关键词: 全局转录调控工程, 代谢工程, 定向进化, 耐受性

Abstract: Global transcription machinery engineering is an important technical method to reprogram gene transcription to obtain new cell phenotypes. Through DNA recombination, error-prone PCR and other techniques, multiple rounds of mutation modification of transcription elements in cells are carried out to change the conversion efficiency of RNA polymerase and the affinity for promoters. They change the transcription of cells on the whole genomic level, thereby evolving a cell phenotype that meets actual needs. Global transcription machinery engineering can not only rapidly optimize metabolic pathways and increase the yield of target compounds, but also improve stress tolerances. These advantages have been successfully applied to the construction of microbial cell factories for various compounds production. In this article, we mainly review the research progress of using global transcription machinery engineering to improve compound yields and strain tolerance, and clarifies its application prospects.

Key words: global transcription machinery engineering, metabolic engineering, directed evolution, toleration