负载酶@ZIF-8复合物的聚合物微颗粒可控制备

doi:10.16085/j.issn.1000-6613.2021-0896

化工进展 ›› 2022, Vol. 41 ›› Issue (4): 2022-2028.DOI: 10.16085/j.issn.1000-6613.2021-0896

负载酶@ZIF-8复合物的聚合物微颗粒可控制备

张彦¹(), 汪伟¹^,²(), 谢锐¹^,², 巨晓洁¹^,², 刘壮¹^,², 褚良银¹^,²

^1.四川大学化学工程学院，四川成都 610065
^2.四川大学高分子材料工程国家重点实验室，四川成都 610065

收稿日期:2021-04-27 修回日期:2021-05-13 出版日期:2022-04-23 发布日期:2022-04-25
通讯作者: 汪伟
作者简介:张彦（1996—），男，硕士研究生，研究方向为微流控技术。E-mail：linyiyzhang@163.com。
基金资助:
国家自然科学基金(21922809)

Controllable fabrication of polymeric microparticles loaded with enzyme@ZIF-8

ZHANG Yan¹(), WANG Wei¹^,²(), XIE Rui¹^,², JU Xiaojie¹^,², LIU Zhuang¹^,², CHU Liangyin¹^,²

^1.School of Chemical Engineering, Sichuan University, Chengdu 610065, Sichuan, China
^2.State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, Sichuan, China

Received:2021-04-27 Revised:2021-05-13 Online:2022-04-23 Published:2022-04-25
Contact: WANG Wei

摘要/Abstract

摘要：

通过仿生物矿化法在微流控法制备的均一聚丙烯酰胺-共聚-丙烯酸水凝胶（PAM-AA）微颗粒上原位生长酶@ZIF-8纳米颗粒，本文成功构建了具有优良催化性能、储存性能和环境耐受性能的酶@ZIF-8/PAM-AA微颗粒。该微颗粒的平均粒径为559.97μm，CV值为2.17%，具有良好的单分散性。在该微颗粒中，酶@ZIF-8纳米颗粒主要原位生长于微颗粒的表面，有利于其有效地与底物接触进行催化反应。以过氧化氢酶为典型的酶研究该微颗粒的催化性能发现，该微颗粒在具有良好催化性能的同时还展现出良好的储存性能和环境耐受性。该微颗粒分别经30min的80℃热处理、紫外线照射或胰蛋白酶处理后，仍能保持约75%的相对活性；此外，微颗粒在常温下经过7天后，其酶活性几乎维持不变。该工作为设计和构建具有优良性能的新型酶固定化功能微颗粒提供了一种新策略。

关键词: 酶固定化, 金属有机骨架, 微流控, 水凝胶, 微颗粒, 生物催化

Abstract:

Enzyme@ZIF-8/PAM-AA microparticles with excellent catalytic, storage and environmental tolerance properties, were synthesized by in situ growth of enzyme@ZIF-8 nanoparticles via biomineralization on uniform polyacrylamide-co-acrylic acid hydrogel (PAM-AA) microparticles from microfluidics. The microparticles showed average sizes of 559.97μm, with CV value of 2.17%, exhibiting good monodispersity. The enzyme@ZIF-8 nanoparticles mainly grew in situ on the microparticle surface, which facilitated their effective contact with the substrates for catalytic reaction. The catalytic performance of the microparticles was studied by using hydrogen peroxidase as the typical enzyme. The results showed that, the microparticles exhibited good catalytic performance, as well as good storage properties and environmental tolerance.The microparticles maintained about 75% of relative activity after treatment with 80℃ water, UV irradiation and trypsin for 30 min, respectively. Moreover, their enzymatic activity remained nearly unchanged after stored at room temperature for 7 days. This work provided a new strategy for design and fabrication of novel advanced functional microparticles for enzyme immobilization.

Key words: enzyme immobilization, metal organic frameworks, microfluidics, hydrogel, microparticles, biocatalysis

中图分类号:

TQ317

张彦, 汪伟, 谢锐, 巨晓洁, 刘壮, 褚良银. 负载酶@ZIF-8复合物的聚合物微颗粒可控制备[J]. 化工进展, 2022, 41(4): 2022-2028.

ZHANG Yan, WANG Wei, XIE Rui, JU Xiaojie, LIU Zhuang, CHU Liangyin. Controllable fabrication of polymeric microparticles loaded with enzyme@ZIF-8[J]. Chemical Industry and Engineering Progress, 2022, 41(4): 2022-2028.

图/表 8

图1 酶@ZIF-8/PAM-AA微颗粒的制备过程示意图

图2 W/O乳液液滴及PAM-AA微颗粒的光学显微镜图以及粒径分布图

图3 HRP@ZIF-8/PAM-AA微颗粒的光学显微镜图及粒径分布图

图4 HRP@ZIF-8/PAM-AA微颗粒的SEM图

图5 HRP@ZIF-8/PAM-AA微颗粒的XPS分析图

图6 HRP@ZIF-8/PAM-AA微颗粒的热重分析

图7 含HRP@ZIF-8/PAM-AA微颗粒及PAM-AA微颗粒的反应液在415nm处的吸光度随时间的变化

图8 HRP@ZIF-8/PAM-AA微颗粒的储存性能和环境耐受性能

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负载酶@ZIF-8复合物的聚合物微颗粒可控制备

Controllable fabrication of polymeric microparticles loaded with enzyme@ZIF-8

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