不同溶剂萃取小球藻油脂结构及组分差异

doi:10.16085/j.issn.1000-6613.2015.06.021

化工进展 ›› 2015, Vol. 34 ›› Issue (06): 1631-1635,1651.DOI: 10.16085/j.issn.1000-6613.2015.06.021

不同溶剂萃取小球藻油脂结构及组分差异

戴欣¹, 杨雪薇¹, 崔岩¹, 王波², 周连宁², 成家杨¹

1 北京大学深圳研究生院, 环境与能源学院, 深圳藻类新能源技术开发和应用工程实验室, 广东深圳 518055;
2 深圳市深港产学研环保工程技术股份有限公司, 广东深圳 518000

收稿日期:2014-11-02 修回日期:2014-11-24 出版日期:2015-06-05 发布日期:2015-06-05
通讯作者: 成家杨,博士,教授,北京大学环境与能源学院院长,美国北卡罗莱那州立大学终身教授。E-mail:chengjy@pkusz.edu.cn。
作者简介:戴欣(1988—),男,硕士研究生。E-mail:buhuifei@pku.edu.cn。
基金资助:
国家海洋公益性行业科研专项(201305022)、中国博士后科学基金(2012M520106)、中国博士后科学基金第六批特别资助项目(2013T60025)及深圳市战略新兴产业发展专项(JCYJ20130329175919527, CXZZ20120618111150009)项目。

Research on the lipid structure and compound differences of the extraction of Chlorella with different solvents

DAI Xin¹, YANG Xuewei¹, CUI Yan¹, WANG Bo², ZHOU Lianning², CHENG Jiayang¹

1 Shenzen Engineering Laboratory for Algal Biofuel Technology Development and Application, School of Environment and Energy, Peking University-Shenzhen Graduate School, Shenzhen 518055, Guangdong, China;
2 IER Environmental Protection Engineering Technique Co., Ltd., Shenzhen 518000, Guangdong, China

Received:2014-11-02 Revised:2014-11-24 Online:2015-06-05 Published:2015-06-05

摘要/Abstract

摘要： 随着化石能源枯竭, 微藻生物柴油日益成为研究热点, 但对藻油的高效、高选择性萃取仍存在技术瓶颈。该文选择不同沸点、极性和油水分配系数的有机溶剂萃取微藻藻油, 并通过FTIR及GC-MS对微藻生物质结构及藻油组分差异进行明晰。结果表明:乙腈和正庚烷对藻油的提取率最高, 分别为22.10%和18.71%, 且与温度正相关, 但是溶剂的极性和油水分离系数对其萃取率无显著影响, 主要取决于所萃取产物的组分及结构;对微藻生物质结构及藻油组分研究表明不同溶剂对烃类、醇类、酯类等不同物质的萃取有较强的选择性;二氯甲烷和丙酮对烃类的萃取率高, 为72.74%和69.50%, 正庚烷对酯类的萃取率较高, 为80.46%, 乙腈对胺类的萃取率较高, 为58.52%, 乙酸乙酯对醇类的萃取率较高, 为24.65%。

关键词: 微藻, 生物柴油, 溶剂萃取

Abstract: With the depleting supplies of fossil fuels, microalgae have attracted an increasing attention as a feedstock for biodiesel in recent years. However, a bottleneck issue with algal biodiesel manufacturing is the lack of efficient and highly selective extraction methods. According to literature, six organic solvents with different boiling points, polarity and oil-water partition coefficients were applied for lipid extraction from microalgae. The biomass structure and extractive compound were analyzed by FT-IR and GC-MS. The results showed that acetonitrile and n-heptane had the highest extraction yield with the value of 22.10% and 18.71%, respectively. The extraction yield was positively correlated to temperature. And the composition and structure of extraction products were the main factors that may influence extraction yield, while polarity and oil-water partition coefficient of the solvent did not affect extraction yield directly. The analysis of microalgae biomass structure and extraction product composition indicated that different solvents presented highly selectivity for the extraction of different compounds, like hydrocarbon, alcohol, and ester. Dichloromethane and acetone had the highest extraction yield on hydrocarbon, with up to 72.74% and 69.50%, n-heptane had the highest extraction yield on ester(80.46%), acetonitrile had the highest extraction yield on amine(58.52%), and ethyl acetate had the highest extraction on alcohol(24.65%).

Key words: microalgae, biodiesel, solvent extraction

中图分类号:

Q819

戴欣, 杨雪薇, 崔岩, 王波, 周连宁, 成家杨. 不同溶剂萃取小球藻油脂结构及组分差异[J]. 化工进展, 2015, 34(06): 1631-1635,1651.

DAI Xin, YANG Xuewei, CUI Yan, WANG Bo, ZHOU Lianning, CHENG Jiayang. Research on the lipid structure and compound differences of the extraction of Chlorella with different solvents[J]. Chemical Industry and Engineering Progree, 2015, 34(06): 1631-1635,1651.

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不同溶剂萃取小球藻油脂结构及组分差异

Research on the lipid structure and compound differences of the extraction of Chlorella with different solvents

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