双水相萃取藻蓝蛋白的机理

doi:10.16085/j.issn.1000-6613.2015.09.032

化工进展 ›› 2015, Vol. 34 ›› Issue (09): 3406-3414.DOI: 10.16085/j.issn.1000-6613.2015.09.032

双水相萃取藻蓝蛋白的机理

邵琳琳¹, 张烜¹, 宋佳玉¹, 崔正刚¹, 王峰^1,2

1. 江南大学化学与材料工程学院, 江苏无锡 214122;
2. 江南大学食品胶体与生物技术教育部重点实验室, 江苏无锡 214122

收稿日期:2015-01-14 修回日期:2015-03-12 出版日期:2015-09-05 发布日期:2015-09-05
通讯作者: 王峰,副教授,主要从事生物化工研究。E-mail:fwang@jiangnan.edu.cn
作者简介:邵琳琳(1990-),女,硕士研究生。E-mail:shaolinlin0310@163.com
基金资助:
中央大学基础研究基金项目(JUSRP211A09)。

Mechanism of the aqueous two-phase extraction of phycocyanin

SHAO Linlin¹, ZHANG Xuan¹, SONG Jiayu¹, CUI Zhenggang¹, WANG Feng^1,2

1. School of Chemistry and Material Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China;
2. The Key Laboratory of Food Colloids and Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China

Received:2015-01-14 Revised:2015-03-12 Online:2015-09-05 Published:2015-09-05

摘要/Abstract

摘要： 采用荧光光谱的方法对双水相萃取藻蓝蛋白(PC)的机理进行了研究。不同分子量的聚乙二醇(PEG)对PC均有荧光猝灭作用。PEG与PC相互作用的热力学研究结果表明,PEG1000与PC的结合常数大于PEG2000和PEG4000,两者是熵驱动下的疏水作用力结合。不同的无机盐对PEG1000与PC结合的影响不同,其中Na₂SO₄使PEG1000-PC体系熵增效果最显著。同步荧光光谱的研究表明,PEG1000使PC色氨酸残基微环境的疏水性增强;Na₂SO₄通过促进PEG1000与PC的结合而使色氨酸残基微环境的疏水性进一步增强。单因素扫描实验的结果表明,PEG1000/Na₂SO₄体系双水相萃取纯化藻蓝蛋白(PC)的能力最强,符合PEG1000与PC分子相互作用的热力学规律。

关键词: 双水相萃取, 藻蓝蛋白, 热力学, 纯化

Abstract: The mechanism for aqueous two-phase extraction of phycocyanin (PC) was studied using florescence spectrum. The fluorescence of PC can be quenched by the addition of PEG despite the variation in molecular weights of PEGs. The thermodynamic investigation found that the binding constant between PEG1000 and PC was higher than that between PEG2000 and PEG4000. The binding between PEG and PC was built through hydrophobic interaction driven by entropy. Furthermore,compared to other inorganic salts tested,the addition of Na₂SO₄ showed the most positive impact on entropy increase,promoting the combinative interaction between PEG and PC. Moreover,the investigations on synchronous fluorescence spectroscopy found that the hydrophobicity of micro-environment around tryptophan residues was enhanced by the presence of PEG1000,and the hydrophobicity of micro-environment was increased further by the addition of Na₂SO₄. The single-factor experiments demonstrated that PEG1000/Na₂SO₄ is the optimum aqueous two-phase extraction system in thermodynamics and practice for obtaining phycocyanin with the highest purity.

Key words: aqueous two-phase extraction, phycocyanin, thermodynamics, purification

中图分类号:

TQ02
Q67

邵琳琳, 张烜, 宋佳玉, 崔正刚, 王峰. 双水相萃取藻蓝蛋白的机理[J]. 化工进展, 2015, 34(09): 3406-3414.

SHAO Linlin, ZHANG Xuan, SONG Jiayu, CUI Zhenggang, WANG Feng. Mechanism of the aqueous two-phase extraction of phycocyanin[J]. Chemical Industry and Engineering Progree, 2015, 34(09): 3406-3414.

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双水相萃取藻蓝蛋白的机理

Mechanism of the aqueous two-phase extraction of phycocyanin

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