多酶共固定化技术在糖类催化中的研究进展

doi:10.16085/j.issn.1000-6613.2021-0983

化工进展 ›› 2022, Vol. 41 ›› Issue (5): 2636-2648.DOI: 10.16085/j.issn.1000-6613.2021-0983

多酶共固定化技术在糖类催化中的研究进展

唐婷¹(), 周文凤², 王志¹, 朱晨杰², 许敬亮¹, 庄伟²(), 应汉杰², 欧阳平凯²

^1.郑州大学化工学院，生物+联合研究中心，河南郑州 450002
^2.南京工业大学生物与制药工程学院，国家生化工程技术研究中心，江苏南京 211816

收稿日期:2021-05-10 修回日期:2021-06-22 出版日期:2022-05-05 发布日期:2022-05-24
通讯作者: 庄伟
作者简介:唐婷（1994—），女，硕士研究生，研究方向为固定化酶。E-mail：1335772372@qq.com。
基金资助:
国家重点研发计划(2019YFD1101204);国家自然科学基金(21878142);江苏省杰出青年自然科学基金(BK20190035);江苏省重点研发计划(BE2020712);江苏省六大人才高峰项目(SWYY-016)

Advances of multienzymes co-immobilization technology for sugar catalysis

TANG Ting¹(), ZHOU Wenfeng², WANG Zhi¹, ZHU Chenjie², XU Jingliang¹, ZHUANG Wei²(), YING Hanjie², OUYANG Pingkai²

^1.Biology+ Joint Research Centre, Department of Chemical Technology, Zhengzhou University, Zhengzhou 450002, Henan, China
^2.National Engineering Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China

Received:2021-05-10 Revised:2021-06-22 Online:2022-05-05 Published:2022-05-24
Contact: ZHUANG Wei

摘要/Abstract

摘要：

糖类的催化反应对人体生命活动和工业生产具有重要意义。近年来，多酶共固定化技术迅速发展，为糖类的催化反应提供了一个绿色高效的研究工具。本文结合糖类催化中的多酶级联反应，系统阐述了多酶共固定化的方法，包括传统方法和新型方法，并指出了其各自的原理和优缺点。在此基础上，详细介绍了多酶共固定化技术在糖类催化中的应用，如淀粉的水解、纤维素的利用以及功能性糖的合成等，结果表明多酶共固定化技术不仅具有高效催化、稳定性强、易于分离等特性，而且能够充分发挥多酶之间协同催化的优势，极大地促进了糖类催化反应的进行。最后分析了多酶共固定化技术目前存在的问题与挑战，提出了一些解决问题的研究思路和方法，并展望了其发展前景。

关键词: 多酶共固定化, 糖类催化, 级联反应, 协同效应

Abstract:

Apparently, the catalytic reaction of sugar is of great significance to human life activities and industrial production. In recent years, the rapid development of the multienzymes co-immobilization technology provides a green and efficient research tool for the catalytic reaction of sugar. Under this background, combined with the multienzymes cascade reaction in sugar catalysis, this paper systematically expounds the traditional and new methods of multienzymes co-immobilization, and illustrates its respective principles and advantages as well as disadvantages. On this basis, this paper further introduces the wide applications of multienzymes co-immobilization technology in sugar catalysis in detail, such as starch hydrolysis, cellulose utilization and functional sugar synthesis. In accordance with the related research results, it is indicated that the multienzymes co-immobilization technology not only has a series of remarkable characteristics including high efficiency catalysis, strong stability and easy separation, but also can give full play to the advantages of synergistic catalysis among multienzymes, thus greatly promoting the catalytic reaction of sugars. In the last, this paper analyzes some of the problems and challenges facing multienzymes co-immobilization technology, puts forward some research thoughts and methods for solving these problems，and looks forward to its development prospects.

Key words: multienzymes co-immobilization, sugar catalysis, cascade reaction, synergistic effect

中图分类号:

Q814.2

唐婷, 周文凤, 王志, 朱晨杰, 许敬亮, 庄伟, 应汉杰, 欧阳平凯. 多酶共固定化技术在糖类催化中的研究进展[J]. 化工进展, 2022, 41(5): 2636-2648.

TANG Ting, ZHOU Wenfeng, WANG Zhi, ZHU Chenjie, XU Jingliang, ZHUANG Wei, YING Hanjie, OUYANG Pingkai. Advances of multienzymes co-immobilization technology for sugar catalysis[J]. Chemical Industry and Engineering Progress, 2022, 41(5): 2636-2648.

图/表 1

参考文献 82

43	DE SOUZA P M, DE OLIVEIRA MAGALHÃES P. Application of microbial α‍-amylase in industry—A review[J]. Brazilian Journal of Microbiology, 2010, 41(4): 850-861.
44	AKOH C C, CHANG S W, LEE G C, et al. Biocatalysis for the production of industrial products and functional foods from rice and other agricultural produce[J]. Journal of Agricultural and Food Chemistry, 2008, 56(22): 10445-10451.
45	TORABIZADEH H, MONTAZERI E. Nano co-immobilization of α-amylase and maltogenic amylase by nanomagnetic combi-cross-linked enzyme aggregates method for maltose production from corn starch[J]. Carbohydrate Research, 2020, 488: 107904.
46	TALEKAR S, DESAI S, PILLAI M, et al. Carrier free co-immobilization of glucoamylase and pullulanase as combi-cross linked enzyme aggregates (combi-CLEAs)[J]. RSC Adv., 2013, 3(7): 2265-2271.
47	ZHANG L, SHI J F, JIANG Z Y, et al. Bioinspired preparation of polydopamine microcapsule for multienzyme system construction[J]. Green Chem., 2011, 13(2): 300-306.
48	SALGAONKAR M, NADAR S S, RATHOD V K. Combi-metal organic framework (Combi-MOF) of α‍-amylase and glucoamylase for one pot starch hydrolysis[J]. International Journal of Biological Macromolecules, 2018, 113: 464-475.
49	HAN P, ZHOU X, YOU C. Efficient multi-enzymes immobilized on porous microspheres for producing inositol from starch[J]. Frontiers in Bioengineering and Biotechnology, 2020, 8: 380.
50	DAI Z F. Co-immobilization of thermostable alpha-amylase and glucoamylase for starch hydrolysis[D]. Columbus: Ohio State University, 2011.
51	LYND L R, WEIMER P J, VAN ZYL W H, et al. Microbial cellulose utilization: fundamentals and biotechnology[J]. Microbiology and Molecular Biology Reviews, 2002, 66(3): 506-577.
52	CHO E J, JUNG S, KIM H J, et al. Co-immobilization of three cellulases on Au-doped magnetic silicananoparticles for the degradation of cellulose[J]. ChemCommun, 2012, 48(6): 886-888.
53	YUAN B, YANG X Q, XUE L W, et al. A novel recycling system for nano-magnetic molecular imprinting immobilised cellulases: synergistic recovery of anthocyanin from fruit and vegetable waste[J]. Bioresource Technology, 2016, 222: 14-23.
54	MULEY A B, THORAT A S, SINGHAL R S, et al. A tri-enzyme co-immobilized magnetic complex: process details, kinetics, thermodynamics and applications[J]. International Journal of Biological Macromolecules, 2018, 118: 1781-1795.
1	RAUTER A P, XAVIER N M. Special issue “carbohydrates 2018”[J]. Pharmaceuticals, 2019, 13(1): E5.
2	CUMMINGS J H, STEPHEN A M. Carbohydrate terminology and classification[J]. European Journal of Clinical Nutrition, 2007, 61(S1): S5-S18.
3	WANG M, LIU M J, LU J M, et al. Photo splitting of bio-polyols and sugars to methanol and syngas[J]. Nature Communications, 2020, 11: 1083.
4	RODRIGUES R C, ORTIZ C, BERENGUER-MURCIA Á, et al. Modifying enzyme activity and selectivity by immobilization[J]. Chemical Society Reviews, 2013, 42(15): 6290-6307.
5	ZHANG H, BAI Y P, ZHU N, et al. Microfluidic reactor with immobilized enzyme-from construction to applications: a review[J]. Chinese Journal of Chemical Engineering, 2021, 30: 136-145.
6	CHEN K, HUANG X Y, KAN S B J, et al. Enzymatic construction of highly strained carbocycles[J]. Science, 2018, 360(6384): 71-75.
7	CUI J D, REN S Z, LIN T, et al. Shielding eﬀects of Fe³⁺-tannic acid nanocoatings for immobilized enzyme on magnetic Fe₃O₄@silica core shell nanosphere[J]. Chemical Engineering Journal, 2008, 343: 629-637.
8	CUI J, FENG Y, LIN T, et al. Mesoporous metal-organic framework with well-defined cruciate flower-like morphology for enzyme immobilization[J]. ACS Applied Materials & Interfaces, 2017, 9(12): 10587-10594.
9	NESTL B M, HAMMER S C, NEBEL B A, et al. New generation of biocatalysts for organic synthesis[J]. Angewandte Chemie International Edition, 2014, 53(12): 3070-3095.
10	JO S M, WURM F R, LANDFESTER K. Biomimetic cascade network between interactive multi-compartments organized by enzyme-loaded silica nanoreactors[J]. ACS Applied Materials and Interfaces, 2018, 10(40): 34230-34237.
11	BELLUATI A, CRACIUN I, LIU J, et al. Nanoscale enzymatic compartments in tandem support cascade reactions in vitro [J]. Biomacromolecules, 2018, 19(10): 4023-4033.
12	REN S Z, LI C H, JIAO X B, et al. Recent progress in multienzymes co-immobilization and multienzyme system applications[J]. Chemical Engineering Journal, 2019, 373: 1254-1278.
13	YIN J F, CHEN S K, ZHANG N, et al. Multienzyme cascade bioreactor for a 10min digestion of genomic DNA into single nucleosides and quantitative detection of structural DNA modifications in cellular genomic DNA[J]. ACS Applied Materials & Interfaces, 2018, 10(26): 21883-21890.
14	SCHOFFELEN S, HEST J C M VAN. Multi-enzyme systems: bringing enzymes together in vitro [J]. Soft Matter, 2012, 8(6): 1736-1746.
15	ZHANG Y H P. Simpler is better: high-yield and potential low-cost biofuels production through cell-free synthetic pathway biotransformation (SyPaB)[J]. ACS Catalysis, 2011, 1(9): 998-1009.
16	HWANG E T, LEE S. Multienzymatic cascade reactions via enzyme complex by immobilization (review)[J]. ACS Catalysis, 2019, 9(5): 4402-4425.
17	郭华, 张蕾, 董旭, 等. 固定化多酶级联反应器[J]. 化学进展, 2020, 32(4): 392-405.
	GUO Hua, ZHANG Lei, DONG Xu, et al. Immobilized multi-enzyme cascade reactor[J]. Progress in Chemistry, 2020, 32(4): 392-405.
18	ULKER C, GOKALP N, GUVENILIR Y. Immobilization of Candida Antarctica lipase B (CALB) on surface-modified rice husk ashes (RHA) via physical adsorption and cross-linking methods[J]. Biocatalysis and Biotransformation, 2016, 34(4): 172-180.
19	GASHTASBI F, AHMADIAN G, NOGHABI K A. New insights into the effectiveness of alpha-amylase enzyme presentation on the Bacillus subtilis spore surface by adsorption and covalent immobilization[J]. Enzyme and Microbial Technology, 2014, 64/65: 17-23.
20	CIAURRIZ P, BRAVO E, HAMAD-SCHIFFERLI K. Effect of architecture on the activity of glucose oxidase/horseradish peroxidase/carbon nanoparticle conjugates[J]. Journal of Colloid and Interface Science, 2014, 414: 73-81.
21	QU R, SHEN L L, QU A T, et al. Artificial peroxidase/oxidase multiple enzyme system based on supramolecular hydrogel and its application as a biocatalyst for cascade reactions[J]. ACS Applied Materials & Interfaces, 2015, 7(30): 16694-16705.
55	KUMARI A, KAILA P, TIWARI P, et al. Multiple thermostable enzyme hydrolases on magnetic nanoparticles: an immobilized enzyme-mediated approach to saccharification through simultaneous xylanase, cellulase and amylolytic glucanotransferase action[J]. International Journal of Biological Macromolecules, 2018, 120: 1650-1658.
56	张群. 功能性糖生物制备关键技术研究[J]. 食品与生物技术学报, 2018, 37(9): 1008.
	ZHANG Qun. Study on the key technology of biological preparation of functional sugar[J]. Journal of Food Science and Biotechnology, 2018, 37(9): 1008.
57	ZHAO C, WU Y J, LIU X Y, et al. Functional properties, structural studies and chemo-enzymatic synthesis of oligosaccharides[J]. Trends in Food Science & Technology, 2017, 66: 135-145.
58	SAKO T, MATSUMOTO K, TANAKA R. Recent progress on research and applications of non-digestible galacto-oligosaccharides[J]. International Dairy Journal, 1999, 9(1): 69-80.
59	LI Z Y, XIAO M, LU L L, et al. Production of non-monosaccharide and high-purity galactooligosaccharides by immobilized enzyme catalysis and fermentation with immobilized yeast cells[J]. Process Biochemistry, 2008, 43(8): 896-899.
60	ABURTO C, GUERRERO C, VERA C, et al. Co-immobilized β‍-galactosidase and Saccharomyces cerevisiae cells for the simultaneous synthesis and purification of galacto-oligosaccharides[J]. Enzyme and Microbial Technology, 2018, 118: 102-108.
61	MARÍN-MANZANO M C, ABECIA L, HERNÁNDEZ-HERNÁNDEZ O, et al. Galacto-oligosaccharides derived from lactulose exert a selective stimulation on the growth of bifidobacterium animalis in the large intestine of growing rats[J]. Journal of Agricultural and Food Chemistry, 2013, 61(31): 7560-7567.
62	LONG J, PAN T, XIE Z J, et al. Effective production of lactosucrose using β‍-fructofuranosidase and glucose oxidase co-immobilized by sol-gel encapsulation[J]. Food Science & Nutrition, 2019, 7(10): 3302-3316.
63	ÖLÇER Z, TANRISEVEN A. Co-immobilization of dextransucrase and dextranase in alginate[J]. Process Biochemistry, 2010, 45(10): 1645-1651.
64	MURANAKA Y, MATSUBARA K, MAKI T, et al. 5-Hydroxymethylfurfural synthesis from monosaccharides by a biphasic reaction-extraction system using a microreactor and extractor[J]. ACS Omega, 2020, 5(16): 9384-9390.
65	千嘉艺, 肖建军, 孙林, 等. 生物质双相溶剂体系制备5-羟甲基糠醛的过程强化研究进展[J]. 化工进展, 2021, 40(11): 6054-6060.
	QIAN Jiayi, XIAO Jianjun, SUN Lin, et al. Research progress on process intensification in hydrolysis of biomass into 5-hydroxymethylfurfural in biphasic solvent systems[J]. Chemical Industry and Engineering Progress, 2021, 40(11): 6054-6060.
66	WU Z F, SHI L J, YU X X, et al. Co-immobilization of tri-enzymes for the conversion of hydroxymethylfurfural to 2,‍5-diformylfuran[J]. Molecules, 2019, 24(20): 3648.
67	RAMACHANDRAN S, FONTANILLE P, PANDEY A, et al. Gluconic acid: properties, applications and microbial production[J]. Food Technology and Biotechnology, 2006, 44(2): 185-195.
68	LIU F J, XUE Z M, ZHAO X H, et al. Catalytic deep eutectic solvents for highly efficient conversion of cellulose to gluconic acid with gluconic acid self-precipitation separation[J]. Chemical Communications, 2018, 54(48): 6140-6143.
69	RUALES-SALCEDO A V, HIGUITA J C, FONTALVO J. Integration of a multi-enzyme system with a liquid membrane in Taylor flow regime for the production and in situ recovery of gluconic acid from cellulose[J]. Chemical Engineering and Processing-Process Intensification, 2020, 157: 108140.
70	HAN X L, LIU G D, SONG W X, et al. Production of sodium gluconate from delignified corn cob residue by on-site produced cellulase and co-immobilized glucose oxidase and catalase[J]. Bioresource Technology, 2018, 248: 248-257.
71	YU X X, ZHANG Z Y, LI J Z, et al. Co-immobilization of multi-enzyme on reversibly soluble polymers in cascade catalysis for the one-pot conversion of gluconic acid from corn straw[J]. Bioresource Technology, 2021, 321: 124509.
72	BACHOSZ K, SYNORADZKI K, STASZAK M, et al. Bioconversion of xylose to xylonic acid via co-immobilized dehydrogenases for conjunct cofactor regeneration[J]. Bioorganic Chemistry, 2019, 93: 102747.
73	LE DARÉ B, GICQUEL T. Therapeutic applications of ethanol: a review[J]. Journal of Pharmacy & Pharmaceutical Sciences, 2019, 22(1): 525-535.
74	HANSEN A C, ZHANG Q, LYNE P W L. Ethanol-diesel fuel blends—A review[J]. Bioresource Technology, 2005, 96(3): 277-285.
22	SILVA R M DA, PAIVA SOUZA P M, FERNANDES F A N, et al. Co-immobilization of dextransucrase and dextranase in epoxy-agarose- tailoring oligosaccharides synthesis[J]. Process Biochemistry, 2019, 78: 71-81.
23	TALEKAR S, PANDHARBALE A, LADOLE M, et al. Carrier free co-immobilization of alpha amylase, glucoamylase and pullulanase as combined cross-linked enzyme aggregates (combi-CLEAs): a tri-enzyme biocatalyst with one pot starch hydrolytic activity[J]. Bioresource Technology, 2013, 147: 269-275.
24	GARCIA-GALAN C, BERENGUER-MURCIA Á, FERNANDEZ-LAFUENTE R, et al. ChemInform abstract: potential of different enzyme immobilization strategies to improve enzyme performance[J]. Advanced Synthesis & Catalysis, 2012, 353(16): 2885-2904.
25	王金丹, 张光亚. 多酶共固定化的研究进展[J]. 生物工程学报, 2015, 31(4): 469-480.
	WANG Jindan, ZHANG Guangya. Progress in co-immobilization of multiple enzymes[J]. Chinese Journal of Biotechnology, 2015, 31(4): 469-480.
26	WU J, FILUTOWICZ M. Hexahistidine (His6)-tag dependent protein dimerization: a cautionary tale[J]. Acta Biochimica Polonica, 1999, 46(3): 591-599.
27	LIU Z Y, ZHANG J B, CHEN X, et al. Combined biosynthetic pathway for de novo production of UDP-galactose: catalysis with multiple enzymes immobilized on agarose beads[J]. ChemBioChem, 2002, 3(4): 348-355.
28	PLŽ M, PETROVIČOVÁ T, REBROŠ M. Semi-continuous flow biocatalysis with affinity co-immobilized ketoreductase and glucose dehydrogenase[J]. Molecules, 2020, 25(18): 4278.
29	JIA X L, CHEN X, HAN J M, et al. Triple signal amplification using gold nanoparticles, bienzyme and platinum nanoparticles functionalized graphene as enhancers for simultaneous multiple electrochemical immunoassay[J]. Biosensors & Bioelectronics, 2014, 53: 65-70.
30	MANSUR H S, MANSUR A A P, MARQUES M E. Multi-enzymatic systems with designed 3D architectures for constructing food bioanalytical sensors[J]. Food Analytical Methods, 2014, 7(6): 1166-1178.
31	CHEN H, XI F N, GAO X, et al. Bienzyme bionanomultilayer electrode for glucose biosensing based on functional carbon nanotubes and sugar-lectin biospecific interaction[J]. Analytical Biochemistry, 2010, 403(1/2): 36-42.
32	SONG J Y, HE W T, SHEN H, et al. Exquisitely designed magnetic DNA nanocompartment for enzyme immobilization with adjustable catalytic activity and improved enzymatic assay performance[J]. Chemical Engineering Journal, 2020, 390: 124488.
33	YANG Y, ZHANG R Q, ZHOU B N, et al. High activity and convenient ratio control: DNA-directed coimmobilization of multiple enzymes on multifunctionalized magnetic nanoparticles[J]. ACS Applied Materials & Interfaces, 2017, 9(42): 37254-37263.
34	ROTHEMUND P W K. Folding DNA to create nanoscale shapes and patterns[J]. Nature, 2006, 440(7082): 297-302.
35	LIU Y, DU J J, YAN M, et al. Biomimetic enzyme nanocomplexes and their use as antidotes and preventive measures for alcohol intoxication[J]. Nature Nanotechnology, 2013, 8(3): 187-192.
36	DEY S, FAN C H, GOTHELF K V, et al. DNA origami[J]. Nature Reviews Methods Primers, 2021, 1(1): 1-24.
37	WILNER O I, WEIZMANN Y, GILL R, et al. Enzyme cascades activated on topologically programmed DNA scaffolds[J]. Nature Nanotechnology, 2009, 4(4): 249-254.
38	FU J L, LIU M H, LIU Y, et al. Interenzyme substrate diffusion for an enzyme cascade organized on spatially addressable DNA nanostructures[J]. Journal of the American Chemical Society, 2012, 134(12): 5516-5519.
39	MARTIN C K A, WAGNER F. Microbial transformation of β‍-sitosterol by Nocardia sp. M 29[J]. European Journal of Applied Microbiology and Biotechnology, 1976, 2(4): 243-255.
40	居乃琥. 酶工程研究和开发的现状与展望[J]. 工业微生物, 1987, 17(6): 23-30.
	JU N H. Current situation and prospect of enzyme engineering research and development[J]. Industrial Microbe Microbiology, 1987, 17(6): 23-30.
41	SCHAFHAGSER D Y, STOREY K B. Fructose production[J]. Applied Biochemistry and Biotechnology, 1992, 36(1): 63-74.
42	SHEN H, SONG J Y, ZHOU Z X, et al. DNA-directed immobilized enzymes on recoverable magnetic nanoparticles shielded in nucleotide coordinated polymers[J]. Industrial & Engineering Chemistry Research, 2019, 58(20): 8585-8596.
75	ALTUNTAŞ E G, Ö; ZÇELIK F. Ethanol production from starch by co-immobilized amyloglucosidase—Zymomonas mobilis cells in a continuously-stirred bioreactor[J]. Biotechnology & Biotechnological Equipment, 2013, 27(1): 3506-3512.
76	SILVA C R, ZANGIROLAMI T C, RODRIGUES J P, et al. An innovative biocatalyst for production of ethanol from xylose in a continuous bioreactor[J]. Enzyme and Microbial Technology, 2012, 50(1): 35-42.
77	LEE S Y, PARK S J. A review on solid adsorbents for carbon dioxide capture[J]. Journal of Industrial and Engineering Chemistry, 2015, 23: 1-11.
78	KUMARAVEL V, BARTLETT J, PILLAI S C. Photoelectrochemical conversion of carbon dioxide (CO₂) into fuels and value-added products[J]. ACS Energy Letters, 2020, 5(2): 486-519.
79	MARPANI F, PINELO M, MEYER A S. Enzymatic conversion of CO₂ to CH₃OH via reverse dehydrogenase cascade biocatalysis: quantitative comparison of efficiencies of immobilized enzyme systems[J]. Biochemical Engineering Journal, 2017, 127: 217-228.
80	MA K, YEHEZKELI O, PARK E, et al. Enzyme mediated increase in methanol production from photoelectrochemical cells and CO₂ [J]. ACS Catalysis, 2016, 6(10): 6982-6986.
81	HUANG W D. Synthesis of sugar and fixation of CO₂ through artificial photosynthesis driving by hydrogen or electricity[EB/OL]. 2011. doi: 10.3969/j.issn.0253-2778.2011.05.013 .
82	ZHOU J H, YU S S, KANG H L, et al. Construction of multi-enzyme cascade biomimetic carbon sequestration system based on photocatalytic coenzyme NADH regeneration[J]. Renewable Energy, 2020, 156: 107-116.

[1]	张耀丹, 孙若溪, 陈鹏程. 以级联反应为基础的多酶共固定载体研究进展[J]. 化工进展, 2023, 42(6): 3167-3176.
[2]	栗童,仲兆平,张波. 纤维素与多氢原料共热解的协同效应[J]. 化工进展, 2019, 38(9): 4044-4051.
[3]	黄传峰, 韩磊, 霍鹏举, 刘树伟, 程秋香. 煤油共炼残渣与榆林煤共热解特性及半焦性质[J]. 化工进展, 2018, 37(S1): 57-62.
[4]	唐思哲, 胡家秀, 赵健, 柯伟, 王维斌. 新型无机复配缓蚀剂对钠基膨润土中Q235钢的缓蚀作用[J]. 化工进展, 2018, 37(12): 4806-4813.
[5]	严平, 占昌朝, 曹小华, 谢宝华, 徐常龙, 张旭. 原位合成H₄SiW₁₂O₄₀@C协同UV/H₂O₂降解罗丹明B模拟废水[J]. 化工进展, 2015, 34(3): 872-878.
[6]	付友思, 吴又多, 陈丽杰. Zn²⁺、Ca²⁺和Mn²⁺对丙酮丁醇发酵的协同影响[J]. 化工进展, 2015, 34(10): 3719-3724.
[7]	冯茹森, 蒲迪, 周洋, 陈俊华, 寇将, 姜雪, 郭拥军. 混合型烷醇酰胺组成对油/水动态界面张力的影响[J]. 化工进展, 2015, 34(08): 2955-2960.
[8]	马缓，齐暑华，张帆，史金玲. 复合填料/聚丙烯酸酯导电压敏胶的制备与性能[J]. 化工进展, 2014, 33(07): 1791-1795.
[9]	薛晓军，贾广信，何俊辉，李婷. 二甲醚与合成气反应制乙醇的热力学计算与分析[J]. 化工进展, 2014, 33(05): 1160-1163.
[10]	陈燕敏，孙彩霞，吴晋英，黄长山. 一种环保型阻垢缓蚀剂的性能[J]. 化工进展, 2014, 33(01): 204-208.
[11]	王俊，史春霞，吕春胜，李杰，张荣明，郭艳东，曲红杰. 新型聚烯烃抗氧剂的协同抗氧化作用 [J]. 化工进展, 2011, 30(7): 1546-.
[12]	黄丽，孙惠惠，王成忠. 含磷阻燃型环氧树脂的研究进展 [J]. 化工进展, 2011, 30(6): 1277-.
[13]	任晓光，谢云峰，宣征南. 复合型缓蚀剂的缓蚀性能 [J]. 化工进展, 2007, 26(4): 577-.

多酶共固定化技术在糖类催化中的研究进展

Advances of multienzymes co-immobilization technology for sugar catalysis

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 1

参考文献 82

相关文章 13

编辑推荐

Metrics

本文评价