Abstract: In order to determine the optimal preparation condition of chitosan composite sponge，the factors and their levels were established first by single factor experiment，and then response surface method was used on the basis of single factor experiment via design concept of the Box-Benhnken center combination experiment. The result shows that，the optimal preparation condition of chitosan composite sponge is that the stirring speed is 541 r/min，the mass percent of sodium hyaluronate is 14%，the ratio of chitosan and sodium alginate is 3.71∶1，the theoretical tensile strength is 0.1652 MPa，and the actual tensile strength is 0.1709 MPa. The preparation technology of chitosan composite sponge has been optimized by response surface method. The sponge material has a good performance on water absorption and water retention. The vesicular structure of the sponge is uniform and multi-level.

Key words: response surface analysis, chitosan composite sponge, preparation, optimization

摘要： 为了确定壳聚糖复合海绵的最佳制备工艺条件，通过单因素实验选取实验因素与水平，根据Box-Benhnken的中心组合实验设计原理，在单因素试验的基础上采用三因素三水平的响应面分析法，依据回归分析确定各工艺条件的影响因子，以复合海绵材料的拉伸强度为响应值作响应面和等值曲线图。壳聚糖复合海绵材料的最佳工艺条件为：电机搅拌速度为541 r/min，透明质酸钠质量分数为14%，壳聚糖与海藻酸钠的质量配比为3.71∶1，得到理论拉伸强度为0.1652 MPa，实际拉伸强度为0.1709 MPa。通过响应面分析法确定了壳聚糖复合海绵的最佳制备工艺，表明该海绵材料具有均匀的多孔与多层次的结构，吸水、保水性能均良好。

关键词: 响应面分析, 壳聚糖复合海绵, 制备, 优化