化工进展 ›› 2019, Vol. 38 ›› Issue (05): 2421-2428.DOI: 10.16085/j.issn.1000-6613.2018-1570

• 生物与医药化工 • 上一篇    下一篇

褪黑素诱导糖蜜废醪液培养单针藻Monoraphidium sp. FXY-10产生物燃料

董训赞(),赵永腾,余旭亚()   

  1. 昆明理工大学生命科学与技术学院,云南 昆明 650500
  • 收稿日期:2018-07-30 修回日期:2018-09-17 出版日期:2019-05-05 发布日期:2019-05-05
  • 通讯作者: 余旭亚
  • 作者简介:<named-content content-type="corresp-name">董训赞</named-content>(1992—),男,硕士研究生,研究方向为生物质能源。E-mail: <email>xunzandong_kmust@163.com</email>。
  • 基金资助:
    国家自然科学基金(21766012,21666012)

Research on the biofuels production of Monoraphidium sp. FXY-10 by combined melatonin and molasses wastewater

Xunzan DONG(),Yongteng ZHAO,Xuya YU()   

  1. Faculty of Life Science and Technology, Kunming University of Science and Technology,Kunming 650500, Yunnan, China
  • Received:2018-07-30 Revised:2018-09-17 Online:2019-05-05 Published:2019-05-05
  • Contact: Xuya YU

摘要:

研究褪黑素对糖蜜酒精废醪液中单针藻Monoraphidium sp. FXY-10生长和油脂积累的影响。结果表明,以糖蜜酒精废醪液为基础培养基,添加10-3μmol/L的褪黑素,微藻中的油脂含量达到68.69%,是对照组的1.15倍,且最高生物量为1.22g/L,相比对照组提高了41.86 %。与对照组相比,藻细胞内蛋白质和碳水化合物含量分别是对照组的0.61和1.41倍。在微藻培养过程中,糖蜜废醪液中的COD、总氮及总磷的清除率分别达到92.33%、90.07%和86.04%。此外,外源褪黑素的添加提高了乙酰辅酶A羧化酶(acetyl coenzyme A carboxylase,ACCase)、苹果酸酶(malic enzyme,ME)的活性,而降低了磷酸烯醇式丙酮酸羧化酶(phosphoenolpyruvate carboxylase,PEPC)的活性。初步技术经济分析表明,1kg单针藻Monoraphidium sp. FXY-10的干燥粉可以制备535.8g生物柴油。研究表明,褪黑素可以促进糖蜜酒精废醪液中微藻的生长和油脂的积累,降低培养成本,为微藻扩大化生产提供了新的理论基础。

关键词: 糖蜜酒精废醪液, 褪黑素, 单针藻Monoraphidium sp. FXY-10, 生物量, 油脂

Abstract:

The effects of melatonin on the biomass and lipid production in Monoraphidium sp. FXY-10 under molasses wastewater were studied. The molasses wastewater was used as a growth media for the cultivation of Monoraphidium sp. FXY-10. Lipid content of algae was reached 68.69 % with 10-3μmol/L melatonin-treated, which was increased by 1.15-fold. The biomass was 1.22g/L, representing 41.86% higher than that of single molasses wastewater. And the protein and carbohydrate contents were increased and decreased by 1.41- and 0.61-fold, respectively. The removal rate of COD, total nitrogen and total phosphorus in molasses waste mash reached 92.33%, 90.07% and 86.04%, respectively in the microalgae cultivation. Furthermore, the lipid biosynthesis-related enzyme activities correlated with the increased lipid accumulation. The ACCase, and ME activities were considerably up-regulated with PEPC activity but were significantly down-regulated by melatonin induction. The yield analysis indicated that 1kg dried biomass of Monoraphidium sp. FXY-10 might produce about 535.8g of biodiesel. These low cost findings demonstrated that exogenous melatonin could promote the biomass and lipid production in microalgae under molasses wastewater, and provide a theoretical basis for the large-scale microalgae cultivation.

Key words: molasses wastewater, melatonin, Monoraphidium sp. FXY-10, biomass, lipid

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