvia, Elia TOMÁS-PEJÓ, Cristina GONZÁLEZ-FERNÁNDEZ. Food waste valorization into bioenergy and bioproducts through a cascade combination of bioprocesses using anaerobic open mixed cultures[J]. Journal of Cleaner Production, 2022, 372: 133680.
7	张艳艳, 白佳喆, 左剑恶. 己酸菌富集及其利用餐厨垃圾产己酸的研究[J]. 中国环境科学, 2022, 42(6): 2724-2733.
	ZHANG Yanyan, BAI Jiazhe, ZUO Jian’e. Enrichment of caproate bacteria and its application in caproic acid production from food waste[J]. China Environmental Science, 2022, 42(6): 2724-2733.
8	WANG J L, YIN Y N. Biological production of medium-chain carboxylates through chain elongation: An overview[J]. Biotechnology Advances, 2022, 55: 107882.
9	GROOTSCHOLTEN T I M, STEINBUSCH K J J, HAMELERS H V M, et al. High rate heptanoate production from propionate and ethanol using chain elongation[J]. Bioresource Technology, 2013, 136: 715-718.
10	Ramon GANIGUÉ, NAERT Pieter, CANDRY Pieter, et al. Fruity flavors from waste: A novel process to upgrade crude glycerol to ethyl valerate[J]. Bioresource Technology, 2019, 289: 121574.
11	SPIRITO Catherine M, RICHTER Hanno, RABAEY Korneel, et al. Chain elongation in anaerobic reactor microbiomes to recover resources from waste[J]. Current Opinion in Biotechnology, 2014, 27: 115-122.
12	郭志超，徐先宝，徐婷婷，等. 接种不同菌源的餐厨垃圾发酵代谢途径及产己酸效能分析[J]. 环境工程，2021, 39(9): 160-168.
	GUO Zhichao, XU Xianbao, XU Tingting, et al. Analysis on fermentation pathway and caproate production from food waste by different inoculum[J]. Environmental Engineering, 2021, 39(9): 160-168.
13	刘昊鹏. 不同电子供体对微生物碳链延长合成中链脂肪酸的影响及机理研究[D]. 北京: 北京化工大学, 2021.
	LIU Haopeng. The study of medium chain fatty acid prodcution through chain elongation with different electron donor[D]. Beijing: Beijing University of Chemical Technology, 2021.
14	TAHERZADEH Mohammad J, KARIMI Keikhosro. Fermentation inhibitors in ethanol processes and different strategies to reduce their effects[M]//Biofuels. Amsterdam: Elsevier, 2011: 287-311.
15	TAUER Andreas, ELSS Sandra, FRISCHMANN Matthias, et al. Influence of thermally processed carbohydrate/amino acid mixtures on the fermentation by Saccharomyces cerevisiae [J]. Journal of Agricultural and Food Chemistry, 2004, 52(7): 2042-2046.
16	全威. 马铃薯制品中三类美拉德反应危害物的形成及其对健康的影响[D]. 无锡: 江南大学, 2021.
	QUAN Wei. The formation and health effects of three kinds of Maillard reaction harmful products from potato products[D]. Wuxi: Jiangnan University, 2021.
17	VERAS S T S, CAVALCANTE W A, GEHRING T A, et al. Anaerobic production of valeric acid from crude glycerol via chain elongation[J]. International Journal of Environmental Science and Technology, 2020, 17(3): 1847-1858.
18	罗锦. 泔脚废油脂厌氧发酵定向生成奇数碳VFAs的优化及长链脂肪酸的影响[D]. 广州: 广州大学, 2021.
	LUO Jin. Directional optimization of odd-carbon VFAs production from hogwash waste grease by anaerobic fermentation and the effect of long-chain fatty acids[D]. Guangzhou: Guangzhou University, 2021.
19	李琦. 污泥干式厌氧发酵过程中腐殖酸的转化规律及其对产甲烷的影响[D]. 太原: 太原理工大学, 2019.
	LI Qi. The transformation of humic acid in sludge dry anaerobic fermentation and its effect on methane production[D]. Taiyuan: Taiyuan University of Technology, 2019.
20	LI Jun, ZHANG Wenjuan, LI Xiang, et al. Production of lactic acid from thermal pretreated food waste through the fermentation of waste activated sludge: Effects of substrate and thermal pretreatment temperature[J]. Bioresource Technology, 2018, 247: 890-896.
21	COMA M, VILCHEZ-VARGAS R, ROUME H, et al. Product diversity linked to substrate usage in chain elongation by mixed-culture fermentation[J]. Environmental Science & Technology, 2016, 50(12): 6467-6476.
22	YIN Jun, LIU Jiaze, CHEN Ting, et al. Influence of melanoidins on acidogenic fermentation of food waste to produce volatility fatty acids[J]. Bioresource Technology, 2019, 284: 121-127.
23	ANGENENT Largus T, RICHTER Hanno, BUCKEL Wolfgang, et al. Chain elongation with reactor microbiomes: open-culture biotechnology to produce biochemicals[J]. Environmental Science & Technology, 2016, 50(6): 2796-2810.
24	吴清莲. 乙醇和乳酸引导的碳链增长技术生产中链羧酸的研究[D]. 哈尔滨: 哈尔滨工业大学, 2019.
	WU Qinglian. Study on medium chain carboxylic acid production from chain elongation technology induced by ethanol and lactate[D]. Harbin: Harbin Institute of Technology, 2019.
25	WU Qinglian, GUO Wanqian, BAO Xian, et al. Upgrading liquor-making wastewater into medium chain fatty acid: Insights into co-electron donors, key microflora, and energy harvest[J]. Water Research, 2018, 145: 650-659.
26	苑荣雪. 基于剩余污泥厌氧发酵产物合成中链脂肪酸的可行性研究[D]. 上海: 上海交通大学, 2020.
	YUAN Rongxue. Study on medium chain fatty acids biosynthesis base on anaerobic fermentation products of waste activated sludge[D]. Shanghai: Shanghai Jiao Tong University, 2020.
27	XU Xianbao, GU Xia, YE Tingting, et al. Overcoming carboxylic acid inhibition by granular consortia in high-load liquefied food waste fermentation for efficient lactate accumulation[J]. Journal of Cleaner Production, 2022, 369: 133438.
28	DAI Xiaohu, LI Xiaoshuai, ZHANG Dong, et al. Simultaneous enhancement of methane production and methane content in biogas from waste activated sludge and perennial ryegrass anaerobic co-digestion: The effects of pH and C/N ratio[J]. Bioresource Technology, 2016, 216: 323-330.
29	ZHANG Qianqian, ZHAO Xingyu, LI Wenjing, et al. Responses of short-chain fatty acids production to the addition of various biocarriers to sludge anaerobic fermentation[J]. Bioresource Technology, 2020, 304: 122989.
30	WU Qinglian, BAO Xian, GUO Wanqian, et al. Medium chain carboxylic acids production from waste biomass: Current advances and perspectives[J]. Biotechnology Advances, 2019, 37(5): 599-615.
31	Jinghua LYU, GONG Li, CHEN Xingyue, et al. Enhancements of short-chain fatty acids production via anaerobic fermentation of waste activated sludge by the combined use of persulfate and micron-sized magnetite[J]. Bioresource Technology, 2021, 342: 126051.
32	EDER Ana Silvia, MAGRINI Flaviane Eva, SPENGLER Andressa, et al. Comparison of hydrogen and volatile fatty acid production by Bacillus cereus, Enterococcus faecalis and Enterobacter aerogenes singly, in co-cultures or in the bioaugmentation of microbial consortium from sugarcane vinasse[J]. Environmental Technology & Innovation, 2020, 18: 100638.
33	LIN Miao, FENG Limei, CHENG Zhiqiang, et al. Effect of ethanol or lactic acid on volatile fatty acid profile and microbial community in short-term sequentially transfers by ruminal fermented with wheat straw in vitro[J]. Process Biochemistry, 2021, 102: 369-375.
34	DEMICHELIS Francesca, PLEISSNER Daniel, FIORE Silvia, et al. Investigation of food waste valorization through sequential lactic acid fermentative production and anaerobic digestion of fermentation residues[J]. Bioresource Technology, 2017, 241: 508-516.
35	SHE Y C, HONG J M, ZHANG Q, et al. Revealing microbial mechanism associated with volatile fatty acids production in anaerobic acidogenesis of waste activated sludge enhanced by freezing/thawing pretreatment[J]. Bioresource Technology, 2020, 302: 122869.

样品名称	发酵时间/d
样品名称	2	4	6	8	10	12	14
DY-B	—^①	—	—	6.12	6.06	6.48	—
DY-80	—	4.66	2.08	1.90	1.81	1.81	0.23
DY-100	—	0.86	0.74	0.65	0.39	0.00	0.00
DY-120	—	0.14	0.13	0.11	0.00	0.00	0.00
DY-140	1.30	0.77	0.57	0.57	0.50	0.15	0.00
DY-160	—	0.93	0.69	0.72	0.61	0.00	0.00
DY-180	—	0.67	0.65	0.65	0.54	0.00	0.00
WAS-B	1.55	0.84	0.29	0.05	0.00	0.00	0.00
WAS-80	0.42	0.11	0.09	0.00	0.00	0.00	0.00
WAS-100	0.40	0.16	0.11	0.00	0.00	0.00	0.00
WAS-120	0.49	0.57	0.09	0.08	0.05	0.00	0.00
WAS-140	1.04	1.02	0.19	0.16	0.12	0.00	0.00
WAS-160	1.46	0.44	0.28	0.27	0.24	0.00	0.00
WAS-180	—	0.94	0.27	0.27	0.14	0.00	0.00

样品名称	发酵时间/d
样品名称	2	4	6	8	10	12	14
DY-B	—^①	—	—	6.12	6.06	6.48	—
DY-80	—	4.66	2.08	1.90	1.81	1.81	0.23
DY-100	—	0.86	0.74	0.65	0.39	0.00	0.00
DY-120	—	0.14	0.13	0.11	0.00	0.00	0.00
DY-140	1.30	0.77	0.57	0.57	0.50	0.15	0.00
DY-160	—	0.93	0.69	0.72	0.61	0.00	0.00
DY-180	—	0.67	0.65	0.65	0.54	0.00	0.00
WAS-B	1.55	0.84	0.29	0.05	0.00	0.00	0.00
WAS-80	0.42	0.11	0.09	0.00	0.00	0.00	0.00
WAS-100	0.40	0.16	0.11	0.00	0.00	0.00	0.00
WAS-120	0.49	0.57	0.09	0.08	0.05	0.00	0.00
WAS-140	1.04	1.02	0.19	0.16	0.12	0.00	0.00
WAS-160	1.46	0.44	0.28	0.27	0.24	0.00	0.00
WAS-180	—	0.94	0.27	0.27	0.14	0.00	0.00

样品名称	Chao1	Shannon	Simpson
DY-B	555.58	3.87	0.86
DY-100	551.48	3.90	0.83
DY-140	613.44	4.95	0.90
DY-160	556.77	5.33	0.94
DY-180	661.03	4.61	0.87
WAS-B	1817.48	5.55	0.87
WAS-100	1373.34	4.36	0.77
WAS-140	1290.43	4.53	0.73
WAS-160	1071.31	3.07	0.51
WAS-180	978.67	2.77	0.44

样品名称	Chao1	Shannon	Simpson
DY-B	555.58	3.87	0.86
DY-100	551.48	3.90	0.83
DY-140	613.44	4.95	0.90
DY-160	556.77	5.33	0.94
DY-180	661.03	4.61	0.87
WAS-B	1817.48	5.55	0.87
WAS-100	1373.34	4.36	0.77
WAS-140	1290.43	4.53	0.73
WAS-160	1071.31	3.07	0.51
WAS-180	978.67	2.77	0.44

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