香蕉假茎中产碱性果胶酶细菌的筛选发酵优化与酶学性质

doi:10.16085/j.issn.1000-6613.2015.09.033

化工进展 ›› 2015, Vol. 34 ›› Issue (09): 3415-3420.DOI: 10.16085/j.issn.1000-6613.2015.09.033

香蕉假茎中产碱性果胶酶细菌的筛选发酵优化与酶学性质

刘长高, 何忠平, 张玉苍, 席小勇

海南大学材料与化工学院, 海南海口 570228

收稿日期:2015-01-12 修回日期:2015-02-21 出版日期:2015-09-05 发布日期:2015-09-05
通讯作者: 张玉苍,教授,博士生导师,从事生物质废弃物资源化利用的研究。E-mail:yczhang@hainu.edu。
作者简介:刘长高(1990-),男,硕士研究生。
基金资助:
国家自然科学基金(51263006)、教育部博导类专项基金(20134601110004)、海南省国际科技合作专项(KJHZ2014-02)及海南省自然科学基金(314074)项目。

Isolation,fermentation optimization and enzymatic properties of a pectinase producing strain from banana pesudostem

LIU Changgao, HE Zhongping, ZHANG Yucang, XI Xiaoyong

College of Materials and Chemical Engineering, Hainan University, Haikou 570228, Hainan, China

Received:2015-01-12 Revised:2015-02-21 Online:2015-09-05 Published:2015-09-05

摘要/Abstract

摘要： 利用果胶酶处理天然纤维对环境污染小,然而筛选高产量的菌株仍是降低成本获得果胶酶的关键。本文利用以果胶为唯一碳源的培养基,从香蕉假茎中分离筛选出了一株具有较高果胶酶活性的菌株。经16S rDNA序列分析表明,该菌株与枯草芽孢杆菌(Bacillus substilis)具有99%的相似性,将该菌株命名为Bacillus sp. ZLXH-5。该菌株最佳发酵时间为2天,最佳发酵温度是37℃,最佳初始pH值是5,最佳转速是180r/min,最佳葡萄糖浓度为15g/L。通过发酵条件优化,果胶酶产量提高了56.6%。对粗酶液的性质进行分析,其在55℃下达到最佳反应速率,最适催化pH值是8.5,不同的离子对于果胶酶的活性起到不同的效果。由于该菌株具有较高的果胶酶活性,可以利用该菌株对香蕉假茎进行生物脱胶。

关键词: 果胶酶, 发酵优化, 生物脱胶, 香蕉假茎

Abstract: The use of pectianase in natural fiber processing causes less pollution to environment. The key to get pectinase is to isolate a highly producing pectinase strain. A bacterial strain with high production of pectinase had been isolated and screened from banana pesudostem with a selective medium. The phylogenetic analysis based on 16S rDNA showed that Bacillus sp. ZLXH-5 was close to Bacillus substilis with 99% sequence identity. It was named as Bacillus sp. ZLXH-5. The optimal fermentation condition was as follows,time of 2d,temperature of 37℃,pH value of 5,rotary speed of 180r/min,and glucose concentration of 15g/L. Studies on enzymatic properties showed that optimal temperature for pectinase was 55℃,and optimal pH value for pectinase was 8.5. Different ions showed different impacts on pectinase activity. This strain can be used to process banana pesudostem because of its high pectinase production.

Key words: pectinase, fermentation optimation, bio-degumming, banana pesudostem

中图分类号:

Q939.9

刘长高, 何忠平, 张玉苍, 席小勇. 香蕉假茎中产碱性果胶酶细菌的筛选发酵优化与酶学性质[J]. 化工进展, 2015, 34(09): 3415-3420.

LIU Changgao, HE Zhongping, ZHANG Yucang, XI Xiaoyong. Isolation,fermentation optimization and enzymatic properties of a pectinase producing strain from banana pesudostem[J]. Chemical Industry and Engineering Progree, 2015, 34(09): 3415-3420.

参考文献

[1] Jacob P,Prema P. Novel process for the simultaneous extraction and degumming of banana fibers under solid-state cultivation[J]. Brazilian Journal of Microbiology,2008,39(1):115-121.

[2] Paul S A,Boudenne A,Ibos L,et al. Effect of fiber loading and chemical treatments on thermophysical properties of banana fiber/polypropylene commingled composite materials[J]. Composites Part A:Applied Science and Manufacturing,2008,39(9):1582-1588.

[3] 黄雅婷,何叶丽. 新颖的纺织纤维--香蕉纤维[J]. 印染,2013(21):54-55.

[4] 蔡勇,韦仕岩,吴圣进,等. 香蕉假茎生物脱胶菌株筛选及其应用效果初探[J]. 南方农业学报,2011,42(08):944-947.

[5] Mei Y Z,Chen Y R,Zhai R Y,et al. Cloning,purification and biochemical properties of a thermostable pectinase from Bacillus halodurans M29[J]. Journal of Molecular Catalysis B:Enzymatic,2013,33:77-81.

[6] Soriano M,Diaz P,Pasto F. Pectate lyase C from Bacillus subtilis:A novel endo-cleaving enzyme with activity on highly methylated pectin[J]. Microbiology,2006,152(3):617-625.

[7] 朱珣之,李强,华楠,等. 磁性氧化石墨烯固定化果胶酶[J]. 化工进展,2014,33(12):3324-3328.

[8] 张健红,李寅,刘和,等. 一株碱性果胶酶高产细菌的分离、系统发育分析和产酶条件的初步优化[J]. 应用与环境生物学报, 2005(3):354-358.

[9] Kashyap D R,Vohra P K,Chopra S,et al. Applications of pectinases in the commercial sector:A review[J]. Bioresource Technology,2001,77(3):215-227.

[10] Gummadi S N,Kumar D S. Microbial pectic transeliminases[J]. Biotechnology Letters,2005,27(7):451-458.

[11] 刘龙,汪志浩,张东旭,等. 碱性果胶酶的生物制造及其在纺织工业清洁生产中的应用研究进展[J]. 生物工程学报,2009,25(12):1819-1828.

[12] 黄小龙,孙焕良,谢达平,等. 亚麻微生物脱胶菌种的筛选与鉴定[J]. 生物学杂志,2004,21(1):20-22.

[13] 熊元林. 微生物学实验[M]. 武汉:华中师范大学出版社,2008:130-131.

[14] 王俊刚,张树珍,杨本鹏,等. 3,5-二硝基水杨酸(DNS)法测定甘蔗茎节总糖和还原糖含量[J]. 甘蔗糖业,2008(5):45-49.

[15] 林静,谭晓明,王臻,等. 亚麻纤维脱胶菌的筛选及脱胶效果研究[J]. 生物技术通报,2011(5):132-136.

[16] Miller G L. Use of dinitrosalicylic acid reagent for determination of reducing sugar[J]. Analytical Chemistry,1959,31(3):426-428.

[17] 翟秋梅,薛卫巍,薛永常,等. 枯草芽孢杆菌FM208849产果胶酶发酵条件的优化[J]. 大连工业大学学报,2010,29(2):93-97.

[18] Payasi A,Sanwal R,Sanwal G G. Microbial pectate lyases:Characterization and enzymological properties[J]. World Journal of Microbiology and Biotechnology,2009,25:1-14.

[19] 陈丽娜,应向贤,薛群,等. 细菌碱性果胶酶的酶学特性及其应用研究[J]. 中国生物工程杂志,2010,30(4):125-130.

[20] Basu S,Roy A,Ghosh A,et al. Arg235 is an essential catalytic residue of Bacillus pumilus DKS1 pectate lyase to degum ramie fibre[J]. Biodegradation,2011,22(1):153-161.

香蕉假茎中产碱性果胶酶细菌的筛选发酵优化与酶学性质

Isolation,fermentation optimization and enzymatic properties of a pectinase producing strain from banana pesudostem

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