关键词: 重组胶原蛋白, 大肠杆菌, 酵母, 发酵, 纯化, 应用

Abstract: Functional proteins are widely used in national defense, medicine and other fields, which are closely related to human life, national security and people's livelihood, and are the key areas of made in China 2025. Collagen is the most abundant protein in human body, which exists in various tissues and organs, such as tendon, cartilage, skin, blood vessels and so on. The source of collagen extracted from animal tissues is limited, the raw material batch is uneven, the target protein is difficult to separate, the reprocess is difficult, and there may be safety hazards. Compared with animal collagen, the recombinant collagen obtained through green bio-manufacturing technology has attracted the attention of scientists at home and abroad due to its unique advantages such as single component, high safety and controllable process. In this review, the biosynthesis and green manufacturing techniques of recombinant collagen were discussed from the construction of expression systems in animals, plants, Escherichia coli and yeast, fermentation regulation and isolation and purification, as well as the application of collagen in bone defects, wound dressings, drug delivery, tendons and cosmetic. The results show that the functional design, yield and purity of recombinant collagen are the basis of its industrial application as biomaterials. Recombinant collagen will play an important role in biomedical field.

Key words: recombinant collagen, Escherichia coli, yeast, fermentation, purification, application