关键词: 聚酮, 合成生物学, 前体供应, 代谢途径改造, 制造

Abstract: Polyketides have wide range of medicinal activities and extremely high economic value, but how to synthesize polyketides efficiently, economically, green, and environmentally remains a problem that needs to be solved urgently. With the development of synthetic biology, new technologies and strategies are constantly being used in the biomanufacturing of polyketides. This article introduces the key enzymes, precursors, and metabolic pathways in the biomanufacturing of polyketides, and analyzes the CRISPR technology, post-translational modification metabolic engineering to optimize the metabolic regulatory network; remodel and optimize of metabolic pathways by replacing and optimizing promoters; improve the efficiency of polyketide biomanufacturing and the utilization of carbon sources by exploring strategies such as constructing simple and efficient heterologous expression systems. In addition, the latest research progress of synthetic biology in erythromycin, abamectin, spinosyn are summarized. Finally, the problems faced by the biosynthesis of polyketides and possible solutions, such as balancing primary metabolism and secondary metabolism, constructing new and dominant chassis cells, and redesigning and remolding metabolic networks are prospected.

Key words: polyketides, synthetic biology, precursor supply, metabolic pathway modification, manufacture